【摘要】：目的本實(shí)驗(yàn)研究通過(guò)建立不同鼠齡絕經(jīng)模型,觀(guān)察雌激素對(duì)絕經(jīng)后不同鼠齡大鼠血清一氧化氮(NO)含量、主動(dòng)脈內(nèi)皮型一氧化氮合酶(eNOS) mRNA表達(dá)的影響以及主動(dòng)脈組織形態(tài)學(xué)的變化。 方法將青年(3月齡)雌性wistar大鼠30只和老年(18-20月齡)雌性wistar大鼠30只分別隨機(jī)分為三組,每組10只：假手術(shù)組(Sham組)、去卵巢組(OVX組)、去卵巢+苯甲酸雌二醇補(bǔ)充組(OVE組)。Sham組：假手術(shù)+每3天肌注0.04m1生理鹽水；OVX組：去除雙側(cè)卵巢+每3天肌注0.04m1生理鹽水；OVE組：去除雙側(cè)卵巢+每3天肌注10ug苯甲酸雌二醇,4個(gè)月后處死大鼠并測(cè)定各組血清NO的水平、主動(dòng)脈eNOS mRNA的表達(dá)并觀(guān)察主動(dòng)脈的組織形態(tài)學(xué)變化。 結(jié)果1.青年Sham組,OVX組,OVE組的血清NO水平分別明顯高于老年Sham組,OVX組,OVE組的血清NO水平,p0.05,p0.05,p0.05。去卵巢后,青年大鼠OVX組的血清NO水平明顯低于Sham組,p0.05,但是在老年OVX組無(wú)明顯差異。補(bǔ)充雌激素后,青年、老年OVE組的血清NO水平較OVX組明顯增加,p0.05,p0.05。2.青年Sham組、OVE組主動(dòng)脈eNOS mRNA的表達(dá)分別高于老年Sham組、OVE組,p0.05,p0.05。但是青年OVX組與老年OVX組主動(dòng)脈eNOS mRNA的表達(dá)無(wú)明顯差異,p=0.462。去卵巢后青年OVX組主動(dòng)脈eNOS mRNA的表達(dá)明顯顯低于Sham組,p0.05；補(bǔ)充雌激素后,青年OVE組主動(dòng)脈eNOS mRNA的表達(dá)較OVX組明顯增加,p0.05。老年大鼠Sham組,OVX組,OVE組之間主動(dòng)脈eNOS mRNA的表達(dá)無(wú)明顯差異,p=0.579。 結(jié)論1.在青年絕經(jīng)后大鼠,雌激素治療后可能通過(guò)上調(diào)內(nèi)皮型一氧化氮合酶(eNOS) mRNA的表達(dá)來(lái)增加血清一氧化氮(NO)的水平。2.在老年絕經(jīng)大鼠,雌激素治療后增加血清一氧化氮(NO)的合成不是通過(guò)上調(diào)內(nèi)皮型一氧化氮合酶(eNOS) mRNA的表達(dá)來(lái)實(shí)現(xiàn)。3.隨著年齡增加,血清一氧化氮(NO)水平和內(nèi)皮型一氧化氮合酶(eNOS) mRNA的表達(dá)都逐漸減少。
[Abstract]:Objective to study the effect of estrogen on serum nitric oxide (NO) content in postmenopausal rats by establishing menopausal models of different ages. Effects of aortic endothelial nitric oxide synthase (eNOS) mRNA) expression and changes of aortic histomorphology. Methods Thirty young (3-month-old) female wistar rats and 30 aged (18-20 months) female wistar rats were randomly divided into three groups. There were 10 rats in each group: sham operation group (Sham group), ovariectomized group (OVX group), ovariectomized estradiol benzoate supplement group (OVE group) .Sham group: sham group was given 0.04m1 saline every 3 days; In OVE group, 10ug estradiol benzoate was injected intramuscularly every 3 days after removal of bilateral ovaries. After 4 months, the rats were killed and serum no levels were measured. The expression of eNOS mRNA in aorta and the histomorphologic changes of aorta were observed. Result 1. The serum no level of OVX group in young Sham group was significantly higher than that of OVX group in elderly Sham group. After ovariectomy, the serum no level in OVX group was significantly lower than that in Sham group (p 0.05), but there was no significant difference in aged OVX group. After estrogen supplementation, the serum no level in the young and elderly OVE group was significantly higher than that in the OVX group. The expression of aortic eNOS mRNA in the young Sham group was higher than that in the elderly Sham group. However, there was no significant difference in the expression of aortic eNOS mRNA between the young OVX group and the old OVX group. After ovariectomized, the expression of aortic eNOS mRNA in the young OVX group was significantly lower than that in the Sham group (p 0.05), and after estrogen supplementation, the expression of eNOS mRNA in the aorta of the young OVE group was significantly higher than that in the OVX group. There was no significant difference in the expression of eNOS mRNA in aorta between OVX group and OVX group in aged rats. Conclusion 1. In young postmenopausal rats, estrogen treatment may increase the level of serum nitric oxide (NO) by up-regulating the expression of endothelial nitric oxide synthase (eNOS) mRNA). In elderly postmenopausal rats, the increase in serum nitric oxide (NO) synthesis after estrogen therapy is not achieved by up-regulating the expression of endothelial nitric oxide synthase (eNOS) mRNA). The level of serum nitric oxide (NO) and the expression of endothelial nitric oxide synthase (eNOS) mRNA) decreased with age.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R-332

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