本文選題：漢坦病毒 + 細(xì)胞培養(yǎng)　； 參考：《中國媒介生物學(xué)及控制雜志》2016年05期

【摘要】：目的對2012年河北省鼠類標(biāo)本進(jìn)行漢坦病毒(HV)分離并復(fù)蘇保存的病毒株,鑒定型別,了解河北省疫區(qū)HV的型別分布特點(diǎn)。方法利用間接免疫熒光法(IFA)對鼠肺標(biāo)本進(jìn)行初篩,采用細(xì)胞培養(yǎng)法分離陽性標(biāo)本病毒,并復(fù)蘇已保存的病毒株。提取病毒RNA后進(jìn)行分型鑒定。結(jié)果 4份陽性鼠肺標(biāo)本的組織懸液接種Vero-E6細(xì)胞2~3代后,經(jīng)IFA檢測,細(xì)胞內(nèi)出現(xiàn)病毒特異性熒光顆粒,但未發(fā)現(xiàn)致細(xì)胞病變效應(yīng)。4份標(biāo)本全部分離成功。取已保存的病毒株接種Vero-E6細(xì)胞1~2代后,經(jīng)IFA檢測,細(xì)胞內(nèi)出現(xiàn)較強(qiáng)的病毒特異性熒光顆粒,6株病毒全部復(fù)蘇成功。通過分型鑒定,10株病毒均屬于家鼠型漢城型漢坦病毒(SEOV)。結(jié)論河北省HV以SEO型為主。SEOV在Vero-E6細(xì)胞雖無明顯的細(xì)胞病變,但可在該細(xì)胞中增殖并被檢測。在-80℃條件下,HV至少保存20余年。我國東方田鼠為SEOV的宿主之一。
[Abstract]:Objective to isolate and resuscitate the strain of Hantavirus (HVV) from rat samples in Hebei Province in 2012, to identify the type of HV and to understand the distribution characteristics of HV in epidemic area of Hebei Province. Methods indirect immunofluorescence assay (IFA) was used to screen the lung samples of mice. The positive specimens were isolated by cell culture and the preserved virus strains were resuscitated. The virus RNA was extracted and identified. Results Vero-E6 cells were inoculated with the tissue suspensions of 4 positive mice lung specimens for 2 ~ 3 passages. After IFA detection, virus specific fluorescent particles were found in the cells, but none of the 4 specimens were isolated successfully. After the preserved virus strain was inoculated into Vero-E6 cells for 1 and 2 passages, the results of IFA showed that all of the 6 virus strains with strong virus specific fluorescence particles were successfully resuscitated. It was identified by typing that all 10 strains belonged to the Seoul type of Hantavirus of domestic mouse type SEOVV. Conclusion HV of Hebei Province is mainly SEO type. SEOV has no obvious cytopathic effect in Vero-E6 cells, but it can proliferate and be detected in Vero-E6 cells. HV was preserved at -80 鈩，

本文編號：2008773