人外周血單核細(xì)胞遷移分化為角膜上皮樣細(xì)胞初步研究
發(fā)布時間：2018-10-29 09:52
【摘要】：目的：通過對單核遷移細(xì)胞行體外遷移,探索外周血單核細(xì)胞在新鮮角膜緣干細(xì)胞和角膜上皮細(xì)胞微環(huán)境中誘導(dǎo)下,向角膜上皮細(xì)胞跨胚層分化的可能性。 方法：1、體外模擬白細(xì)胞遷移過程的模型：細(xì)胞培養(yǎng)插入皿上室(8μm孔徑)鋪上Ⅳ型膠原蛋白后插入六空板內(nèi),種上血管內(nèi)皮細(xì)胞。加上單核細(xì)胞懸液于上室,此時下室加入含650ng/ml MCP-1的培養(yǎng)基。48小時后,移去細(xì)胞培養(yǎng)插入皿,收集下室細(xì)胞。為了觀察對此模型的影響因素,將實驗分為二組：第一組：上室加入含體積分?jǐn)?shù)10%FBS的基礎(chǔ)培養(yǎng)基：第二組：上室不加入含F(xiàn)BS的基礎(chǔ)培養(yǎng)基。向兩組下室分別添加濃度為650ng/mlMCP-1和Ong/ml MCP-1,48小時后,移去插入皿,收集下室細(xì)胞。倒置相差顯微鏡觀察遷移后細(xì)胞形態(tài),流式細(xì)胞術(shù)分別檢測遷移前后單核細(xì)胞表面相對特異性標(biāo)志：CD14。 2、將收集的遷移細(xì)胞置于新鮮角膜緣干細(xì)胞和角膜上皮細(xì)胞微環(huán)境中共培養(yǎng)7d,收集細(xì)胞,流式細(xì)胞術(shù)檢測誘導(dǎo)前后細(xì)胞表面標(biāo)志物細(xì)胞角蛋白(cytokeratin)、波形蛋白(vimentin)的陽性率。 結(jié)果：1、流式細(xì)胞儀檢測,遷移模型模擬前測得上室CD14單核細(xì)胞陽性率為19.40%,遷移模型模擬后測得上室CD14單核細(xì)胞陽性率為1.44%。2、流式細(xì)胞術(shù)檢測,微環(huán)境誘導(dǎo)前遷移細(xì)胞Cytokeratin陽性率為1.64%、Vimentin陽性率為96.29%,微環(huán)境誘導(dǎo)后遷移細(xì)胞Cytokeratin陽性率為11.30%, Vimentin陽性率為31.55%。 結(jié)論：1、體外模擬白細(xì)胞遷移過程后,觀察到人外周血單核細(xì)胞在不加入FBS、Ⅳcollagen濃度為lug、MCP-1濃度為650ng/ml實驗條件時貼壁細(xì)胞較多。2、通過微環(huán)境誘導(dǎo)前后遷移單核細(xì)胞CK、Vimentin陽性率的改變初步認(rèn)為人外周血單核細(xì)胞可能具備跨胚層分化的潛能。
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【學(xué)位授予單位】：暨南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R329
本文編號：2297360
[Abstract]:% E7%9B%AE%E7%9A%84%EF%BC%9A%E9%80%9A%E8%BF%87%E5%AF%B9%E5%8D%95%E6%A0%B8%E8%BF%81%E7%A7%BB%E7%BB%86%E8%83 % 9E%E8%A1%8C%E4%BD%93%E5%A4%96%E8%BF%81%E7%A7%BB%2C%E6%8E%A2%E7%B4%A2%E5%A4%96%E5%91%A8%E8%A1%80%E5%8D%95 % E6%A0%B8%E7%BB%86%E8%83%9E%E5%9C%A8%E6%96%B0%E9%B2%9C%E8%A7%92%E8%86%9C%E7%BC%98%E5%B9%B2%E7%BB%86%E8%83 % 9E%E5%92%8C%E8%A7%92%E8%86%9C%E4%B8%8A%E7%9A%AE%E7%BB%86%E8%83%9E%E5%BE%AE%E7%8E%AF%E5%A2%83%E4%B8%AD%E8 % AF%B1%E5%AF%BC%E4%B8%8B%2C%E5%90%91%E8%A7%92%E8%86%9C%E4%B8%8A%E7%9A%AE%E7%BB%86%E8%83%9E%E8%B7%A8%E8%83 % 9A8A82E52E552, E582, E696B9, E6B3%, you have the right side, including the following: 3%. 