發(fā)布時間：2018-11-08 20:44

【摘要】：目的:本研究在中醫(yī)學(xué)辨證思維指導(dǎo)下,根據(jù)血瘀證理論探索和改良造模方法,采用多因素復(fù)合法建立血瘀型肝纖維化病證結(jié)合大鼠模型。方法:通過二甲基亞硝胺、小牛血清白蛋白和去甲腎上腺素注射液聯(lián)合注射,外加乙醇灌胃及高脂低蛋白飼料喂養(yǎng),多因素聯(lián)合構(gòu)建血瘀型肝纖維化大鼠模型。造模結(jié)束觀察大鼠的死亡率,評價中醫(yī)血瘀證證候,進行證候等級評分,觀察肝臟病理學(xué)大體形態(tài),檢測肝功能改變,采用免疫組化法觀察Ⅰ型和Ⅲ型膠原、α-平滑肌肌動蛋白(α-SMA)3種膠原蛋白的表達情況、放射酶免法血清學(xué)檢測大鼠肝纖維化4項——HA、P3NP、LN、CⅣ的含量。將以上結(jié)果與傳統(tǒng)四氯化碳(CCl4)單因素造模法進行比較。結(jié)果:血瘀證組:①大鼠的死亡率為20%;②大鼠出現(xiàn)瘀斑、舌紫暗、便溏等典型的中醫(yī)血瘀證證候,血瘀證證候等級評分高;③病理學(xué)大體形態(tài)觀察發(fā)現(xiàn)肝臟出現(xiàn)表面暗白、密布灰白結(jié)節(jié)、質(zhì)硬脆等纖維樣變化;④血清學(xué)肝功能檢測發(fā)現(xiàn)模型大鼠的ALT和AST,以及TBIL、DBIL及IBIL的含量均顯著增加;⑤肝臟Ⅰ型和Ⅲ型膠原、α-SMA 3種膠原蛋白的表達水平顯著增高;⑥大鼠的血清肝纖維化4項——HA、P3NP、LN、CⅣ的含量均顯著增加。與CCl4模型組相比,多因素復(fù)合法所致血瘀證模型組以上各項結(jié)果(除了死亡率及肝功能外)更為明顯,結(jié)果有統(tǒng)計學(xué)差異(P0.05)。結(jié)論:多因素復(fù)合造模方法能有效地改善CCl4造模法的高死亡率缺點;模型動物同時具備了疾病與證候雙重特征,既符合中醫(yī)血瘀證證候的基本特點,又與西醫(yī)肝纖維化病理特征相一致。
[Abstract]:Objective: under the guidance of dialectical thinking of traditional Chinese medicine (TCM), according to the theory of blood stasis syndrome and improving the method of making model, the model of liver fibrosis of blood stasis type combined with syndrome of liver fibrosis of blood stasis type was established by multifactor compound method. Methods: the rat model of hepatic fibrosis was established by combined injection of dimethylnitrosamine, bovine serum albumin and norepinephrine injection plus ethanol and high fat and low protein diet. At the end of the model, the mortality of rats was observed, the syndromes of blood stasis syndrome of traditional Chinese medicine were evaluated, the grading of syndromes, the gross morphology of liver pathology and the changes of liver function were observed, and the type 鈪，

本文編號：2319633