【摘要】：目的建立基于活體成像的裸鼠卵巢癌休眠模型并利用小動物活體成像技術(shù)驗證休眠細胞的存在。方法采用慢病毒載體對人卵巢癌skov-3細胞穩(wěn)定轉(zhuǎn)染螢火蟲熒光素酶基因(firefly luciferase gene),經(jīng)篩選獲得穩(wěn)定表達熒光素酶基因的人卵巢癌細胞株(skov-3/luc)。將skov-3/luc細胞接種至裸小鼠右側(cè)腋部皮下,成瘤后觀測熒光并做腫瘤切除手術(shù),術(shù)后的裸鼠再常規(guī)喂養(yǎng)8周后選取接種腫瘤處肉眼未見腫瘤組織生長的裸鼠進行活體成像觀察,顯示接種處腫瘤細胞熒光陽性者,即認為卵巢癌裸鼠休眠模型建立成功。結(jié)果篩選到了穩(wěn)定表達Luc的skov-3/luc細胞株;成功構(gòu)建了基于活體成像的裸鼠卵巢癌休眠模型,并利用小動物活體成像技術(shù)驗證了裸鼠體內(nèi)卵巢癌休眠細胞的存在。結(jié)論利用活體成像技術(shù)構(gòu)建的裸鼠卵巢癌休眠模型為研究腫瘤休眠的機制及誘導和維持腫瘤細胞休眠的方法提供了理想的實驗動物模型。
[Abstract]:Objective to establish a dormant model of ovarian cancer in nude mice based on in vivo imaging and to verify the existence of dormant cells by small animal imaging in vivo. Methods Human ovarian cancer cell line (skov-3/luc) with stable expression of luciferase gene was obtained by using lentivirus vector and stable transfection of luciferase gene (firefly luciferase gene), into human ovarian cancer skov-3 cells. Skov-3/luc cells were inoculated into the right axillary subcutaneous of nude mice. Fluorescence was observed after tumorigenesis and tumor resection was performed. After 8 weeks of routine feeding, nude mice inoculated with the tumor were selected to observe the growth of tumor tissue in vivo. Those who showed positive fluorescence of tumor cells at inoculation were considered to be successful in establishing dormancy model of ovarian cancer in nude mice. Results the skov-3/luc cell lines expressing Luc stably were screened, and the dormancy model of ovarian cancer in nude mice was successfully constructed based on in vivo imaging, and the existence of ovarian cancer dormant cells in nude mice was verified by small animal imaging in vivo. Conclusion\
【作者單位】： 廣東省中醫(yī)院婦科;廣州中醫(yī)藥大學第二臨床醫(yī)學院;
【基金】：國家自然科學基金資助項目(編號:81403422)
【分類號】：R-332;R737.31
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本文編號：2240601