95EFBCCC2C33E5, including E8BF789, E83B1, and E8BF799, E83B9, F8E799, 839E9, the right side of the side, including E8BF8BF88BF87% and E8BF7BF88BF87%, including E88BF88BF88BF87%, including E8BF7BF88BF87% and E8BF87%, including E8BF7BF88BF87%. E7A8B, E7A88B, E7A88888A888B, E7A888B88A8888E8839E8888A8888E8888A888A888E88B8A88A88A88A88A88B8A88B8A88A88A88A8A88A88A88A88A88E88E88A88A88A88E88E88E88E88E88E88A8 BF%E4%B8%8A%E5%AE%A4%288%CE%BCm%E5%AD%94%E5%BE%84%29%E9%93%BA%E4%B8%8A%E2%85%A3%E5%9E%8B%E8%83%B6%E5%8E%9F% E89B, E8B5908, including E8B88A8A8A8E8%, including E88B88A8A8A8A8A8E8%, including E88B88A8A8A8A8A88A88A88A8A88A88A88A88A88A88A88A888A888A888A8888A8888A8888A8A8A88A8% of the head, including 8% of them, including 8%. A1%80%E7%AE%A1%E5%86%85%E7%9A%AE%E7%BB%86%E8%83%9E.%E5%8A%A0%E4%B8%8A%E5%8D%95%E6%A0%B8%E7%BB%86%E8%83%9E % E6%82%AC%E6%B6%B2%E4%BA%8E%E4%B8%8A%E5%AE%A4%2C%E6%AD%A4%E6%97%B6%E4%B8%8B%E5%AE%A4%E5%8A%A0%E5%85%A5%E5 % 90%AB650ng%2Fml%20MCP-1%E7%9A%84%E5%9F%B9%E5%85%BB%E5%9F%BA.48%E5%B0%8F%E6%97%B6%E5%90%8E%2C%E7%A7%BB%E5%8E%BB%E7 % BB%86%E8%83%9E%E5%9F%B9%E5%85%BB%E6%8F%92%E5%85%A5%E7%9A%BF%2C%E6%94%B6%E9%9B%86%E4%B8%8B%E5%AE%A4%E7%BB % 86E839E.839E.8E.839E.8E.839E.9E.4E.8E89E.89E.88E4E 839E.8E4E 839E.839E.8E.89E.839E.4E839E.839E.4E839E.4E839E.4E839E.839E.4E839E.839E.8E.89E.83E.4E.8E89E.89E.4E839E.8E.89E.839E.4E.839E.839E.8 E538D8DB4, E538D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8 E538D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8 AC%E4%B8%80%E7%BB%84%EF%BC%9A%E4%B8%8A%E5%AE%A4%E5%8A%A0%E5%85%A5%E5%90%AB%E4%BD%93%E7%A7%AF%E5%88%86% E695B010A9FBS9A5, including E7BB84EF8EF%, E795B010E9F9F9FBAEFB9F580E580FB9FB9F580E580FB5B9F5E580E580E7B5B9F7E7E7E7B7A5E7E7E7E7E7E7B7A5A9A9A9A9A9A9FB7E7B8E7E7B84EF5 and E7BB84EF8EF8%. BC%9A%E4%B8%8A%E5%AE%A4%E4%B8%8D%E5%8A%A0%E5%85%A5%E5%90%ABFBS%E7%9A%84%E5%9F%BA%E7%A1%80%E5%9F%B9%E5%85%BB% E5%9F%BA.%E5%90%91%E4%B8%A4%E7%BB%84%E4%B8%8B%E5%AE%A4%E5%88%86%E5%88%AB%E6%B7%BB%E5%8A%A0%E6%B5%93%E5%BA % A6%E4%B8%BA650ng%2FmlMCP-1%E5%92%8COng%2Fml%20MCP-1%2C48%E5%B0%8F%E6%97%B6%E5%90%8E%2C%E7%A7%BB%E5%8E%BB%E6%8F%92%E5%85%A5 % E79A BF2C2C79A BF2B8E79BF2B888B88E88E88092E88BE4E88BE4E88BE4B6B6B9B9B B8E7A9A9B8E7A7A7A5AE7A5AE7A7A5AE7A5AE7A5AE7A7AE7A% should be the right side of the side of B8B7E7A7A%. E6988BEE9C9C, including E88E7A7F7F5908E88E7F7F5908, including E88E7F7F7F790, including A782, E992, E989, and E5, and BDA2E680%, including E8, E7, F7, F5, E5, F5, and E5, the right, and right, right. 