【摘要】：目的:探索寒性藥物復(fù)制大鼠虛寒證模型的造模及模型評價(jià)方法。通過觀察熱性中藥附子對虛寒證模型大鼠能量代謝及相關(guān)指標(biāo)的影響,分析附子作用趨勢、特點(diǎn)及規(guī)律,探尋反映實(shí)驗(yàn)動(dòng)物寒熱屬性的客觀指標(biāo),揭示其與寒熱藥物屬性的內(nèi)在聯(lián)系,為更好的指導(dǎo)臨床用藥奠定基礎(chǔ)。 方法:用生石膏、龍膽草、黃柏和知母水煎液灌胃14天復(fù)制虛寒證大鼠模型,給予附子水煎液灌胃治療7天,觀察虛寒模型大鼠的一般狀況、體溫、寒熱趨向等的變化;應(yīng)用代謝籠收集大鼠24小時(shí)食量、飲水量、尿量和糞量;應(yīng)用氧彈熱量計(jì)檢測大鼠攝入能、消化能、糞便能、可代謝能;采用酶聯(lián)免疫吸附法檢測大鼠血漿T3、T4、TSH、TRH含量;檢測空腹血糖含量;檢測肝組織糖原、乳酸含量、ATP酶活力、乳酸脫氫酶活力(LDH)、琥珀酸脫氫酶(SDH)活力;采用高效液相色譜法檢測肝組織ATP含量及肝細(xì)胞能荷。 結(jié)果:造模結(jié)束時(shí)模型組動(dòng)物毛色污濁,腹瀉明顯,體重、自主活動(dòng)及趾溫明顯降低,血漿丙酮酸、T3含量呈下降趨勢。附子可以顯著升高虛寒模型大鼠血漿T4含量及肝乳酸含量,明顯提升肝乳酸脫氫酶和肝Na~+-K~+-ATP酶活力,顯著升高肝細(xì)胞能荷;并且空腹血糖、體重、肛溫、飲水量、尿量、在熱箱的次數(shù)呈現(xiàn)一定的降低趨勢;糞重、在寒箱的次數(shù)、單位體重?cái)z入能、糞便能、單位體重糞便能,肝琥珀酸脫氫酶活力,ATP含量等指標(biāo)呈現(xiàn)一定的升高趨勢。 結(jié)論:寒性藥物生石膏、龍膽草、黃柏和知母配伍可成功復(fù)制大鼠虛寒證模型。熱性中藥附子能使虛寒模型大鼠能量代謝增強(qiáng),改善大鼠虛寒狀態(tài),其藥理作用的發(fā)揮同提高肝臟能量代謝和促進(jìn)甲狀腺激素的分泌關(guān)系密切。體重、體溫、血漿甲狀腺激素的含量、肝乳酸含量、ATP酶的活力、肝細(xì)胞能荷及空腹血糖含量等指標(biāo)可以作為寒熱證模型以及寒熱性中藥研究的重要評價(jià)指標(biāo)。
[Abstract]:Objective: to explore the model and evaluation method of the model of asthenia cold syndrome in rats induced by cold drugs. By observing the effect of aconite on energy metabolism and related indexes in rats with deficiency and cold syndrome, the effect trend, characteristics and rules of aconite were analyzed, and the objective indexes reflecting the cold and heat properties of experimental animals were explored. To reveal the internal relationship between the drug properties of cold and heat, and to lay a foundation for better guidance of clinical drug use. Methods: the rat model of deficiency cold syndrome was established by oral administration of raw gypsum, gentian grass, Phellodendron chinensis and Anemarrhena chinensis for 14 days. The rats were treated with water decoction of aconite for 7 days. The changes of general condition, body temperature, cold and heat tendency of rats with deficiency cold were observed. The intake energy, digestibility energy, fecal energy and metabolizable energy of rats were measured by oxygen bomb calorimeter, and the contents of T3T4 TSHTSHTRH in plasma were detected by enzyme-linked immunosorbent assay (Elisa). Fasting blood glucose content, liver glycogen, lactic acid content, ATP enzyme activity, lactate dehydrogenase activity, (LDH), succinate dehydrogenase (SDH) activity, liver tissue ATP content and hepatocyte energy charge were detected by HPLC. Results: at the end of the model making, the model group showed a marked decrease in hair color, diarrhea, body weight, spontaneous activity and toe temperature, and the contents of plasma pyruvate and T3 decreased. Aconite could significantly increase plasma T4 content and liver lactate content, increase the activity of liver lactate dehydrogenase and liver Na~ K- ATP enzyme, and increase hepatic energy charge, fasting blood glucose, body weight, anus temperature, drinking water and urine volume. In the hot box, the number of fecal weight, the number of intake energy per unit body weight, fecal energy, succinate dehydrogenase activity and ATP content showed a certain increasing trend. Conclusion: the cold drug gypsum, gentian grass, Phellodendron chinensis and Anemarrhena can be successfully used to replicate the model of deficiency cold syndrome in rats. Radix aconite can enhance the energy metabolism and improve the cold state in rats with deficiency cold. Its pharmacological effect is closely related to the enhancement of liver energy metabolism and the promotion of thyroid hormone secretion. Body weight, body temperature, plasma thyroid hormone content, liver lactic acid content, ATP enzyme activity, liver cell energy charge and fasting blood glucose content can be used as important evaluation indexes of cold and heat syndrome model and research of cold and heat Chinese medicine.
【學(xué)位授予單位】：山東中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R-332

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