【摘要】：目的探討黃芪百合顆粒對(duì)高原低氧模型小鼠腦組織氧化應(yīng)激損傷的影響。方法將60只SPF級(jí)昆明小鼠隨機(jī)分為對(duì)照組、模型組、黃芪百合顆粒低、中、高(1.75、3.5、7 mg·kg~(-1))劑量組。常規(guī)飼養(yǎng)3 d后,灌胃給藥,每日1次,連續(xù)17 d。從第14日起每日灌胃30 min后除對(duì)照組外其余各組小鼠于低氧艙中模擬高海拔低壓低氧環(huán)境進(jìn)行暴露,持續(xù)暴露3 d,眼球采血后處死小鼠。采用比色分析法測(cè)定腦組織中超氧化物歧化酶活性、丙二醛含量及總抗氧化能力,HE染色在顯微鏡下觀察腦組織的形態(tài)變化進(jìn)行病理評(píng)分并計(jì)數(shù)炎細(xì)胞數(shù)量。結(jié)果與對(duì)照組比較,模型組小鼠腦質(zhì)量明顯增加,超氧化物歧化酶活性和總抗氧化能力明顯降低,腦丙二醛含量、腦組織病理評(píng)分和炎細(xì)胞數(shù)量均明顯增加(P0.01)。與模型組比較,黃芪百合顆粒中、高劑量組小鼠的腦組織超氧化物歧化酶活性和總抗氧化能力均升高,丙二醛含量、病理評(píng)分及炎細(xì)胞數(shù)均降低(P0.05或P0.01)。結(jié)論黃芪百合顆粒對(duì)高原低壓低氧環(huán)境下小鼠腦損傷具有良好保護(hù)作用,可能是通過增強(qiáng)腦組織自由基清除能力實(shí)現(xiàn)的。
[Abstract]:Objective to investigate the effect of Huangqi lily granule on oxidative stress injury in brain tissue of high altitude hypoxia model mice. Methods Sixty Kunming mice of SPF grade were randomly divided into control group, model group, low, medium and high dose group (1.75 渭 g / kg ~ (-1). After 3 days of routine feeding, the drug was administered by gavage once a day for 17 days. The mice in each group were exposed to hypoxia-hypoxia environment at high altitude and low pressure and hypoxia for 30 min from the 14th day, and the mice were killed after eyeball blood collection for 3 days. The activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and the total antioxidant capacity of brain tissue were determined by colorimetric analysis. The morphological changes of brain tissue were observed under microscope and the number of inflammatory cells was counted. Results compared with the control group, the brain mass of the model group was significantly increased, the activity of superoxide dismutase (SOD) and the total antioxidant capacity were significantly decreased, the content of malondialdehyde (MDA) in the brain, the pathological score of brain tissue and the number of inflammatory cells were significantly increased (P0.01). Compared with the model group, the activity of superoxide dismutase (SOD) and the total antioxidant ability of the mice in high dose group were increased, the content of malondialdehyde (MDA), pathological score and the number of inflammatory cells were decreased (P0.05 or P0.01). Conclusion Astragalus lily granule has a good protective effect on brain injury in mice under high altitude low pressure and hypoxia, which may be achieved by enhancing the free radical scavenging ability of brain tissue.
【作者單位】： 甘肅省高校重大疾病分子醫(yī)學(xué)與中醫(yī)藥防治研究省級(jí)重點(diǎn)實(shí)驗(yàn)室;敦煌醫(yī)學(xué)與轉(zhuǎn)化省部共建教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：2012甘肅省科技支撐計(jì)劃項(xiàng)目,No.120FKCA169 隴原青年創(chuàng)新人才扶持計(jì)劃,No.2014-93-72
【分類號(hào)】：R285.5;R-332
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本文編號(hào)：2142474