【摘要】：目的： 探討建立簡捷、經(jīng)濟實用、穩(wěn)定可靠的大鼠膽管良性狹窄動物模型的方法,利于對膽管良性狹窄的發(fā)生機制和有效治療方法的研究。 方法： 49只雌性SD (Sprague Dawley)大鼠體重220-260克,平均240±10 g,隨機將其分為實驗組21只、實驗對照組21只和空白對照組7只,所有實驗動物均采用3%的戊巴比妥注射液進行腹腔麻醉。實驗組21只動物進行腹腔麻醉后上腹劍突下正中切口開腹,顯露膽總管后在距離十二指腸上緣2cm處分離出膽總管長約1cm,其下面放置一厚約0.2cm的棉墊后滴注95%的酒精約0.7ml于膽管上持續(xù)2-3分鐘后關(guān)腹；實驗對照組以同樣的方法進行手術(shù),在膽管上滴注0.9%的生理鹽水后2-3分鐘關(guān)腹；空白對照組只是找出膽管后就關(guān)腹,不進行其他處理。術(shù)后觀察包括精神狀態(tài)、尿液顏色、進食情況;實驗?zāi)Ｐ徒M和實驗對照組術(shù)后7、14、21天共分三次每次分別取7只動物稱體重與及時抽血檢查肝功能(ALT、GGT、T-BIL、D-BIL),并切取部分肝臟和實驗處膽管行病理學(xué)檢查,21天組還進行膽道造影了解膽管梗阻情況。統(tǒng)計學(xué)采用SPSS軟件t檢驗及多元方差分析。 結(jié)果： 術(shù)后1hSD大鼠能翻身活動,3-4h后自主飲水,逐漸恢復(fù)飲食。①從術(shù)前到術(shù)后21天,實驗造模組檢測血清丙氨酸氨基轉(zhuǎn)移酶(ALT)、γ-谷氨酰轉(zhuǎn)移酶(γ-glutamyl transferase,GGT)、血清總膽紅素(T-BIL)、血清直接膽紅素(D-BIL)等指標(biāo)有明顯變化(P0.05)；而對照組和空白對照組無顯著變化。②各時間點處死大鼠切取肝臟及膽管行病理學(xué)檢查,實驗造模組隨著時間推移發(fā)現(xiàn)肝細胞有不同程度損傷,膽管組織部分纖維增生；而對照組和空白對照組術(shù)前與術(shù)后對比無明顯變化。③術(shù)后21天膽道造影發(fā)現(xiàn),實驗造模組膽管有狹窄表現(xiàn)；而對照組和空白對照組無膽管狹窄出現(xiàn)。通過上述比較,實驗組與對照組差異有統(tǒng)計學(xué)意義(P0.05),對照組與空白對照組無統(tǒng)計學(xué)意義(P0.05)。 結(jié)論： 通過肝外膽管外滴注95%的乙醇制造大鼠膽管良性狹窄模型是可行的,能夠為膽管良性狹窄的研究提供另一種動物模型。
[Abstract]:Objective: to establish a simple, economical and reliable animal model of benign stricture of bile duct in rats, and to study the mechanism and effective treatment of benign stricture of bile duct. Methods: 49 female SD (Sprague Dawley) rats weighing 220-260 g (mean 240 鹵10 g) were randomly divided into experimental group (n = 21), experimental control group (n = 21) and blank control group (n = 7). All experimental animals were anesthetized with 3% pentobarbital injection. After abdominal anaesthesia, 21 animals in the experimental group were treated with abdominal anesthesia. The length of the common bile duct was about 1 cm after exposure of the common bile duct, which was about 1 cm from the upper edge of the duodenum, and the length of the common bile duct was about 1 cm from the upper edge of the duodenum. After placing a cotton pad about 0.2cm thick, 95% alcohol was infused into the bile duct for 2-3 minutes and then closed the abdomen. The control group was operated in the same way, and the bile duct was dripped with 0.9% normal saline for 2-3 minutes after abdominal closure, while the blank control group only found the bile duct and closed the abdomen without any other treatment. Postoperative observations included mental state, urine color, and food intake. The experimental model group and the experimental control group were divided into three groups, 7 animals were weighed and the liver function (ALT,GGT,T-BIL,D-BIL) was examined in time, and some of the liver and the bile duct were taken out for pathological examination. Cholangiography was also performed in the 21-day group to find out the biliary obstruction. SPSS software t test and multivariate analysis of variance were used in statistics. Results: after operation, 1hSD rats were able to turn over and drink their own water 3-4 hours later. 1 from pre-operation to 21st day after operation, serum alanine aminotransferase (ALT), was detected in the experimental model group. 緯-glutamyl transferase (緯-glutamyl transferase,GGT), serum total bilirubin (T-BIL) and serum direct bilirubin (D-BIL) were significantly changed (P0.05). However, there were no significant changes in the control group and the blank control group. 2 the rats were killed at each time point to take the liver and bile duct for pathological examination. The experimental model group found that the liver cells were damaged in varying degrees and some of the bile duct fibers proliferated with the passage of time. The contrast between the control group and the blank control group showed no significant changes before and after operation. 3 21 days after operation, bile duct stenosis was found in the experimental model group, but no biliary stricture was found in the control group and the blank control group. Through the above comparison, the difference between the experimental group and the control group was statistically significant (P0.05), while the control group and the blank control group had no statistical significance (P0.05). Conclusion: it is feasible to establish a rat model of benign bile duct stenosis by extrahepatic infusion of 95% ethanol, which can provide another animal model for the study of benign bile duct stenosis.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R657.4;R-332

