本文選題：霧霾PM. + 慢性阻塞性肺疾病�。� 參考：《中華中醫(yī)藥雜志》2017年11期

【摘要】：目的:對霧霾PM2.5建立的慢性阻塞性肺疾病(COPD)大鼠模型可靠性及清肺化濁行血法治療霧霾形成的COPD療效作出評價。方法:90只雄性Wistar大鼠隨機分為兩組:正常對照組(30只)、COPD模型組(60只),采用暗火造成霧霾環(huán)境煙熏加氣管滴注脂多糖的造模方法建立COPD模型,并將其分為B1、B2、B3、B4組。B1組留取標本,飼養(yǎng)30d后處死;B2、B3、B4組大鼠撤去一切造模因素后正常飼養(yǎng)。B3、B4組分別給予相應(yīng)藥物干預(yù)治療,連續(xù)4周,后觀察大鼠一般情況、肺功能及血清中白介素-8(IL-8)和白介素-10(IL-10)含量情況,并對其病理標本進行檢測。結(jié)果:COPD大鼠模型的一般情況與其臨床表現(xiàn)相符,大鼠體質(zhì)量減輕,吸氣阻力和呼氣阻力升高、肺順應(yīng)性下降,但血清IL-8、IL-10濃度與正常組相比,差異顯著(P0.05),病理切片顯示肺組織出現(xiàn)炎性損傷。用康肺散結(jié)化濁行血湯和布地奈德干預(yù)的模型大鼠癥狀好轉(zhuǎn),肺功能改善,血清IL-10濃度與模型組大鼠相比,顯著降低(P0.05),病理切片示炎性損傷明顯減輕。結(jié)論:用本實驗方法建立COPD大鼠模型是可行的,康肺散結(jié)化濁行血湯在一定程度上能夠改善COPD大鼠模型的癥狀。
[Abstract]:Objective: to evaluate the reliability of rat model of chronic obstructive pulmonary disease (COPDD) induced by PM2.5 haze and the efficacy of clearing lung and removing turbid blood in treating COPD caused by smog. Methods 90 male Wistar rats were randomly divided into two groups: normal control group (n = 30) and COPD model group (n = 60). The COPD model was established by smoke fumigation and endotracheal instillation of lipopolysaccharide (LPS) in the smog environment caused by dark fire, and the model was divided into two groups: group B _ (1) B _ (2) B _ (2) B _ (3) B _ (4) and group B _ (1). After 30 days of feeding, the rats in group B _ 2 B _ 3 B _ 3H _ 4 were killed and all the model factors were removed. The rats in group B _ (3) B _ (4) were given corresponding drug intervention for 4 weeks respectively. The general condition, lung function and the contents of interleukin-8IL-8 (IL-8) and interleukin-10 (IL-10) in serum of rats were observed. The pathological specimens were examined. Results the general situation of the model was consistent with its clinical manifestation. The weight of rats was reduced, inspiratory resistance and expiratory resistance were increased, and lung compliance was decreased, but the concentration of IL-8 IL-10 in serum was higher than that in normal group. The difference was significant (P 0.05). Pathological sections showed inflammatory injury in lung tissue. The model rats treated with Kangfei Sanjie Huazhuo decoction and budesonide improved their symptoms and pulmonary function. Compared with the model group, the concentration of IL-10 in serum decreased significantly (P 0.05), and the pathological section showed that the inflammatory injury was alleviated obviously. Conclusion: it is feasible to establish COPD rat model by this method. Kangfei Sanjie Huazhuazhuoxue decoction can improve the symptoms of COPD rat model to some extent.
【作者單位】： 煙臺市中醫(yī)醫(yī)院治未病中心;山東中醫(yī)藥大學(xué);河南中醫(yī)藥大學(xué)第三附屬醫(yī)院;
【基金】：山東省重點研發(fā)計劃項目(No.2015GSF119036)~~
【分類號】：R285.5;R-332

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