本文選題：薄型子宮內(nèi)膜 + 無水乙醇　； 參考：《中南大學(xué)》2011年碩士論文

【摘要】：目的：探索宮腔注射無水乙醇建立大鼠薄型子宮內(nèi)膜實驗?zāi)Ｐ偷姆椒?并鑒定模型是否成立。 方法：取性成熟未交配雌性具有動情周期的Sprague-Dawley(SD)大鼠25只,隨機分為兩組：對照組5只(10個子宮),實驗組20只(40個子宮)。對照組自宮角處注入生理鹽水,貯留5分鐘,實驗組自宮角處注入95%無水乙醇,分別貯留5分鐘、10分鐘。根據(jù)液體貯留時間不同,實驗組隨機分為5分鐘組、10分鐘組,各10只大鼠(20個子宮)。經(jīng)過兩個動情周期后,取處于動情期大鼠的子宮,測量子宮內(nèi)膜厚度：若實驗組子宮內(nèi)膜厚度與對照組相比無變化或者子宮內(nèi)膜層完全壞死,則造模失敗。若子宮內(nèi)膜變薄,我們將內(nèi)膜變薄的子宮歸為造模組,對其HE染色后子宮內(nèi)膜組織形態(tài)學(xué)進(jìn)行進(jìn)一步觀察,并采用免疫組織化學(xué)法方法檢測角蛋白、波形蛋白、VEGF、ERα的表達(dá),分析子宮內(nèi)膜形態(tài)學(xué)特點、子宮內(nèi)膜上皮細(xì)胞和間質(zhì)中細(xì)胞再生長情況以及VEGF、ERα表達(dá)情況是否符合薄型內(nèi)膜的特點,從而鑒定模型是否成功建立。 結(jié)果： 1.宮腔注射95%無水乙醇經(jīng)過兩個動情周期后,5分鐘組中,1只大鼠死亡,4個子宮的內(nèi)膜厚度恢復(fù)至正常,14個子宮的內(nèi)膜變��；10分鐘組中,2只大鼠死亡,8只大鼠子宮內(nèi)膜層壞死或者子宮全層壞死。 2.與對照組相比：造模組子宮壁全層明顯變薄,子宮內(nèi)膜層(764.1±100.2μmvs 234.6±48.1μm,p0.01)較肌層更明顯,且未見明顯波浪形；腺體稀疏,部分腺腔膨大成囊狀,腺上皮及腔上皮細(xì)胞呈低柱、立方甚至扁平狀；血管減少,部分管腔膨大。10分鐘組子宮內(nèi)膜層大部分為無細(xì)胞結(jié)構(gòu)區(qū)域,僅存少數(shù)細(xì)胞結(jié)構(gòu)輪廓；淺肌層甚至子宮全層壞死,肌層血管擴張,內(nèi)可見血栓形成。 3.造模組單位內(nèi)膜面積中角蛋白面積小于對照組(11.46±2.40%vs 17.82±2.28%,p0.05),差異有統(tǒng)計學(xué)意義；造模組單位間質(zhì)面積中波形蛋白面積小于對照組(8.30±2.84%vs 13.62±3.18%,p0.05),差異有統(tǒng)計學(xué)意義；造模組子宮內(nèi)膜上皮細(xì)胞和間質(zhì)細(xì)胞中VEGF平均光密度低于對照組(0.033±0.0053 vs 0.054±0.0084,p0.05),差異有統(tǒng)計學(xué)意義。造模組子宮內(nèi)膜上皮細(xì)胞中ERa組織學(xué)積分稍低于對照組(3.12±0.07 vs 3.20±0.12,p0.05),差異無統(tǒng)計學(xué)意義。 結(jié)論：初步證實95%無水乙醇0.5ml宮腔貯留5分鐘可以成功形成大鼠薄型子宮內(nèi)膜,造模成功率為70%。
[Abstract]:Aim: to explore the method of establishing rat model of thin endometrium by intrauterine injection of anhydrous alcohol and to identify whether the model was established. Methods: Twenty-five Sprague-Dawley (SD) rats with oestrous cycle were randomly divided into two groups: control group (n = 5) (n = 10) and experimental group (n = 20) (n = 40). The control group was injected with normal saline from the angle of uterus for 5 minutes, and the experimental group was injected with 95% anhydrous alcohol from the corner of the uterus for 5 minutes and 10 minutes respectively. According to the time of liquid storage, the experimental group was randomly divided into 5 minute group and 10 minutes group, each 10 rats (20 uteri). After two estrus cycles, the endometrium thickness of rats in estrus period was measured. If the endometrial thickness of the experimental group was not changed compared with the control group or the endometrium was completely necrotic, the model failed. If the endometrium became thinner, we classified the thinned endometrium as a model group, observed the morphology of endometrium after HE staining, and detected the expression of keratin, vimentin and VEGFER 偽 by immunohistochemical method. The morphological characteristics of endometrium, the growth of endometrial epithelial cells and stromal cells, and the expression of VEGF ER 偽 were analyzed. Results: 1. After two estrous cycles, one rat died, four endometrium thickness returned to normal, and 14 endometrium became thinner in uterine cavity injection of 95% anhydrous alcohol after two estrous cycles. In the 10-minute group, 2 rats died and 8 rats died of endometrial necrosis or full uterine necrosis. 2. Compared with the control group, the endometrium layer (764.1 鹵100.2 渭 mvs 234.6 鹵48.1 渭 m P0.01) was significantly thinner than that of the control group (764.1 鹵100.2 渭 mvs 234.6 鹵48.1 渭 m P0.01), and there was no obvious wavy shape in the endometrial layer, and the glands were sparse, some of the glandular lumens were cystic, the glandular epithelium and the lumen epithelial cells were low columnar. Cuboidal or even flattened; reduced blood vessels. The endometrial layer of partial lumen bulging for 10 minutes was mostly cell-free, with only a few cell contours; superficial muscle layer and even the whole uterine layer were necrotic, myometrium vasodilated. Intrinsic thrombus formation. 3. The median area of keratin per unit intima in the model group was smaller than that in the control group (11.46 鹵2.40%vs 17.82 鹵2.28), and the area of vimentin in the interstitial area of the model group was lower than that in the control group (8.30 鹵2.84%vs 13.62 鹵3.18p0.05). The average optical density in endometrial epithelial cells and stromal cells in model group was significantly lower than that in control group (0.033 鹵0.0053 vs 0.054 鹵0.0084 p0.05). The ERa histological score in endometrial epithelial cells in the model group was slightly lower than that in the control group (3.12 鹵0.07 vs 3.20 鹵0.12p0.05). Conclusion: 95% anhydrous ethanol 0.5ml uterine cavity storage for 5 minutes can successfully form rat thin endometrium, and the success rate of modeling is 70.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R-332

【參考文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 周曉曦;原因不明月經(jīng)過少子宮內(nèi)膜中血管內(nèi)皮生長因子及基質(zhì)金屬蛋白酶-11的研究[D];重慶醫(yī)科大學(xué);2007年

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