本文選題：綠色熒光蛋白 + 裸小鼠腦��； 參考：《蘇州大學(xué)》2012年碩士論文

【摘要】：目的:借助立體定位術(shù)和熒光來界定GFP裸小鼠的腦解剖結(jié)構(gòu)。 方法:對(duì)GFP裸小鼠腦分別進(jìn)行冠狀位、矢狀位和水平位的冰凍切片，經(jīng)熒光成像后行尼氏染色做對(duì)比觀察，結(jié)合Paxinos編著的小鼠腦圖譜對(duì)熒光圖像內(nèi)的解剖結(jié)構(gòu)進(jìn)行辨認(rèn)分析。通過立體定位儀定位10只成年GFP裸小鼠的前囟和人字點(diǎn)所在的腦冠狀切面，然后進(jìn)行冰凍切片，比較小鼠個(gè)體間以及與小鼠腦圖譜間的解剖結(jié)構(gòu)差異。利用小鼠腦切片模具分別對(duì)10個(gè)離體鼠腦進(jìn)行冠狀位和矢狀位上的切片，再進(jìn)行冰凍切片，，熒光鏡下觀察，比較個(gè)體間差異，并對(duì)離體鼠腦結(jié)構(gòu)進(jìn)行定位。 結(jié)果:GFP鼠腦的不同結(jié)構(gòu)間存在著熒光色差，借此可辨認(rèn)出鼠腦的解剖結(jié)構(gòu)：細(xì)胞核、尼氏小體密集區(qū)及神經(jīng)纖維分布區(qū)為弱熒光信號(hào)，細(xì)胞核、尼氏小體密集區(qū)周圍結(jié)構(gòu)，如嗅球的叢狀層、小腦的分子層為強(qiáng)熒光信號(hào)。切片經(jīng)尼氏染色后，熒光強(qiáng)度明顯減弱，體視顯微鏡下觀察到的腦結(jié)構(gòu)與熒光鏡觀察的結(jié)果基本一致，參照小鼠腦圖譜可對(duì)熒光圖像上的解剖結(jié)構(gòu)進(jìn)行辨認(rèn)。對(duì)在體鼠腦的立體定位研究發(fā)現(xiàn)GFP小鼠間的前囟、人字點(diǎn)處的冠狀切面結(jié)構(gòu)無差異，但前囟處的冠狀切面結(jié)構(gòu)與圖譜中前囟處的冠狀切面結(jié)構(gòu)不同。對(duì)離體鼠腦的立體定位研究并未發(fā)現(xiàn)個(gè)體間的腦切片有差異性，利用小鼠切片模具可進(jìn)行離體鼠腦的解剖結(jié)構(gòu)定位，準(zhǔn)確快捷地獲得興趣位點(diǎn)。 結(jié)論:借助立體定位術(shù)和熒光界定GFP裸小鼠的腦解剖結(jié)構(gòu)可做為一個(gè)實(shí)驗(yàn)平臺(tái)應(yīng)用于目前廣為開展的熒光示蹤實(shí)驗(yàn)的解剖定位研究。
[Abstract]:Aim: to determine the brain anatomy of GFP nude mice by stereotactic localization and fluorescence. Methods: frozen sections in coronal, sagittal and horizontal position of the brain of GFP nude mice were made respectively. The anatomical structure of the fluorescence image was identified and analyzed with the help of the mouse brain atlas compiled by Paxinos. The frontal fontanelle of 10 adult GFP nude mice and the coronal section of the human point were located by stereotactic locator, and then frozen sections were made to compare the anatomical structure difference between the mice and the mouse brain atlas. The coronal and sagittal sections of 10 isolated brains were made by using the mouse brain slice mould, and then frozen sections were observed under fluorescence microscope to compare the differences between individuals, and to locate the brain structure in vitro. Results there were fluorescence color aberrations among the different structures of the brain. The anatomical structure of the brain could be identified: the nucleus, the dense area of Nissl body and the distribution of nerve fiber were weak fluorescence signals, the nucleus, the structure around the dense area of Nissl corpuscles. The molecular layer of the cerebellum, such as the plexiform layer of the olfactory bulb, is highly fluorescent. The fluorescence intensity of the slices was obviously weakened after stained by Nissl, and the observed brain structure under stereoscopic microscope was basically consistent with that observed by fluorescence microscope. The anatomical structure of the fluorescence image could be identified by referring to the mouse brain map. The stereotactic study of the brain in vivo showed that there was no difference in the structure of the coronal section between the GFP mice and the herringbone, but the coronal section structure at the anterior fontanel was different from that at the middle fontanel of the map. The stereotactic localization of the isolated brain has not found any difference among individuals. The mouse slice mould can be used to locate the anatomical structure of the brain in vitro and to obtain the site of interest accurately and quickly. Conclusion: stereotactic localization and fluorescence determination of the brain anatomy of GFP nude mice can be used as an experimental platform to study the anatomical localization of fluorescent tracer experiments.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R322

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相關(guān)期刊論文 前2條

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本文編號(hào)：2018991