發(fā)布時間：2018-08-01 16:41

【摘要】：[目的]研究致癌物質苯并(a)芘[Benzo(a)pyrene, B(a)P]對人支氣管上皮細胞線粒體損傷的影響。 [方法]實驗組分別用B(a)P濃度為50umol/1、1Oumol/1、1.Oumol/1的達爾伯克必需基本培養(yǎng)液(Dulbecco's Minimum Essential Medium,DMEM)對人支氣管上皮細胞BEAS-2B進行培養(yǎng),空白對照組用正常DMEM養(yǎng)液對人支氣管上皮細胞BEAS-2B進行培養(yǎng)。培養(yǎng)24小時、48小時、72小時后,流式細胞儀測定細胞線粒體膜電位的變化情況；逆轉錄-聚合酶鏈反應(Reversed-transcription PCR, RT-PCR)檢測線粒體細胞色素b(cytochrome b,Cyt-b)基因的表達情況。結果應用spss17.0統(tǒng)計軟件進行統(tǒng)計學處理。 [結果]1.細胞線粒體膜電位的變化隨培養(yǎng)時間延長,與空白對照組相比,實驗組細胞線粒體膜電位水平逐漸下降,但50umol/1B(a)P、10umol/1B(a)P組細胞線粒體膜電位水平下降趨勢更加明顯(P0.01) 2.線粒體Cyt-b基因的表達細胞培養(yǎng)24h后,50umol/1B(a)P、10umol/1B(a)P組細胞Cyt-b mRNA相對表達量較空白對照組升高,50umol/l B(a)P組差異具有統(tǒng)計學意義(P0.05),10umol/1B(a)P、1.0umol/1B(a)P組表達較空白對照組無明顯差異(P0.05)；細胞培養(yǎng)48h后,50umol/1B(a)P.10umol/1B(a)P組細胞Cyt-b mRNA相對表達量較空白對照組升高,差異具有顯著統(tǒng)計學意義(P0.01),1.0umol/1B(a)P組表達較空白對照組無明顯差異(P0.05)；細胞培養(yǎng)72h后,50umol/1B(a)P、10umol/1B(a)P組細胞Cyt-b mRNA相對表達量較空白對照組降低,50umol/1B(a)P組差異具有統(tǒng)計學意義(P0.05),1Oumol/1B(a)P、1.Oumol/1B(a)P組表達較空白對照組無明顯差異(P0.05)。 [結論]B(a)P對人支氣管上皮細胞BEAS-2B線粒體有損傷作用。B(a)P濃度越高,作用時間越長,損傷作用越強。
[Abstract]:[objective] to study the effect of benzo (a) pyrene [Benzo (a) pyrene, B (a) P] on mitochondrial damage in human bronchial epithelial cells. [methods] the human bronchial epithelial cells (BEAS-2B) were cultured in the experimental group with a concentration of 50 umoll / 1 Oumoll / 1 Oumoll / 1 Dalbek essential culture medium (Dulbecco's Minimum Essential Media DMEM), and the blank control group with the normal DMEM culture solution to culture the human bronchial epithelial cell BEAS-2B. After cultured for 24 hours and 48 hours for 72 hours, the changes of mitochondrial membrane potential and the expression of mitochondrial cytochrome b (cytochrome b cyt b gene were detected by flow cytometry and Reversed-transcription polymerase chain reaction (RT-PCR). Results spss17.0 statistical software was used for statistical processing. [result] 1. Compared with the blank control group, the level of mitochondrial membrane potential in the experimental group decreased gradually, but the decrease trend of the mitochondrial membrane potential in the 50umol/1B (a) 10 umol / 1B (a) P group was more obvious (P0.01) 2. Compared with the control group, the relative expression of Cyt-b mRNA in the mtDNA Cyt-b gene expression group was significantly higher than that in the control group (P0.05). There was no significant difference in the expression of Cyt-b mRNA between the 10 渭 mol / 1B (a) and 1.0 umolol / 1B (a) P group (P0.05). After 48 hours of cell culture, the relative expression of Cyt-b mRNA in 50 umol / 1B (a) P.10umol/1B (a) P group was higher than that in the blank control group, and the difference was statistically significant (P0.01). The expression of Cyt-b mRNA in 1.0 umol / 1B (a) P group was not significantly different from that in the blank control group (P0.05). After 72 hours of cell culture, there was no significant difference in the relative expression of Cyt-b mRNA between the control group and the control group (P0.05) compared with the control group (P0.05). The expression of Oumolol / 1B (a) / 1B (a) P in the Oumolr / 1B (a) P group was not significantly different from that in the blank control group (P0.05). [conclusion] B (a) P can damage BEAS-2B mitochondria of human bronchial epithelial cells. The higher the concentration of B (a) P is, the longer the time is and the stronger the damage is.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R363

【參考文獻】

相關期刊論文 前9條

1 王建炳;姜勇;喬友林;;肺癌危險因素人群歸因風險研究進展[J];癌癥進展;2011年05期

2 高國福,姚樹祥,孫秀娣,常潤生,姜勇,羅學昌,晉萍,喬友林;云南錫礦工肺癌的隊列研究[J];中國肺癌雜志;2002年02期

3 朱文,周清華;肺癌的遺傳易感性和環(huán)境致癌[J];中國肺癌雜志;2003年06期

4 朱佳杰;;肺癌發(fā)生相關基因的研究進展[J];醫(yī)藥論壇雜志;2011年07期

5 鞠方鶴;肖祖克;;肺癌腫瘤標志物的研究近況[J];南昌大學學報(醫(yī)學版);2010年02期

6 王黎明;鐘才高;張洪霞;關嵐;;香煙煙霧水溶性提取物對肝細胞線粒體的損傷[J];毒理學雜志;2008年02期

7 林珊;張宏;韓婷;吳錦忠;Khalid RAHMAN;秦路平;;紫花牡荊素體內抗炎作用的研究(英文)[J];中西醫(yī)結合學報;2007年05期

8 王海燕,蔡兵,崔承彬,張冬云,楊寶峰;蔓荊子活性成分vitexicarpin誘導K562細胞凋亡的機制[J];藥學學報;2005年01期

9 高緒芳;陳俊;李明川;文衛(wèi)華;馬曉軍;衡正昌;;苯并a芘致人k-ras基因DNA損傷位點檢測[J];中國公共衛(wèi)生;2009年08期

，

本文編號：2158193