本文選題：壞死性小腸結(jié)腸炎 + 雙歧桿菌�。� 參考：《第三軍醫(yī)大學(xué)學(xué)報(bào)》2016年05期

【摘要】：目的探討雙歧桿菌對(duì)壞死性小腸結(jié)腸炎(necrotizing enterocolitis,NEC)新生大鼠模型腸道通透性及血清Zonulin表達(dá)的影響,分析雙歧桿菌保護(hù)NEC可能的機(jī)制。方法將60只新生1 d的SD大鼠按隨機(jī)數(shù)字表法分為3組(n=20):對(duì)照組、壞死性小腸結(jié)腸炎新生大鼠組(NEC組)及雙歧桿菌干預(yù)壞死性小腸結(jié)腸炎新生大鼠組(NEC+BIF組)。3組于第1、2、3天固定時(shí)間點(diǎn)測(cè)量體質(zhì)量;第4天處死大鼠,HE染色觀察回盲部腸組織病理學(xué)變化并行病理學(xué)評(píng)分,異硫氰酸熒光素-葡聚糖(FITCDextran)示蹤法檢測(cè)腸黏膜通透性,ELISA檢測(cè)血清Zonulin的水平,Western blot及免疫組化檢測(cè)腸組織緊密連接蛋白的表達(dá)及分布,將3組大鼠血清Zonulin水平與腸道通透性及緊密連接蛋白表達(dá)量行相關(guān)性分析。結(jié)果與NEC組相比,雙歧桿菌干預(yù)后大鼠腸上皮損傷明顯改善;而與對(duì)照組相比,NEC組腸道通透性顯著增高(P0.05),血清Zonulin表達(dá)明顯增加(P0.05),腸上皮緊密連接蛋白Claudin-1、Occludin、ZO-1的表達(dá)明顯減少(P0.05),且分布不連續(xù);與NEC組相比,NEC+BIF組腸道通透性降低(P0.05),血清Zonulin表達(dá)降低(P0.05),腸上皮緊密連接蛋白的表達(dá)增加(P0.05)。相關(guān)性分析顯示,3組大鼠血清Zonulin水平與腸道通透性呈正相關(guān)(r=0.923,P0.01),而血清Zonulin水平與腸組織緊密連接Claudin-1、Occludin、ZO-1蛋白表達(dá)量均呈負(fù)相關(guān)(r=-0.779,P0.01;r=-0.920,P0.01;r=-0.619,P0.01)。結(jié)論雙歧桿菌可明顯改善NEC大鼠的腸道通透性,其機(jī)制可能與抑制Zonulin的表達(dá)有關(guān)。
[Abstract]:Objective to investigate the effects of Bifidobacterium on intestinal permeability and serum Zonulin expression in neonatal rats with necrotizing enterocolitis. Methods 60 Sprague-Dawley rats were randomly divided into 3 groups (n = 20): control group. The body mass of neonatal rats with necrotizing enterocolitis (NEC group) and group 3 (NEC BIF group) treated with Bifidobacterium were measured at a fixed time point of 2 days. On the 4th day, the rats were killed to observe the histopathological changes of ileocecal intestine and the pathological score by HE staining. Expression and distribution of tight junction protein in intestinal tissue were detected by FITCD extran tracer method. Serum level of Zonulin was detected by Elisa and the expression and distribution of tight junction protein in intestinal tissue were detected by immunohistochemistry. The correlation between serum Zonulin level and intestinal permeability and tight junction protein expression was analyzed. Results compared with NEC group, the intestinal epithelium injury of rats treated with Bifidobacterium was significantly improved. Compared with the control group, the intestinal permeability and serum Zonulin expression in NEC group were significantly increased (P0.05), and the expression of Claudin-1 Occludinia ZO-1 protein in intestinal epithelium was significantly decreased (P0.05), and the distribution was discontinuous. Compared with the NEC group, the intestinal permeability decreased, the serum Zonulin expression decreased (P0.05), and the intestinal epithelial tight junction protein expression increased (P0.05) in the NEC BIF group. The correlation analysis showed that the serum Zonulin level was positively correlated with intestinal permeability (r = 0.923, P0.01), while the serum Zonulin level was negatively correlated with the protein expression of Claudin-1 cludini protein in intestinal tissue (r-0.779P0.01-0.920P0.01r-0.619P0.01). Conclusion Bifidobacterium can obviously improve the intestinal permeability of NEC rats, and its mechanism may be related to the inhibition of Zonulin expression.
【作者單位】： 重慶醫(yī)科大學(xué)附屬兒童醫(yī)院新生兒科 兒童發(fā)育疾病研究省部共建教育部重點(diǎn)實(shí)驗(yàn)室 兒科學(xué)重慶市重點(diǎn)實(shí)驗(yàn)室 重慶市兒童發(fā)育重大疾病診治與預(yù)防國(guó)際科技合作基地;
【基金】：國(guó)家自然科學(xué)基金(30200300) 國(guó)家臨床重點(diǎn)專科建設(shè)項(xiàng)目經(jīng)費(fèi)基金(衛(wèi)辦醫(yī)政[2011]873)~~
【分類號(hào)】：R722.1;R-332

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