81C2CE6C8F, including 81C2C8F, including E7A7A7BB% and E7A7BB%, including the side of E7A7A7BB%, including A71E7A7BB%, A7A7BB%, and E7A7BB%, including A7A7BB%, including the following aspects: 81C2C2C2C8C8F2, ABE6A6A380E588E588E588E588E588E588E580E580E5338B, including the right side, right, right. E5898D8D8D9D9D8D9D8D8D8D8D8D8D8D8D8D8D8D8D88D88D88D88D88D8D88D88D88D8D88D88D88D8D8D88D88D8D8D8D8D8D8D8D8D8D8D8D8D8D9D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8D8 82%E6%80%A7%E6%A0%87%E5%BF%97%EF%BC%9ACD14.%0D%0A%0D%0A%0D%0A%092%2C%E5%B0%86%E6%94%B6%E9%9B%86%E7%9A%84%E8%BF % 81%E7%A7%BB%E7%BB%86%E8%83%9E%E7%BD%AE%E4%BA%8E%E6%96%B0%E9%B2%9C%E8%A7%92%E8%86%9C%E7%BC%98%E5%B9%B2%E7 % BB%86%E8%83%9E%E5%92%8C%E8%A7%92%E8%86%9C%E4%B8%8A%E7%9A%AE%E7%BB%86%E8%83%9E%E5%BE%AE%E7%8E%AF%E5%A2%83 % E4%B8%AD%E5%85%B1%E5%9F%B9%E5%85%BB7d%2C%E6%94%B6%E9%9B%86%E7%BB%86%E8%83%9E%2C%E6%B5%81%E5%BC%8F%E7%BB%86 % E8%83%9E%E6%9C%AF%E6%A3%80%E6%B5%8B%E8%AF%B1%E5%AF%BC%E5%89%8D%E5%90%8E%E7%BB%86%E8%83%9E%E8%A1%A8%E9%9D % A2%E6%A0%87%E5%BF%97%E7%89%A9%E7%BB%86%E8%83%9E%E8%A7%92%E8%9B%8B%E7%99%BD%28cytokeratin%29%2C%E6%B3%A2%E5%BD%A2%E8 % 9B8B8B8Bmentin29E729, including the side of 9B8B87.0D0A7D0A0A0D0A0D0A0D0A70D08E680A0D0D0D08E790D0D0D08E790D0D08E790D0D0D08E790D0D08E790D0D0D08E790D0D08E790D0D088E790D0D0D0D088E7909, including the following aspects: 9A12C2C2E6E6B8F, including E7C12C2C8C8F, including E83E380E88E88E83E88E88B5B5B, including E83E8E88E59E, including the side of the side, including the head of the bank, the right side, and the right side, right now, right, right. 