【摘要】：目的探討貴州省3個(gè)少數(shù)民族線粒體DNA(mtDNA)9bp序列缺失頻率情況。方法隨機(jī)選取貴州省雷山縣苗族(69例)、荔波縣布依族(70例)、荔波縣水族(44例)共183例男性血液標(biāo)本,應(yīng)用PCR及直接測(cè)序檢測(cè)線粒體DNA 9bp序列缺失情況。結(jié)果在貴州省3個(gè)少數(shù)民族樣本中發(fā)現(xiàn)標(biāo)準(zhǔn)型、缺失型、3型,缺失頻率最高為荔波縣水族(40.91%),雷山縣苗族中檢出3型1例,3個(gè)少數(shù)民族群體間mtDNA 9bp缺失頻率比較差異無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論 3個(gè)世居少數(shù)民族mtDNA 9bp缺失頻率不同,水族的遺傳變異較大,水族與苗族的親緣關(guān)系相比水族與布依族較近。
[Abstract]:Objective to investigate the frequency of mitochondrial DNA (mtDNA) 9bp deletion in three minority nationalities in Guizhou province. Methods A total of 183 male blood samples were randomly selected from Miao nationality (69 cases), Buyi nationality (70 cases) and aquatic nationality (44 cases) in Leishan County, Guizhou Province. Mitochondrial DNA 9bp deletion was detected by PCR and direct sequencing. Results Standard type, deletion type and 3 type were found in 3 minority nationality samples in Guizhou province. The highest frequency of deletion was found in Shui nationality of Libo County (40.91%), and in one case of Miao nationality in Lishan County. There was no significant difference in the frequency of mtDNA 9bp deletion among the three ethnic groups (P0.05). Conclusion the frequency of mtDNA 9bp deletion in the three ethnic minorities is different, the genetic variation of the aquarium is greater, and the genetic relationship between the Shui and Miao is closer than that between the Shui and Buyi.
【作者單位】： 地方病與少數(shù)民族性疾病教育部重點(diǎn)實(shí)驗(yàn)室/貴州醫(yī)科大學(xué)分子生物學(xué)重點(diǎn)實(shí)驗(yàn)室;
【基金】：貴州省“2011協(xié)同創(chuàng)新中心”項(xiàng)目(黔教合協(xié)同創(chuàng)新中心[2014]06號(hào)) 貴州省科技廳基金資助項(xiàng)目(黔科合J字[2011]2119號(hào))
【分類號(hào)】：R394

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