【摘要】：目的研究培元解郁方的抗抑郁作用和作用機(jī)制。方法采用利血平2.5 mg/kg腹腔注射,建立小鼠利血平抑郁模型;采用強(qiáng)迫游泳方法,建立急性應(yīng)激小鼠抑郁模型;采用長(zhǎng)期(14 d)束縛加噪聲,建立慢性應(yīng)激小鼠抑郁模型。觀察培元解郁方對(duì)利血平抑郁模型小鼠的體溫、眼瞼下垂、出圈率的影響;對(duì)急性應(yīng)激模型、慢性應(yīng)激模型小鼠的強(qiáng)迫游泳不動(dòng)時(shí)間的影響。采用酶聯(lián)免疫吸附(ELISA)法檢測(cè)急性應(yīng)激模型小鼠腦中5-羥色胺(5-HT)、去甲腎上腺素(NA)及吲哚胺-2,3-雙加氧酶(IDO)的含量;慢性應(yīng)激模型小鼠腦中5-HT、IDO的含量。并采用熒光定量PCR檢測(cè)慢性應(yīng)急模型小鼠腦中IDO mRNA的表達(dá)情況。結(jié)果培元解郁方各劑量組均能拮抗利血平抑郁模型小鼠的體溫下降(P0.01),此外高劑量還可改善利血平引起的眼瞼下垂、運(yùn)動(dòng)不能的狀況(P0.05),中劑量組可改善運(yùn)動(dòng)不能的狀況(P0.05)。培元解郁方中、低劑量組均可縮短急性應(yīng)激模型小鼠不動(dòng)時(shí)間(P0.05、P0.01),且顯著增加腦中5-HT、NA的含量(P0.05、P0.01)。培元解郁方高、中、低劑量組均可不同程度地縮短慢性應(yīng)激模型小鼠強(qiáng)迫游泳不動(dòng)時(shí)間、增加腦組織中5-HT的含量、降低IDO的含量并上調(diào)IDO mRNA的表達(dá)(P0.05)。結(jié)論培元解郁方對(duì)利血平、強(qiáng)迫游泳、慢性應(yīng)激造成的小鼠抑郁癥狀均有一定的改善作用。其機(jī)制可能與增加抑郁模型小鼠腦中單胺類神經(jīng)遞質(zhì)5-HT、NA含量,抑制IDO活性、下調(diào)IDO mRNA表達(dá)有關(guān)。
[Abstract]:Objective to study the antidepressant effect and mechanism of Peiyuan Jieyu prescription. Methods mice model of depression was established by intraperitoneal injection of reserpine 2.5 mg/kg, depression model of mice under acute stress was established by forced swimming, depression model of mice under chronic stress was established by long-term (14 d) restraint plus noise. To observe the effect of Peiyuan Jieyu recipe on body temperature, blepharoptosis and loop rate of reserpine depression model mice, and on the immobility time of forced swimming in acute stress model and chronic stress model mice. The contents of 5-hydroxytryptamine (5-HT), norepinephrine (NA) and indoleamine 3-dioxygenase (IDO) in brain of acute stress model mice were detected by enzyme-linked immunosorbent assay (ELISA). Fluorescence quantitative PCR was used to detect the expression of IDO mRNA in the brain of chronic emergency model mice. Results Peiyuan Jieyu prescription could antagonize the hypothermia of reserpine depression model mice (P0.01). In addition, high dose could also improve the condition of eyelid droop and motor failure induced by reserpine (P0.05), while the middle dose group could improve the condition of motility (P0.05). Peiyuan Jieyu recipe could shorten the immobility time (P0.05U P0.01) and significantly increase the content of 5-HTNNA in the brain (P0.05U P0.01) in the low dose group. Peiyuan Jieyu prescription could shorten the immobility time of forced swimming in chronic stress model mice, increase the content of 5-HT in brain tissue, decrease the content of IDO and up-regulate the expression of IDO mRNA (P0.05). Conclusion Peiyuan Jieyu prescription can ameliorate the depression symptoms of mice caused by reserpine, forced swimming and chronic stress. The mechanism may be related to the increase of monoamine neurotransmitter 5-HT na, the inhibition of IDO activity and the down-regulation of IDO mRNA expression in the brain of depression model mice.
【作者單位】： 北京中醫(yī)藥大學(xué)中藥學(xué)院;北京中醫(yī)藥大學(xué)中醫(yī)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(No.81373584)~~
【分類號(hào)】：R285.5;R-332

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