【摘要】：目的:建立空腸彎曲菌(Campylobacter jejuni,Cj)感染致急性周圍神經(jīng)病巴馬香豬模型,初步探討以空腸彎曲菌為感染源引起繼發(fā)免疫相關(guān)疾病的發(fā)病模式,為研究急性運動感覺性軸索性神經(jīng)病(acute motor sensory axonal neuropathy, AMSAN)的發(fā)病機制、其病理生理及空腸彎曲菌的生長特性、感染特性奠定研究模型基礎(chǔ)。 方法:以吉蘭-巴雷綜合征(Guillain-Barre syndrome,GBS)患者分離培養(yǎng)空腸彎曲菌,經(jīng)中國疾病預(yù)防控制中心鑒定為空腸彎曲菌菌株,并經(jīng)美國疾病預(yù)防控制中心鑒定血清型為penner O:19型。用哥倫比亞血培養(yǎng)基于42℃,10%CO2、5%O2、85%N2培養(yǎng)空腸彎曲菌。純化菌種制備1×106菌懸液灌喂巴馬香豬,灌喂前禁食12小時、禁水4小時。分別于灌喂前后分離、鑒定胃腸道菌群。自灌喂當(dāng)日起每日觀察巴馬香豬的臨床癥狀:飲食、大便及肢體活動情況。對出現(xiàn)肢體活動障礙巴馬香豬自出現(xiàn)癥狀7天內(nèi)取材其脊神經(jīng)根、坐骨神經(jīng)及臂叢神經(jīng),進行餓酸染色、鍍銀染色、Luxol-fast-blue染色、免疫組織化學(xué)、透射電子顯微鏡進行神經(jīng)病理觀察。 結(jié)果:空腸彎曲菌自然感染巴馬香豬后,模型動物于1周內(nèi)出現(xiàn)腹脹、腹瀉,2周左右出現(xiàn)肢體活動障礙;神經(jīng)病理檢查可觀察到微管、微絲減少,軸索節(jié)段性萎縮,髓鞘示節(jié)段性回縮,未見脫髓鞘樣變性。神經(jīng)病理學(xué)初步診斷為急性運動、感覺軸索性神經(jīng)病。 結(jié)論:空腸彎曲菌感染巴馬香豬可致急性周圍神經(jīng)病,巴馬香豬可以作為AMSAN研究的模型動物。初步探討了神經(jīng)病理檢查方法。
[Abstract]:Objective: to establish a Bama pig model of acute peripheral neuropathy caused by campylobacter jejuni (Campylobacter jejuni,Cj) infection and to explore the pathogenesis of secondary immune-related diseases caused by Campylobacter jejuni as the source of infection. In order to study the pathogenesis of acute motor sensory axonal neuropathy (acute motor sensory axonal neuropathy, AMSAN), its pathophysiology, the growth characteristics of Campylobacter jejuni and the infection characteristics, the model was established. Methods: campylobacter jejuni was isolated from patients with Guillain-Barre syndrome (Guillain-Barre syndrome,GBS) and identified as Campylobacter jejuni by the China Center for Disease Control and Prevention. The serotype of penner O: 19 was identified by the Centers for Disease Control and Prevention. Campylobacter jejuni was cultured in Colombian blood at 42 鈩，

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