發(fā)布時(shí)間：2018-10-12 08:11

【摘要】：目的觀察昆明種小鼠腹腔注射苯肼后外周血成熟紅細(xì)胞和網(wǎng)織紅細(xì)胞的變化規(guī)律,建立昆明種小鼠溶血性貧血模型。方法健康雄性昆明種小鼠18只,隨機(jī)分為溶劑對(duì)照組、低劑量苯肼組和高劑量苯肼組,每組6只。對(duì)照組于第0天經(jīng)腹腔注射生理鹽水,低劑量苯肼組和高劑量苯肼組動(dòng)物分別經(jīng)腹腔一次注射30和60 mg/kg·bw的苯肼,之后每天鼠尾采血進(jìn)行紅細(xì)胞計(jì)數(shù)和網(wǎng)織紅細(xì)胞計(jì)數(shù)。結(jié)果苯肼注射后,低劑量苯肼組和高劑量苯肼組小鼠外周血紅細(xì)胞總數(shù)與成熟紅細(xì)胞總數(shù)均逐漸下降,分別在第5天和第4天達(dá)到最低,且高劑量苯肼組下降的程度顯著高于低劑量苯肼組;此后,紅細(xì)胞總數(shù)與成熟紅細(xì)胞總數(shù)逐漸開始回升,在第13天恢復(fù)至對(duì)照組水平。同時(shí),苯肼注射后,從第1天開始小鼠外周血網(wǎng)織紅細(xì)胞總數(shù)和比例進(jìn)行性上升,低劑量組網(wǎng)織紅細(xì)胞總數(shù)和比例均于第6天達(dá)到最高,高劑量組網(wǎng)織紅細(xì)胞總數(shù)于第5天達(dá)到最高,網(wǎng)織紅細(xì)胞比例于第4天達(dá)到最高,之后便逐漸回落,于第13天完全恢復(fù)至正常水平。結(jié)論單次腹腔注射苯肼可誘發(fā)昆明小鼠輕度溶血性貧血,并刺激造血組織紅系造血以恢復(fù)外周血紅細(xì)胞水平,檢測外周血紅細(xì)胞總數(shù)和網(wǎng)織紅細(xì)胞總數(shù)可以反映該溶血性貧血模型的貧血進(jìn)展過程和紅系造血功能狀態(tài)。
[Abstract]:Objective to observe the changes of mature erythrocytes and reticulocytes in peripheral blood of Kunming mice after intraperitoneal injection of phenylhydrazine and to establish hemolytic anemia model of Kunming mice. Methods 18 healthy male Kunming mice were randomly divided into three groups: solvent control group, low dose phenylhydrazine group and high dose phenylhydrazine group. Rats in the control group were intraperitoneally injected with normal saline on the 0 th day. The animals in the low dose phenylhydrazine group and the high dose phenylhydrazine group were intraperitoneally injected with phenylhydrazine for 30 and 60 mg/kg bw, respectively. After that, erythrocyte count and reticulocyte count were collected from the tail of the rat every day. Results after the injection of phenylhydrazine, the total number of erythrocytes in peripheral blood and mature erythrocytes in the low dose phenylhydrazine group and the high dose phenylhydrazine group decreased gradually, and reached the lowest on the 5th and 4th day, respectively. The decrease of high dose phenylhydrazine group was significantly higher than that of low dose phenylhydrazine group, and after that, the erythrocyte count and mature erythrocyte count began to rise gradually, and returned to the control level on the 13th day. At the same time, the total number and proportion of reticulocyte in peripheral blood of mice gradually increased from the first day after injection of phenylhydrazine, and the total and proportion of reticulocyte in low dose group reached the highest level on the 6th day. The total number of high dose reticulocyte reached the highest on the 5th day, the reticulocyte ratio reached the highest on the 4th day, then gradually fell back to normal level on the 13th day. Conclusion A single intraperitoneal injection of phenylhydrazine can induce mild hemolytic anemia in Kunming mice and stimulate hematopoietic tissue erythropoiesis in order to restore the level of peripheral blood red blood cells. The total number of red blood cells and reticulocytes in peripheral blood can reflect the progression of anemia and the hemopoietic function of the hemolytic anemia model.
【作者單位】： 北京航空航天大學(xué)生物與醫(yī)學(xué)工程學(xué)院;
【基金】：國家自然科學(xué)基金(81573192;81072325)
【分類號(hào)】：R-332;R556

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