發(fā)布時間：2018-06-12 00:11

  本文選題：溫陽振衰顆粒 + 慢性心衰��； 參考：《中國藥房》2017年34期

【摘要】：目的:研究溫陽振衰顆粒對慢性心衰(CHF)模型大鼠心肌中肌細(xì)胞增強(qiáng)子(MEF2)表達(dá)的影響。方法:將60只大鼠隨機(jī)抽取10只作為正常對照組(生理鹽水),其余50只大鼠以阿霉素建立CHF模型后隨機(jī)分為模型對照組(生理鹽水)、陽性對照組[依那普利1.8 mg/(kg·d)]和溫陽振衰顆粒低、中、高劑量組[0.72、1.44、2.88 g/(kg·d)],每天ig給藥2次,連續(xù)給藥4周。給藥結(jié)束后,檢測各組大鼠心功能指標(biāo)[左心室射血分?jǐn)?shù)(LVEF)、左心室短軸縮短率(LVFS)、左心室收縮末期內(nèi)徑(LVSD)和左心室舒張末期內(nèi)徑(LVDD)],檢測各組大鼠心肌中MEF2和磷酸化MEF2(p-MEF2)mRNA及其蛋白的表達(dá)。結(jié)果:與正常對照組比較,模型對照組和各給藥組大鼠LVEF、LVFS明顯降低(P0.05),LVSD、LVDD明顯升高(P0.05);心肌中p-MEF2蛋白表達(dá)明顯下調(diào)(P0.05),MEF2 mRNA及蛋白表達(dá)差異無統(tǒng)計(jì)學(xué)意義(P0.05)。與模型對照組比較,各給藥組大鼠心功能指標(biāo)均明顯改善(P0.05);心肌中p-MEF2蛋白表達(dá)均明顯上調(diào)(P0.05),MEF2 mRNA及蛋白表達(dá)差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:溫陽振衰顆粒能拮抗阿霉素誘導(dǎo)的MEF2蛋白磷酸化的下調(diào)作用,這可能是其治療CHF的機(jī)制之一。
[Abstract]:Aim: to study the effect of Wenyang Zhenshuai granule (WJG) on the expression of myocyte enhancer MEF2 in rat model of chronic heart failure (CHF). Methods: ten rats were randomly selected as normal control group (normal saline group), and the other 50 rats were randomly divided into model control group (normal saline group), positive control group [enalapril 1.8 mg/(kg d] after doxorubicin was used to establish CHF model. And Wenyang Zhenshuai granules are low, The middle and high dose group [0.72V 1.44U 2.88 g/(kg d] was given intravenously twice a day for 4 weeks. After the administration, Left ventricular ejection fraction (LVVEFN), left ventricular shortening rate (LVFSN), left ventricular end-systolic diameter (LVSD) and left ventricular end-diastolic diameter (LVDDD) were measured, and the expression of MEF2 and phosphorylated MEF2Op-MEF2 mRNA and protein were detected. Results: compared with the normal control group, LVEFV LVFS in the model control group and each administration group was significantly lower than that in the control group, and the LVDD of LVSD in the LVSD group was significantly higher than that in the normal control group, while the expression of p-MEF2 protein in the myocardium was significantly lower than that in the control group, and there was no significant difference in the expression of P0.05 and MEF2 mRNA and protein between the two groups. Compared with the model control group, the cardiac function indexes of rats in each administration group were significantly improved, and the expression of p-MEF2 protein was significantly up-regulated in myocardium. There was no significant difference in mRNA and protein expression between the two groups. Conclusion: Wenyang Zhenshuai granule can antagonize the down-regulation of MEF2 protein phosphorylation induced by adriamycin, which may be one of the mechanisms of its treatment of CHF.
【作者單位】： 湖南中醫(yī)藥大學(xué)第一附屬醫(yī)院科研科;貴陽中醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)院;湖南中醫(yī)藥大學(xué)第一附屬醫(yī)院中心重癥加強(qiáng)護(hù)理病房;湖南中醫(yī)藥大學(xué)第一附屬醫(yī)院;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(No.81173213、81704-061) 湖南省教育廳資助項(xiàng)目(No.15C1047) 2016年度湖南省中醫(yī)藥科研計(jì)劃項(xiàng)目(No.201651)
【分類號】：R285.5;R-332

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本文編號：2007310