【摘要】：目的探討丘腦腹內(nèi)側(cè)核高頻電刺激能否改善帕金森病模型大鼠前肢使用不對(duì)稱行為。方法將33只SD大鼠隨機(jī)分為實(shí)驗(yàn)組22只和對(duì)照組11只。利用立體定向的方法將刺激電極埋入右側(cè)丘腦腹內(nèi)側(cè)核,用于注射6-羥基多巴胺的套管埋入右側(cè)內(nèi)側(cè)前腦束。實(shí)驗(yàn)組大鼠右側(cè)內(nèi)側(cè)前腦束內(nèi)注射12μg的6-OHDA,對(duì)照組注射相同體積的生理鹽水。對(duì)造模前、造模后和給予DBS時(shí)的大鼠進(jìn)行前肢使用不對(duì)稱測(cè)試。黑質(zhì)酪氨酸羥化酶免疫組化染色檢測(cè)黑質(zhì)多巴胺能神經(jīng)元?dú)p程度。尼氏染色確定刺激電極尖端位置。結(jié)果從22只大鼠中獲得了15只成功的PD大鼠模型。與毀損前相比,模型大鼠毀損后的不對(duì)稱指數(shù)顯著增大(P=0.000)。給予高頻VM-DBS后,不對(duì)稱指數(shù)顯著下降,與毀損后未給予VM-DBS時(shí)比較,差異有統(tǒng)計(jì)學(xué)意義(P=0.009);而與毀損前相比,差異不顯著(P=0.214)。給予低頻VM-DBS后,不對(duì)稱指數(shù)無明顯改變,與毀損后未給予VM-DBS時(shí)比較,差異無統(tǒng)計(jì)學(xué)意義(P=0.4);而與毀損前相比,差異顯著(P=0.036)。結(jié)論丘腦腹內(nèi)側(cè)核高頻電刺激能夠改善6-羥基多巴胺偏側(cè)帕金森病模型大鼠的前肢使用不對(duì)稱行為,丘腦腹內(nèi)側(cè)核可能是帕金森病深部腦刺激治療的潛在有效靶核團(tuán)。
[Abstract]:Objective to investigate whether high frequency stimulation of ventromedial nucleus of thalamus can improve the asymmetric behavior of forelimb in Parkinson's disease model rats. Methods 33 SD rats were randomly divided into experimental group (n = 22) and control group (n = 11). The stimulation electrode was implanted into the ventromedial nucleus of the right thalamus by stereotactic method and the 6-hydroxydopamine cannula was injected into the right medial forebrain tract. The right medial forebrain of the experimental group was injected with 12 渭 g of 6-OHDAand the control group was injected with the same volume of normal saline. The antebrachial asymmetry test was performed on rats before, after and after DBS administration. The damage of substantia nigra dopaminergic neurons was detected by tyrosine hydroxylase immunohistochemical staining. The tip position of the stimulation electrode was determined by Nissl staining. Results Fifteen successful PD rat models were obtained from 22 rats. The asymmetry index of model rats increased significantly (P0. 000). After high frequency VM-DBS, the asymmetry index decreased significantly (P 0. 009), but there was no significant difference (P 0. 214). There was no significant change in asymmetry index after low frequency VM-DBS, but there was no significant difference (P0. 036) between the two groups when VM-DBS was not given after the damage (P0. 4), but significant difference was found between the two groups (P0. 036). Conclusion High frequency electrical stimulation of ventromedial nucleus of thalamus can improve the asymmetric behavior of the forelimb of 6-hydroxydopamine hemiparkinsonism rats. The ventromedial nucleus of thalamus may be a potentially effective target nucleus for deep brain stimulation in Parkinson's disease.
【作者單位】： 海南醫(yī)學(xué)院附屬醫(yī)院神經(jīng)外科;南方醫(yī)科大學(xué)珠江醫(yī)院神經(jīng)外科;青海大學(xué)附屬醫(yī)院神經(jīng)外科;中國科學(xué)院心理研究所;陸軍總醫(yī)院附屬八一腦科醫(yī)院;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(編號(hào):30872662) 海南醫(yī)學(xué)院科研培育基金項(xiàng)目(編號(hào):HY2015-19)
【分類號(hào)】：R742.5;R-332

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