【摘要】：目的對細(xì)菌間納米管形成機(jī)制及組成成分進(jìn)行探索,為預(yù)防細(xì)菌耐藥提供潛在靶標(biāo)。方法利用掃描電子顯微鏡觀察細(xì)菌間納米管的形成,用表面解吸常壓化學(xué)電離質(zhì)譜(SDAPCI-MS)技術(shù)和酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA)分別檢測細(xì)菌間納米管形成前后谷氨酸(glutamic,Glu)和肽聚糖(peptidoglycan,PGN)的濃度。結(jié)果相同菌量的金黃色葡萄球菌和大腸埃希菌在納米管形成后PGN含量明顯增多,且它們在納米管形成后消耗了更多的Glu。結(jié)論 PGN是納米管組成成分,Glu是促進(jìn)細(xì)菌間納米管形成的主要能量來源。
[Abstract]:Objective to explore the formation mechanism and components of bacterial nanotubes and to provide a potential target for the prevention of bacterial drug resistance. Methods the formation of interbacterial nanotubes was observed by scanning electron microscope (SEM). The concentrations of glutamate (glutamic,Glu) and peptidoglycan (peptidoglycan,PGN) before and after the formation of interbacterial nanotubes were detected by surface desorption chemical ionization mass spectrometry (SDAPCI-MS) and enzyme linked immunosorbent assay (ELISA). Results the PGN content of Staphylococcus aureus and Escherichia coli increased significantly after nanotube formation, and they consumed more Glu. after nanotube formation. Conclusion PGN is the main energy source to promote the formation of nanotubes.
【作者單位】： 南昌大學(xué)第二附屬醫(yī)院檢驗(yàn)科;南昌大學(xué)第一附屬醫(yī)院檢驗(yàn)科;
【基金】：江西省青年自然基金資助項(xiàng)目(No.20171BAB2150798) 江西省自然基金資助項(xiàng)目(No.20133BAB205062)聯(lián)合資助~~
【分類號】：R378

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