【摘要】：目的研究基因分型診斷在腸道病毒性中樞神經(jīng)系統(tǒng)感染病原學診斷的臨床應(yīng)用價值。 方法選取我院2006-06-2009-08期間診斷為病毒性腦炎的住院患兒共50例,均于入院的當天抽取腦脊液。試驗先經(jīng)種屬特異性引物擴增,篩選出腸道病毒樣本,然后分別用測序VP1及VP2序列的方法進行分型。試驗同時設(shè)立陽性對照及陰性對照。 結(jié)果50例臨床樣本中,共有45例臨床樣本檢測出EV RNA。其中,經(jīng)VP1序列分型方法可成功擴增分型38例,反應(yīng)產(chǎn)物為357 bp的片段。經(jīng)VP2序列分型方法可成功擴增分型34例,擴增產(chǎn)物為584 bp的片段。經(jīng)BLAST比對結(jié)果顯示：兩者在分型結(jié)果上有較高的一致性及互補性。 結(jié)論①經(jīng)VP1序列分型方法可以成功檢測臨床常見腸道病毒中的大多數(shù)血清型。②聯(lián)合VP2序列分型方法較單用VP1序列分型方法,分型率及敏感性更高,值得臨床上進一步推廣。
[Abstract]:Objective to study the clinical value of genotyping in etiological diagnosis of enterovirus central nervous system infection. Methods 50 hospitalized children with viral encephalitis diagnosed in our hospital from June 2006 to August 2009-08 were selected and cerebrospinal fluid (CSF) was collected on the day of admission. The samples of enterovirus were screened by species-specific primers, and then the VP1 and VP2 sequences were sequenced. Both positive and negative controls were established in the experiment. Results EV RNA. was detected in 45 of 50 clinical samples. Among them, 38 cases were successfully amplified by VP1 sequence typing, and the reaction product was 357 bp fragment. 34 cases were successfully amplified by VP2 sequence typing, and the amplified product was 584 bp. The results of BLAST comparison showed that there was a high consistency and complementarity in the typing results between the two groups. Conclusion (1) the VP1 sequence typing method can successfully detect most serotypes of enterovirus combined with VP2 sequence typing method, and the typing rate and sensitivity are higher than that of single VP1 sequence typing method, which is worthy of further clinical promotion.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R373.2

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本文編號：2215365