【摘要】：目的通過建立大鼠慢性缺氧模型,觀察不同缺氧時(shí)間及應(yīng)用δ阿片受體激動劑下大鼠左皮層腦組織中Caspase-3的基因表達(dá)含量及相應(yīng)的蛋白含量變化。 方法將54只SD大鼠隨機(jī)分為3組,分為缺氧1天組,缺氧5天組,缺氧10天組,各組中又分為3個(gè)小組,即:對照組(C),缺氧組(H)及缺氧+給藥組(UFP-512)(H+D)。斷頭取腦后取得大鼠左皮層腦組織,以β-actin為內(nèi)參,利用RT-PCR及Western blotting實(shí)驗(yàn)測定所取組織中的Caspase-3的基因表達(dá)含量及相應(yīng)的蛋白含量,通過統(tǒng)計(jì)分析方法比較組內(nèi)指標(biāo)的差異性。 結(jié)果隨著缺氧時(shí)間的延長,組織中的Caspase-3基因表達(dá)含量及相應(yīng)的蛋白含量增加,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);缺氧1天組,Caspase-3基因表達(dá)含量及相應(yīng)的蛋白含量變化不明顯,組內(nèi)比較,差異無統(tǒng)計(jì)學(xué)意義(P0.05);缺氧5天組,缺氧組及缺氧+給藥組(UFP-512)的Caspase-3基因表達(dá)含量及相應(yīng)的蛋白含量與對照組兩兩比較,差異具有統(tǒng)計(jì)學(xué)意義(P0.05),并且缺氧+給藥組的Caspase-3基因表達(dá)含量及相應(yīng)的蛋白含量較缺氧組低,差異同樣具有統(tǒng)計(jì)學(xué)意義(P0.05);缺氧10天組的缺氧組及缺氧+給藥組(UFP-512)的Caspase-3基因表達(dá)含量及相應(yīng)的蛋白含量與對照組兩兩比較,差異具有統(tǒng)計(jì)學(xué)意義(P0.05),并且缺氧+給藥組的Caspase-3基因表達(dá)含量及相應(yīng)的蛋白含量與缺氧組比較,差異明顯,仍具有統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論Caspase-3可以作為慢性缺氧所致神經(jīng)元損傷程度的指標(biāo),而UFP-512作為一種新型的δ阿片受體激動劑通過激活δ阿片受體而減少Caspase-3的表達(dá),起到一定腦保護(hù)作用。
[Abstract]:Objective to establish a rat model of chronic hypoxia and to observe the changes of Caspase-3 gene expression and protein content in the left cortical brain of rats under different hypoxia time and 未 opioid receptor agonist (未 opioid receptor agonist). Methods Fifty-four SD rats were randomly divided into three groups: hypoxia for one day, hypoxia for 5 days and hypoxia for 10 days. Each group was divided into three groups: control group, (C), hypoxia group, (H) group and hypoxia administration group (UFP-512) (H D). The left cortical brain tissue of rats was obtained after decapitation. 尾 -actin was used as the internal reference. The Caspase-3 gene expression and the corresponding protein content were measured by RT-PCR and Western blotting experiments. The differences of the indexes in the group were compared by statistical analysis. Results with the prolongation of hypoxia time, the expression of Caspase-3 gene and the corresponding protein content in tissues increased significantly (P0.05), but the expression of Caspase-3 gene and the corresponding protein content in hypoxia group were not significantly changed after 1 day of hypoxia, but there was no significant difference in the expression of Caspase-3 gene and the corresponding protein content between the two groups. There was no significant difference (P0.05). The expression of Caspase-3 gene and protein in hypoxia group, hypoxia group and hypoxia administration group (UFP-512) were compared with those in control group. The difference was statistically significant (P0.05), and the expression of Caspase-3 gene and the corresponding protein content in hypoxia group were lower than those in hypoxia group. The difference was also statistically significant (P0.05). The expression of Caspase-3 gene and the corresponding protein content in hypoxia group and UFP-512 group were compared with those in control group. The difference was statistically significant (P0.05), and the expression of Caspase-3 gene and the corresponding protein content in hypoxia group were significantly higher than those in hypoxia group (P0.05). Conclusion Caspase-3 can be used as a marker of neuron damage induced by chronic hypoxia, and UFP-512, as a new type of 未 opioid receptor agonist, can reduce the expression of Caspase-3 by activating 未 opioid receptor, and play a protective role in brain.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R363

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