本文選題：兩次打擊 + 迷走神經(jīng)切斷��； 參考：《遵義醫(yī)學(xué)院》2011年碩士論文

【摘要】：目的：通過復(fù)制“二次打擊”SIRS大鼠模型,探討迷走神經(jīng)切斷及煙堿預(yù)處理對大鼠SIRS的作用,為臨床治療SIRS提供新思路。 方法：選擇雄性健康SD大鼠40只,隨機(jī)分為四組,每組10只。對照組在誘導(dǎo)SIRS模型前7d未予處理；單側(cè)迷走神經(jīng)切斷組：在誘導(dǎo)SIRS模型前7d,行右側(cè)頸部迷走神經(jīng)切斷；煙堿預(yù)處理組：在誘導(dǎo)SIRS模型前7d,開始每天給予煙堿液5mg. kg-1ip;聯(lián)合迷走神經(jīng)切斷+煙堿預(yù)處理組：在誘導(dǎo)SIRS模型前7d,聯(lián)合迷走神經(jīng)切斷+煙堿預(yù)處理。單側(cè)迷走神經(jīng)切斷術(shù)采用腹頸正中切口顯露,切斷右側(cè)頸部迷走神經(jīng)主干。造模時(shí)采用腹腔注射麻醉,制作“二次打擊”(休克及腹腔內(nèi)注入LPS)動(dòng)物模型。采集血液標(biāo)本或器官組織進(jìn)行相關(guān)檢測。檢測結(jié)果進(jìn)行統(tǒng)計(jì)學(xué)分析處理,比較四組大鼠TNF-a、IL-6含量；外周血白細(xì)胞計(jì)數(shù)與分類；血生化及肺、肝、腎的病理學(xué)變化。 結(jié)果：迷走神經(jīng)切斷后能提高炎癥反應(yīng)并加重大鼠重要臟器的損傷。煙堿預(yù)處理后能降低炎癥反應(yīng),并對重要臟器有保護(hù)作用。聯(lián)合處理后炎癥反應(yīng)及重要臟器損傷程度介于上述兩者之間,與對照組比較仍有統(tǒng)計(jì)學(xué)意義。 結(jié)論：中樞神經(jīng)能夠通過副交感神經(jīng)途徑調(diào)節(jié)炎癥反應(yīng)；煙堿能抗炎且對重要臟器有一定保護(hù)作用,其可不依賴于迷走神經(jīng)的完整性而獨(dú)立發(fā)揮作用。
[Abstract]:Objective: to study the effect of vagotomy and nicotine pretreatment on Sirs in rats by making "second strike" Sirs rat model, and to provide a new idea for clinical treatment of Sirs. Methods: forty male healthy SD rats were randomly divided into four groups with 10 rats in each group. In the control group, the Sirs model was not treated 7 days before induction, and in the unilateral vagotomy group, the right cervical vagotomy was performed 7 days before the Sirs model was induced. Nicotine pretreatment group: 7 days before Sirs model was induced, 5 mg 路kg ~ (-1) IP of nicotine solution was given daily, and 7 days before induction of Sirs model, combined vagotomy nicotine pretreatment group was combined with vagus nerve transection nicotine pretreatment group. Unilateral vagotomy was performed by median incision of abdominal neck and transection of right cervical vagus nerve trunk. The animal model of second strike (shock and intraperitoneal injection of LPSs) was made by intraperitoneal injection of anesthesia. Blood samples or organ tissues were collected for relevant examination. The results were statistically analyzed to compare the contents of TNF-a IL-6, the count and classification of peripheral blood leukocytes, the pathological changes of blood biochemistry, lung, liver and kidney in the four groups. Results: vagotomy can increase the inflammatory response and aggravate the injury of important organs in rats. Nicotine pretreatment can reduce inflammation and protect important organs. The degree of inflammatory reaction and injury of important organs after combined treatment was between the above two groups, which was still statistically significant compared with the control group. Conclusion: CNS can regulate inflammatory response through parasympathetic pathway, nicotine can resist inflammation and protect important organs, and it can play an independent role independent of the integrity of vagus nerve.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 石瑩;王曉勇;;血必凈對全身炎癥反應(yīng)綜合征的治療作用[J];浙江中醫(yī)藥大學(xué)學(xué)報(bào);2009年04期

2 解福平,郭建玲,賈鳳菜;新生兒全身性炎癥反應(yīng)綜合征的臨床分析[J];中國醫(yī)師雜志;2000年01期

3 黎君友;胡森;孫曉慶;嚴(yán)鳴;晉樺;姜小國;周寶桐;盛志勇;;創(chuàng)傷膿毒癥早期病理生理過程的探討[J];感染.炎癥.修復(fù);2000年02期

4 韋和平,劉達(dá)恩;嚴(yán)重?zé)齻c全身炎癥反應(yīng)綜合征[J];廣西醫(yī)學(xué);2000年06期

5 陳中件,季清銳,龐福珍,倪新山,龐黎華;新生兒窒息發(fā)生SIRS和MODS的臨床分析[J];河北醫(yī)學(xué);2001年03期

6 祝益民,肖政輝,胥志躍,趙祥文;全身性炎性介質(zhì)監(jiān)測在急性危重病患兒臨床研究中的作用[J];小兒急救醫(yī)學(xué);2000年04期

7 周國強(qiáng),汪良,陳彥;“兩次打擊”全身炎癥反應(yīng)綜合征大鼠模型的建立[J];蘇州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2003年03期

8 周迎宏;;大黃在危重病治療中的應(yīng)用進(jìn)展[J];現(xiàn)代醫(yī)學(xué);2009年01期

9 張銀中,葉鐵虎,趙麗云,任洪智,黃宇光;細(xì)胞因子白介素-6和白介素-10對中性粒細(xì)胞凋亡的影響[J];武警醫(yī)學(xué);2004年09期

10 雒云祥;周領(lǐng);李洪艷;李蓮芝;;大黃治療全身炎癥反應(yīng)綜合征的探討[J];右江醫(yī)學(xué);2006年06期

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