【摘要】：目的建立斑馬魚的急性缺氧模型,并對(duì)五環(huán)三萜類化合物的抗缺氧活性進(jìn)行初篩。方法 64只斑馬魚放置低氧操作箱中建立斑馬魚的急性缺氧模型,并隨機(jī)分成空白組、陽(yáng)性對(duì)照組[槲皮素(QT)]、積雪草酸(AA)組、羥基積雪草酸(MA)組、積雪草苷(AS)組、羥基積雪草苷(MS)組、熊果酸(UA)組、甘草次酸(GA)組,每組8只。實(shí)驗(yàn)組和陽(yáng)性對(duì)照組所加藥物的終濃度均為2.5μg·mL~(-1),空白組為加入相同劑量的20%PEG-400溶液。比較各組斑馬魚的生理狀態(tài)和半數(shù)死亡(4只)所用時(shí)間。結(jié)果斑馬魚的缺氧條件為20℃,3%的氧氣濃度。空白組、陽(yáng)性對(duì)照組、AA組、MA組、AS組、MS組、UA組、GA組的半數(shù)死亡所用時(shí)間分別為(126.22±4.92),(166.98±3.30),(157.02±4.62),(181.02±3.54),(118.98±3.78),(121.98±1.98),(127.98±2.82),(129.48±5.94)min,除AS組和GA組外,其他組斑馬魚半數(shù)死亡時(shí)間與空白組比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論用斑馬魚急性缺氧模型對(duì)潛在抗缺氧活性物質(zhì)的篩選評(píng)價(jià)建模容易、結(jié)果可靠;三萜類苷元(AA、MA、UA和GA)具有抗缺氧作用,且烏蘇烷型三萜(AA、MA和UA)的抗缺氧作用更明顯;三萜類皂苷(AS、MS)不具有抗缺氧活性。
[Abstract]:Objective to establish the acute hypoxia model of zebrafish and to screen the antihypoxia activity of pentacyclic triterpenoids. Methods the acute hypoxia model of zebrafish was established by placing 64 zebrafish in hypoxic box. The model was randomly divided into three groups: positive control group [quercetin (QT)], (AA) group, (MA) group and (AS) group. Hydroxy asiaticoside (MS) group, ursolic acid (UA) group, glycyrrhetinic acid (GA) group, 8 rats in each group. The final concentration of the drug was 2.5 渭 g mL ~ (-1) in the experimental group and the positive control group, and the same dose of 20%PEG-400 solution was added in the blank group. The physiological state of zebrafish in each group and the time of half death (4) were compared. Results the oxygen concentration of zebrafish was 3% at 20 鈩，

本文編號(hào)：2179124