【摘要】：目的探討紅樹林來源的淡紫擬青霉胞外多糖對小鼠骨髓源性樹突細(xì)胞(DCs)功能成熟的影響。方法從小鼠骨髓腔中分離獲得骨髓細(xì)胞,加入重組小鼠粒細(xì)胞-巨噬細(xì)胞集落刺激因子(rmGM-CSF)、重組小鼠白細(xì)胞介素-4(rmIL-4)誘導(dǎo)分化為未成熟DCs,用不同濃度淡紫擬青霉胞外多糖干預(yù),流式細(xì)胞術(shù)檢測DCs的表面標(biāo)志CD11c、主要組合相容性復(fù)合體(MHCⅡ)類分子、CD80、CD86的表達(dá)情況及吞噬葡聚糖(FITC-dextran)的能力,反轉(zhuǎn)錄-聚合酶鏈反應(yīng)(RT-PCR)檢測該多糖對DCs Toll樣受體(TLR)2mRNA和TLR4mRNA表達(dá)的影響。結(jié)果經(jīng)300、400μg/mL多糖作用48h后DCs表面分子CD11c、MHCⅡ類分子、CD80、CD86的表達(dá)較空白對照組顯著上調(diào)(P0.01);經(jīng)多糖作用的DCs吞噬FITC-dextran能力下降,尤其是300、400μg/mL的多糖與空白對照組相比作用效果明顯(P0.05);另外,該多糖還可下調(diào)DCs TLR2mRNA和TLR4mRNA的表達(dá),尤其經(jīng)100~400μg/mL多糖處理的DCs下調(diào)作用顯著,與空白對照組相比差異有統(tǒng)計學(xué)意義(P0.05)。結(jié)論淡紫擬青霉胞外多糖可上調(diào)小鼠骨髓源性未成熟DCs表面CD11c、MHCⅡ類分子、CD80和CD86的表達(dá),降低其吞噬能力,下調(diào)TLR2mRNA和TLR4mRNA的表達(dá),初步表明該多糖可刺激DCs分化成熟。
[Abstract]:Objective to investigate the effect of mangrove derived Penicillium lilicum extracellular polysaccharide on the functional maturation of mouse bone marrow derived dendritic cells (DCs). Methods bone marrow cells were isolated from the bone marrow cavity of mice, and the recombinant mouse granulocyte macrophage colony stimulating factor (rmGM-CSF) was added to the mice. The induced differentiation of interleukin -4 (rmIL-4) in the mice was induced into immature mice. DCs, using different concentrations of Paecilomyces lilici extracellular polysaccharide intervention, flow cytometry detection of the surface markers of DCs CD11c, the main combinatorial complex (MHC II) molecules, CD80, CD86 expression and the ability to phagocytic dextran (FITC-dextran), reverse transcription polymerase chain reaction (RT-PCR) detection of the polysaccharide on DCs Toll like receptor (TLR) 2mRNA and exports The effect of the expression of 4mRNA. Results the expression of DCs surface molecules CD11c, MHC II, CD80, CD86 was significantly up (P0.01) after the action of 300400 mu g/mL polysaccharide, CD80, CD86, and the FITC-dextran ability of the DCs was decreased, especially the 300400 micron g/mL polysaccharide was significantly more effective than the empty white control group. Polysaccharides could also reduce the expression of DCs TLR2mRNA and TLR4mRNA, especially the down-regulation of DCs treated with 100~400 mu g/mL polysaccharide, and there was a significant difference between the control group and the blank control group (P0.05). Conclusion PP can increase the CD11c, MHC II, CD80 and CD86. The ability to downregulate TLR2mRNA and TLR4mRNA expression indicated that the polysaccharide could stimulate DCs differentiation and maturation.
【作者單位】： 海南醫(yī)學(xué)院臨床學(xué)院;海南醫(yī)學(xué)院熱帶醫(yī)學(xué)與檢驗學(xué)院;
【基金】：國家自然科學(xué)基金資助項目(31260225) 海南省自然科學(xué)基金資助項目(813248) 海南醫(yī)學(xué)院科研培育基金資助項目(HY2014-015)
【分類號】：R392

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