【參考文獻】

相關(guān)期刊論文 前10條

1 何中林;陳平;閆洪濤;還勇為;;膽汁刺激與良性膽管狹窄形成機制的試驗研究[J];重慶醫(yī)學(xué);2008年15期

2 唐仕芳;朱洪春;李華強;史源;趙錦寧;;轉(zhuǎn)化生長因子-β_1在新生大鼠高濃度氧致肺損傷中的作用研究[J];重慶醫(yī)學(xué);2009年04期

3 劉莉;孫彬;兌丹華;;平滑肌肌動蛋白在膽管機械性損傷與電灼傷中的表達[J];第三軍醫(yī)大學(xué)學(xué)報;2009年05期

4 徐軍,耿智敏,劉青光,向國安,劉效恭,韓慶,王康敏;膽管損傷愈合過程中顯微結(jié)構(gòu)和超微結(jié)構(gòu)的變化[J];第四軍醫(yī)大學(xué)學(xué)報;2002年16期

5 張守華;廖彩仙;張春興;鄧紅;朱航;雷蕾;藥晨;;小鼠梗阻性黃疸模型的建立及動態(tài)觀察[J];南方醫(yī)科大學(xué)學(xué)報;2008年09期

6 郭興軍;鄭啟昌;張磊;宋自芳;;記憶合金支架在肝門部膽管狹窄手術(shù)治療中的應(yīng)用[J];腹部外科;2006年05期

7 孫楊忠,耿小平;阻塞性黃疸大鼠膽總管直徑、壓力及血清膽紅素代謝變化的觀察[J];肝膽外科雜志;2003年02期

8 付慶江;屈順喜;吳志宇;梁家銘;劉希寧;劉四清;曹立瀛;;肝圓韌帶修復(fù)良性膽管狹窄14例報告[J];肝膽外科雜志;2009年03期

9 李定耀,李明主,李明;膽道內(nèi)支架治療肝門部膽管狹窄九例[J];肝膽胰外科雜志;2000年01期

10 王秋實;吳德全;孫凌宇;王琛;李國東;姚磊;;γ-干擾素對兔膽總管愈合過程中細胞增殖和轉(zhuǎn)化的影響[J];肝膽胰外科雜志;2006年02期

，

本文編號：2334309