【摘要】：近年來隨著非藥物療法與保健的興起,磁療因具有操作簡單、無創(chuàng)無痛的特點,而越來越受到人們的關(guān)注,對磁場生物效應(yīng)的報道也越來越多。 但是目前磁療基礎(chǔ)研究薄弱,臨床上無嚴(yán)格使用規(guī)范,磁對生物體作用的量效關(guān)系和安全性研究尚不夠深入,磁對生物體作用的內(nèi)在機(jī)制不清楚。本研究在對磁療用永磁磁源空間磁場分析的基礎(chǔ)上,對正常培養(yǎng)和缺氧培養(yǎng)條件下的成纖維細(xì)胞受磁場影響的結(jié)果進(jìn)行研究,分析了磁場對成纖維細(xì)胞作用的量效關(guān)系,探討了磁場對生物體的作用機(jī)制。本文的主要研究內(nèi)容包括: 1.分析磁療用圓片永磁磁源的空間磁感強(qiáng)度分布,設(shè)計體外細(xì)胞培養(yǎng)加磁方法,準(zhǔn)確掌握磁場作用于細(xì)胞的磁感強(qiáng)度。 2.通過MTT法檢測細(xì)胞的活性,研究磁場對成纖維細(xì)胞增殖活性的影響。實驗發(fā)現(xiàn),選取的不同磁感強(qiáng)度磁場均不同程度上提高了成纖維細(xì)胞的活性。 3.通過測細(xì)胞培養(yǎng)液上清中LDH的含量,研究磁場對成纖維細(xì)胞膜損傷的影響。結(jié)果發(fā)現(xiàn),實驗中選取的大部分磁源對成纖維細(xì)胞膜是安全的。 4.通過測量細(xì)胞培養(yǎng)液上清中SOD與MDA的含量,研究了不同強(qiáng)度永磁磁場對細(xì)胞氧化損傷的影響。結(jié)果顯示為,細(xì)胞的抗氧化性經(jīng)過磁場的作用后被加強(qiáng)了。但氧化損傷的產(chǎn)物有多有少。 5.利用流式細(xì)胞儀檢測磁場對成纖維細(xì)胞凋亡的影響,從實驗結(jié)果來看,不同強(qiáng)度永磁磁場對成纖維細(xì)胞凋亡的影響顯現(xiàn)出了磁場對細(xì)胞的閾值作用。但大多磁場組對于成纖維細(xì)胞凋亡的影響并無規(guī)律可循。 本文研究不同磁感強(qiáng)度永磁磁源對成纖維細(xì)胞的影響,從細(xì)胞水平探討磁場的定量作用效果和量效關(guān)系,為規(guī)范永磁磁源的使用和磁作用效果的研究提供基礎(chǔ)性實驗依據(jù)。明確提出磁場的有效作用磁感強(qiáng)度量值或磁感強(qiáng)度量值范圍、以及磁場安全使用的磁感強(qiáng)度量值。
[Abstract]:In recent years, with the rise of non-drug therapy and health care, magnetic therapy has been paid more and more attention because of its simple operation, non-invasive and painless characteristics, and there are more and more reports on the biological effects of magnetic field. However, at present, the basic research of magnetic therapy is weak, there is no strict standard in clinical use, the study on the dose-effect relationship and safety of magnetic action on organism is not deep enough, and the internal mechanism of magnetic action on organism is not clear. Based on the spatial magnetic field analysis of permanent magnetic source for magnetic therapy, the effects of magnetic field on fibroblasts in normal and anoxic culture were studied, and the dose-effect relationship of magnetic field on fibroblasts was analyzed. The mechanism of magnetic field acting on organism is discussed. The main contents of this paper are as follows: 1. The spatial magnetic intensity distribution of magnetic disk magnetic source for magnetic therapy was analyzed, and the method of in vitro cell culture and magnetization was designed to accurately grasp the magnetic intensity of magnetic field acting on cells. 2. The effect of magnetic field on the proliferation of fibroblasts was studied by MTT assay. It was found that the activity of fibroblasts was increased by magnetic field with different intensity of magnetic inductance. The effect of magnetic field on the damage of fibroblast cell membrane was studied by measuring the content of LDH in the supernatant of cell culture medium. The results showed that most of the magnetic sources selected in the experiment were safe for fibroblast cell membrane. 4. 4. By measuring the content of SOD and MDA in the supernatant of cell culture medium, the effect of permanent magnetic field on cell oxidative damage was studied. The results showed that the antioxidant activity of cells was enhanced by magnetic field. But the products of oxidative damage are less. 5. The effect of magnetic field on the apoptosis of fibroblasts was detected by flow cytometry. From the experimental results, the effect of magnetic field on the apoptosis of fibroblasts showed the threshold effect of magnetic field. However, the effect of most magnetic field groups on fibroblast apoptosis is not regular. In this paper, the effects of permanent magnetic sources with different magnetic intensity on fibroblasts are studied, and the quantitative effect and dose-effect relationship of magnetic field are discussed from the cell level, which provides a basic experimental basis for standardizing the use of permanent magnetic sources and the study of magnetic effects. It is clear that the effective action of magnetic field or the range of magnetic inductance intensity, as well as the safe use of magnetic field.
【學(xué)位授予單位】：天津大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R35

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