【摘要】：甲型流感病毒是一種由脂質(zhì)分子包覆的球狀病毒,在病毒的表面分布有血凝素(hemagglutinin,簡稱HA蛋白)和神經(jīng)氨酸酶(neuraminidase,簡稱NA蛋白)兩種蛋白質(zhì)。一般認為,在流感病毒進入宿主細胞時,HA蛋白的功能是與細胞表面的唾液酸連接,使病毒粘連在宿主表面并隨著內(nèi)吞作用進入宿主體內(nèi),在完成新病毒繁殖后,NA蛋白則負責將新病毒從宿主細胞釋放。近年來一些實驗現(xiàn)象表明,在病毒進入宿主的過程中,NA蛋白也會影響HA蛋白功能的發(fā)揮,但是由于方法的限制,還沒有學者對這兩種蛋白之間的相互作用進行深入研究。 本文使用距離矩陣和相關(guān)性系數(shù),對HA蛋白和NA蛋白的進化過程進行了比較,發(fā)現(xiàn)二者具有較高的相關(guān)性,這說明HA蛋白和NA蛋白在功能上存在相互作用。隨后,本文將HA蛋白和NA蛋白的序列進行劃分,使用相同的方法計算各個片段之間在進化過程中的相關(guān)性,實現(xiàn)對兩蛋白相互作用位點的搜索。最后,本文應用所找到的位點,對HA蛋白和NA蛋白發(fā)生相互作用的過程進行了分子動力學模擬,并計算了兩個蛋白質(zhì)在接觸界面上的結(jié)合能,與實際情況下的疫情觀測結(jié)果進行比較,發(fā)現(xiàn)兩者相符,證明預測的結(jié)果是可信的。
[Abstract]:Influenza A virus is a globular virus coated with lipids. There are two proteins on the surface of influenza A virus: hemagglutinin, (HA protein) and neuraminidase (neuraminidase, protein NA protein). It is generally believed that the function of HA protein when influenza virus enters the host cell is to connect with sialic acid on the cell surface, so that the virus adheres to the host surface and enters the host body with endocytosis. The na protein is responsible for releasing the new virus from the host cells after the reproduction of the new virus. In recent years, some experimental phenomena have shown that the HA protein can also affect the function of the HA protein in the process of virus entering the host. However, due to the limitation of the methods, no further research has been done on the interaction between the two proteins. The evolutionary process of HA protein and NA protein were compared by using distance matrix and correlation coefficient. It was found that there was a high correlation between HA protein and NA protein, which indicated that there was a functional interaction between HA protein and NA protein. Then, the sequence of HA protein and NA protein were divided, and the correlation between each fragment in evolution was calculated by the same method, and the interaction sites of the two proteins were searched. Finally, the interaction between HA protein and NA protein was simulated by molecular dynamics, and the binding energy of the two proteins at the contact interface was calculated. The comparison with the observed results of epidemic situation shows that the predicted results are reliable.
【學位授予單位】：暨南大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R373

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