8BE6A8A8A1and 8BE6A8A8A1, including 8D8E6A8A8A88B8B5B5E5E5E8E5E5A8E5E5A8E5E5A8E5E5A88B5B8B88B88A5A88A88A5A8A88A8A88A88A5A88A8A88A88A5A88A5A8A88A8A88A8A88A5A8A8A8A8A8A8A8A8A8A8 98B3E680A7E680A7E7E680E4E4B, and E6908E6908E6908E8E51A1E7A7A7B88B7A7A72E88A8E68E68E8E68E88E68E68E68E4and E6908E5908E5908E5908E5908E5908E5908E5908E8E5908E% E6B57B8B8A, including E6B97B88A, E7E78E7E788A7E78E88B8, including E5A7E78E88B8, including E5A8E78E78B8, including E5A8B7E88B8, E6B58B58B88B8, E6B97B58B8B8, E6B97B88B88B8, E6B97B7E88A8E78E7E7E8B8 and E88B8%. BA1.44%25.2%2C%E6%B5%81%E5%BC%8F%E7%BB%86%E8%83%9E%E6%9C%AF%E6%A3%80%E6%B5%8B%2C%E5%BE%AE%E7%8E%AF%E5%A2% 83E8E8A8A589 and E78E889, including E78E78E782, E8339ECy880E880B1, and 8DE8E88E589, including E78E889, E787 E887, E784%, B8BA1.64%, BBA1.64%, including BBA1.64%, B8BA1.64%, including BBA1.64%, including BBA1.64%. 252CVimentin E987 E998 E88BEA88E998 E78B680A7 E5908E5908E5908E5908E5908E590%, including E8E5BEA8E828E882, E78BEA8E828E889, including E78E87B1E680A7, including E5908E5908E5908E5908E590%. E8BF1, E8BF7, E7BF7, E7ECytokeratin, E98BF7, E787, E880B7, E7, B7, E7, and E7, 8E%87%E4%B8%BA31.55%25.%0D%0A%0D%0A%0D%0A%09%E7%BB%93%E8%AE%BA%EF%BC%9A1%2C%E4%BD%93%E5%A4%96%E6%A8%A1%E6%8B % 9F%E7%99%BD%E7%BB%86%E8%83%9E%E8%BF%81%E7%A7%BB%E8%BF%87%E7%A8%8B%E5%90%8E%2C%E8%A7%82%E5%AF%9F%E5%88%B0 % E4%BA%E5%A4%96%E5%91%A8%E8%A1%80%E5%8D%95%E6%A0%B8%E7%BB%86%E8%83%9E%E5%9C%A8%E4%B8%8D%E5%8A%A0%E5%85 % A5FBS%2C%E2%85%A3collagen%E6%B5%93%E5%BA%A6%E4%B8%BAlug%2CMCP-1%E6%B5%93%E5%BA%A6%E4%B8%BA650ng%2Fml%E5%AE%9E%E9%AA%8C%E6%9D%A1 % E4%BB%B6%E6%97%B6%E8%B4%E5%A3%81%E7%BB%86%E8%83%9E%E8%BE%83%E5%A4%9A.2%2C%E9%80%9A%E8%BF%87%E5%BE%AE % E7%8E%AF%E5%A2%83%E8%AF%B1%E5%AF%BC%E5%89%8D%E5%90%8E%E8%BF%81%E7%A7%BB%E5%8D%95%E6%A0%B8%E7%BB%86%E8%83 % 9ECK%2CVimentin%E9%98%B3%E6%80%A7%E7%8E%87%E7%9A%84%E6%94%B9%E5%8F%98%E5%88%9D%E6%AD%A5%E8%AE%A4%E4%B8%BA%E4%BA%BA % E5%A4%96%E5%91%A8%E8%A1%80%E5%8D%95%E6%A0%B8%E7%BB%86%E8%83%9E%E5%8F%AF%E8%83%BD%E5%85%B7%E5%A4%87%E8%B7 % A8, including the depth of 833, and the depth of E5B1822E5, and the rest of your arms, you put them in charge and put them around you and put them in the right place, and put them in the right place: E6BD9CE883 BD.
【學(xué)位授予單位】：暨南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R329
【參考文獻】
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