【摘要】：目的：本此實驗通過給大鼠喂高碘水,建立高碘大鼠模型,通過檢測同型半胱氨酸(HCY)、丙二醛(MDA)、抗氧化指標(biāo)(超氧化物歧化酶(SOD)、谷胱甘肽過氧化物酶(GSH-Px)指標(biāo),觀察不同濃度的高碘對氧化損傷的影響,并探討氧化損傷的機制。 方法： 1.實驗動物與分組：將32只斷乳1個月左右遠(yuǎn)交封閉群的SPF／VAF級wistar大鼠隨機分為4組,分別為正常對照(NC,水碘含量為5μg／L)組和10倍碘(10HI,2000μg／L)組、50倍碘(50HI,10000μg／L)組、100倍碘(100HI,20000μg／L)組,每組8只,雌雄各半。 2.記錄大鼠體重數(shù)據(jù)：采用自由飲水方式進行染毒,連續(xù)喂養(yǎng)6個月。 3.測定指標(biāo)：染毒結(jié)束后,測定血清同型半胱氨酸、丙二醛、超氧化物歧化酶(SOD)和谷胱甘肽過氧化物酶(GSH-Px)含量。數(shù)據(jù)采用SPSS16.0進行統(tǒng)計學(xué)分析。 [結(jié)果] 1.大鼠體重的檢測結(jié)果說明,無論雄性還是雌性,不同高碘組的大鼠體重與NC組大鼠對照均略有下降,但這個差異均沒有統(tǒng)計學(xué)意義。并且不同性別的大鼠體重之間對照,這個差異也沒有統(tǒng)計學(xué)意義。 2.大鼠血清中,HCY和MDA含量的檢測結(jié)果說明：與NC組對照,無論雄性還是雌性,50、100HI組的大鼠血清中HCY和MDA含量均較高,差異有統(tǒng)計學(xué)意義(P0.05或P0.01)。而不同性別大鼠血清中HCY和MDA含量間比較,差異無統(tǒng)計學(xué)意義。 3.大鼠血清中超氧化物歧化酶(SOD)和谷胱甘肽過氧化物酶(GSH-Px)測定結(jié)果表明,50、100HI組大鼠血清中SOD和GSH-Px含量均降低,差異有統(tǒng)計學(xué)意義(P0.05或P0.01)。而不同性別大鼠血清中HCY和MDA含量間比較,差異無統(tǒng)計學(xué)意義。 [結(jié)論] 1.無論是雄性還是雌性,不同高碘組的大鼠體重與NC組大鼠相比較均略有下降,但這差異都沒有統(tǒng)計學(xué)意義。并且不同性別的大鼠體重間比較,這差異也沒有統(tǒng)計學(xué)意義。 2.10倍高碘對大鼠血清中CY、MDA、SOD、GSH-Px未有影響,而50、100倍高碘可引起大鼠血清中HCY及MDA含量上升,SOD、GSH-Px含量下降。提示高碘可導(dǎo)致發(fā)生氧化反應(yīng)增強,抗氧化減弱,血管內(nèi)皮收到損傷。 3.不同性別大鼠血清中HCY、MDA、SOD、GSH-Px含量間比較,差異無統(tǒng)計學(xué)意義。
[Abstract]:Objective: to establish a rat model of hyperiodized rat by feeding high iodine water, and to detect the (SOD), glutathione peroxidase (GSH-Px) index of homocysteine (HCY), malondialdehyde (MDA),). The effects of different concentrations of iodine on oxidative damage were observed and the mechanism of oxidative damage was discussed. Methods: 1. Experimental animals and groups: 32 SPF/VAF wistar rats were randomly divided into 4 groups. They were normal control (5 渭 g / L in NC, water) and 10 times iodine (10 ~ 2 000 渭 g / L) groups with 50 times iodine (10 ~ 10 000 渭 g / L) and 100 ~ 100 times iodine (~ 20 000 渭 g / L) in each group, 8 rats in each group, female and male were half of each group. The rats' body weight data were recorded: exposed to free drinking water and fed continuously for 6 months. The content of homocysteine malondialdehyde (MDA) superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. The data were analyzed by SPSS16.0. [result] 1. The results showed that the weight of rats in different iodine groups was slightly lower than that in NC group, but the difference was not statistically significant. And there was no significant difference in body weight between different genders. 2. 2. The results showed that the content of HCY and MDA in serum of male and female rats was higher than that of NC group (P0.05 or P0.01). However, there was no significant difference in serum HCY and MDA levels between male and female rats. The results of serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) assay showed that the contents of SOD and GSH-Px in the serum of rats treated with X50100HI decreased significantly (P0.05 or P0.01). However, there was no significant difference in serum HCY and MDA levels between different sex rats. [conclusion] 1. No matter male or female, the weight of rats in different iodine groups was slightly lower than that in NC group, but the difference was not statistically significant. There was no significant difference in body weight between rats of different genders. 2.10 times excess iodine had no effect on serum CY,MDA,SOD,GSH-Px, while 50100 times iodine could increase the contents of HCY and MDA in serum and decrease the content of GSH-Px in serum of rats. These results suggest that excessive iodine can lead to increased oxidation reaction, decreased oxidation resistance and injury of vascular endothelium. There was no significant difference in serum HCY,MDA,SOD,GSH-Px content between different sex rats.
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R363

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