【摘要】：目的:探究體外誘導(dǎo)造血干細(xì)胞向紅系分化過(guò)程中AB血清對(duì)終末期紅系分化進(jìn)程的影響。方法:從臍帶血中用免疫磁珠法分離提取CD34+細(xì)胞,體外誘導(dǎo)其向紅系分化。根據(jù)培養(yǎng)體系中是否添加AB血清,設(shè)置實(shí)驗(yàn)組為添加AB血清組,對(duì)照組為不添加AB血清組。通過(guò)檢測(cè)細(xì)胞表面紅系分化標(biāo)志分子糖化血紅蛋白A(glycophorin A,GPA)、人紅細(xì)胞陰離子交換蛋白Band3及整合素α4(α4-integrin)的表達(dá)反映終末紅系分化進(jìn)程,用流式細(xì)胞術(shù)檢測(cè)紅系細(xì)胞終末分化脫核情況。結(jié)果:與對(duì)照組相比,實(shí)驗(yàn)組GPA陽(yáng)性細(xì)胞百分率較高,Band3表達(dá)較高,而α4-integrin表達(dá)較低(P0.05)。實(shí)驗(yàn)組細(xì)胞脫核百分率明顯高于對(duì)照組(P0.05)。結(jié)論:在體外紅系發(fā)育培養(yǎng)體系中,AB血清對(duì)終末期紅系分化進(jìn)程有一定的促分化、促脫核作用。
[Abstract]:Aim: to investigate the effect of AB serum on the process of erythroid differentiation induced by hematopoietic stem cells in vitro. Methods: CD34 cells were isolated from umbilical cord blood by immunomagnetic beads and induced to differentiate into erythrocytes in vitro. According to whether the AB serum was added in the culture system, the experimental group was added AB serum group, and the control group was no AB serum group. The expression of human erythrocyte anion exchange protein (Band3) and integrin 偽 4 (偽 4-integrin) were detected to reflect the process of terminal erythroid differentiation. Flow cytometry was used to detect the denucleation of erythrocyte terminal differentiation. Results: compared with the control group, the percentage of GPA positive cells in the experimental group was higher than that in the control group, while the expression of 偽 4-integrin was lower (P0.05). The percentage of cell denucleation in the experimental group was significantly higher than that in the control group (P0.05). Conclusion: in vitro erythroid development culture system, the serum Asab can promote the differentiation and denucleation of the end-stage erythroid.
【作者單位】： 中南大學(xué)生命科學(xué)學(xué)院分子生物學(xué)研究中心;中南大學(xué)湘雅醫(yī)學(xué)院;中南大學(xué)湘雅醫(yī)院血液科;
【基金】：國(guó)家自然科學(xué)基金(81270576) 中南大學(xué)校級(jí)大學(xué)生創(chuàng)新訓(xùn)練計(jì)劃項(xiàng)目(YC13296)~~
【分類(lèi)號(hào)】：R329.2
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本文編號(hào)：2176729