發(fā)布時間：2018-08-30 15:05

【摘要】：目的:探討蠅蛆肽對衰老小鼠的作用及其機(jī)制。方法:將60只小鼠隨機(jī)分為6組,除空白組外,其余五組用D-半乳糖致衰老,分為衰老模型組、維生素C組以及蠅蛆肽低、中、高劑量組(2、4、8 g/kg)。測定小鼠的脾臟和胸腺指數(shù)。采用羥胺法測定小鼠肝臟和腦及血清中的超氧化物歧化酶(SOD)活力,可見光法測定過氧化氫酶(CAT)和谷胱甘肽過氧化物酶(GSH-Px)的活力,以硫代巴比妥酸(TBA)法測定丙二醛(MDA)的含量。結(jié)果:與空白組相比,模型組小鼠脾指數(shù)、胸腺指數(shù)及肝臟指數(shù)均有顯著降低(p0.05),蠅蛆肽低、中、高劑量組臟器指數(shù)顯著增高(p0.05)。與空白組比較,模型組肝、腦中SOD、CAT、GSH-Px均降低,且MDA含量增高(p0.05),血清中SOD、CAT降低、MDA含量增高(p0.05);與模型組比較,蠅蛆肽低、中、高劑量組肝、腦組織中SOD、CAT、GSH-Px均顯著增高、MDA含量顯著降低(p0.05),血清中SOD、CAT顯著增高、MDA含量顯著降低(p0.05)。結(jié)論:蠅蛆肽通過改變衰老小鼠的臟器指數(shù)而對其產(chǎn)生免疫調(diào)節(jié)作用,可顯著提高小鼠肝臟和腦組織中的SOD、CAT、GSH-Px的活力,降低MDA的含量(p0.05),從而改變機(jī)體抗氧化能力。蠅蛆肽對衰老小鼠的影響機(jī)制可能與增強(qiáng)其體內(nèi)抗氧化酶活力及改變臟器指數(shù)相關(guān)。
[Abstract]:Objective: to investigate the effect and mechanism of fly maggot peptide on aging mice. Methods: sixty mice were randomly divided into 6 groups. Except the blank group, the other five groups were divided into aging model group, vitamin C group, low, medium and high dose group (2: 48 g/kg) with D-galactose. Spleen and thymus index of mice were measured. The activity of superoxide dismutase (SOD) in liver, brain and serum of mice was determined by hydroxylamine method, the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) were measured by visible light method, and the content of malondialdehyde (MDA) was determined by thiobarbituric acid (TBA) method. Results: compared with the blank group, the spleen index, thymus index and liver index of the model group were significantly decreased (p0.05), and the organ index of the middle and high dose groups were significantly higher than that of the control group (p0.05). Compared with the blank group, the content of SOD,CAT,GSH-Px in liver and brain of model group was decreased, and the content of MDA was increased (p0.05), and the content of SOD,CAT in serum was decreased (p0.05), compared with the model group, the content of maggot peptide was lower in model group, lower in middle dose group, and higher in high dose group. The content of SOD,CAT,GSH-Px in brain tissue was significantly higher than that in control group (p0. 05). The content of SOD,CAT in serum was significantly higher than that in control group (p0. 05). Conclusion: maggot peptide can significantly increase the activity of SOD,CAT,GSH-Px in liver and brain and decrease the content of MDA by changing the viscera index of aging mice (p0.05), which can change the antioxidant ability of the body. The mechanism of the effect of fly maggot peptide on aging mice may be related to the enhancement of antioxidant enzyme activity and the change of organ index.
【作者單位】： 貴州醫(yī)科大學(xué);
【基金】：貴州省2014年大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計劃項目(201410660019) 教育部2016年高校大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計劃項目(201610660310)
【分類號】：R339.38

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