【摘要】：目的模擬臨床分割放療下的腮腺放射損傷,建立大鼠腮腺放射損傷后的放射性病理模型,為腮腺放射損傷的相關(guān)研究奠定基礎(chǔ)。方法選取成年Wistar大鼠20只,隨即分為兩組；實(shí)驗(yàn)組15只使用直線加速器照射,每次5Gy,共40Gy。對(duì)照組5只不做放射,正常飼養(yǎng)。觀察兩組實(shí)驗(yàn)動(dòng)物的飲食體重及腮腺區(qū)局部變化；照射完成4周后處死動(dòng)物,并在光鏡觀察腮腺組織病理改變。結(jié)果放射后實(shí)驗(yàn)大鼠均未出現(xiàn)可評(píng)價(jià)的異常神經(jīng)行為及體征；腺體組織結(jié)構(gòu)疏松,腺小葉間隙擴(kuò)大；間質(zhì)纖維組織增多,血管擴(kuò)張,片狀炎細(xì)胞浸潤(rùn)；以漿細(xì)胞為主,可見(jiàn)淋巴細(xì)胞；胞漿濃縮,染色深,核縮小,深染；腮腺內(nèi)毛細(xì)血管內(nèi)皮增厚,血管腫脹,周圍組織有明顯纖維化；血管內(nèi)皮增生,細(xì)胞呈梭形,向血管腔內(nèi)突出。實(shí)驗(yàn)組毛細(xì)血管密度明顯低于對(duì)照組。結(jié)論本實(shí)驗(yàn)條件下大鼠直線加速器分割照射腮腺,共40Gy,造成了較為典型的唾液腺放射損傷表現(xiàn),成功的模擬頭頸部放療所致的臨床及組織病理學(xué)變化,可作為唾液腺放療損傷模型。
[Abstract]:Objective to simulate the radiation injury of parotid gland induced by clinical fractionation radiotherapy and to establish the radiation-pathological model of parotid gland radiation injury in rats, so as to lay a foundation for the study of radiation injury of parotid gland. Methods Twenty adult Wistar rats were randomly divided into two groups: the experimental group (n = 15) was irradiated with a linear accelerator at a dose of 5 Gy for a total of 40 Gy. 5 rats in the control group were fed normally without radiation. The weight of diet and local changes of parotid gland were observed in the two groups, and the pathological changes of parotid tissue were observed under light microscope after 4 weeks of irradiation. Results No evaluable abnormal neurobehavioral and physical signs were found in the experimental rats after radiation. The glandular structure was loose, the glandular lobular space was enlarged, the interstitial fibrous tissue was increased, vasodilation and inflammatory cells were infiltrated, and plasma cells were the main. Visible lymphocytes; cytoplasm concentration, deep staining, nuclear shrinking, deep staining; parotid gland capillary endothelial thickening, vascular swelling, obvious fibrosis in surrounding tissues; vascular endothelial proliferation, cells spindle shape, protruding into the vascular lumen. The capillary density in the experimental group was significantly lower than that in the control group. Conclusion in this experiment, the parotid gland was irradiated by linear accelerator fractionation with 40 Gy, which resulted in the typical radiation injury of salivary gland. The clinical and histopathological changes of the parotid gland induced by simulated head and neck radiotherapy were successful. It can be used as a model of salivary gland radiation injury.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R-332

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