本文選題：HN + 流感疫苗　； 參考：《中國生物制品學(xué)雜志》2016年05期

【摘要】：目的評(píng)價(jià)人甲型H7N9禽流感全病毒滅活疫苗在小鼠中的免疫原性。方法分別將H7N9與H1N1、H5N1全病毒滅活疫苗稀釋至血凝素抗原(hemagglutinin,HA)濃度分別為30、6、1.2、0.24和0.048μg/ml后,免疫BALB/c小鼠,各10只,0.5 ml/只,采用血凝抑制(hemagglutinin inhibition,HI)試驗(yàn)檢測(cè)免疫后小鼠血清抗體滴度,計(jì)算抗體幾何平均滴度(GMT)及半數(shù)有效劑量(ED_(50)),并檢測(cè)免疫后小鼠血清對(duì)其他亞型病毒的HI滴度。結(jié)果 H7N9組小鼠陽轉(zhuǎn)率與H1N1組較相近,僅H7N9 0.6μg劑量組GMT明顯低于H1N1同劑量組(P0.001),其余劑量組差異無統(tǒng)計(jì)學(xué)意義(P0.05);H7N9與H1N1、H5N1全病毒滅活疫苗免疫小鼠ED_(50)分別為0.27、0.12和15μg HA。免疫后血清對(duì)其他亞型病毒無交叉反應(yīng)。結(jié)論 H7N9疫苗在小鼠中的免疫原性略低于H1N1疫苗,但明顯優(yōu)于H5N1疫苗。
[Abstract]:Objective to evaluate the immunogenicity of inactivated human avian influenza A (A H 7 N 9) vaccine in mice. Methods the hemagglutininine (HA) antigen-hemagglutininine (HA) concentration of H7N9 and H _ 1N _ 1 inactivated H5N1 vaccine were diluted to 30 ~ 61.2g ~ 0.24 渭 g/ml and 0.048 渭 g/ml, respectively. BALB / c mice were immunized with hemagglutinin inhibition (HI) test, and the antibody titer was detected by hemagglutinin inhibition test. The geometric mean titer of antibodies (GMTT) and the half effective dose of EDTs were calculated and the HI titers of serum against other subtypes of viruses were detected. Results the positive conversion rate of H7N9 group was similar to that of H1N1 group, but the GMT of H7N9 0.6 渭 g group was significantly lower than that of H1N1 same dose group (P0.001). There was no significant difference in other dose groups (0.270.12 渭 g vs 15 渭 g HA). After immunization, the serum did not cross react to other subtypes of virus. Conclusion the immunogenicity of H _ 7N _ 9 vaccine in mice was slightly lower than that of H1N1 vaccine, but significantly superior to that of H5N1 vaccine.
【作者單位】： 中國食品藥品檢定研究院衛(wèi)生部生物技術(shù)產(chǎn)品檢定方法及其標(biāo)準(zhǔn)化重點(diǎn)實(shí)驗(yàn)室;北京科興生物制品有限公司;
【基金】：國家國際科技合作與交流專項(xiàng)(2013DFA31680)
【分類號(hào)】：R373.13;R392

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 姚昕;毛群穎;梁爭論;;EV71全病毒滅活疫苗的研究進(jìn)展[J];中國生物制品學(xué)雜志;2012年10期

相關(guān)會(huì)議論文 前1條

1 陳列勝;馬相虎;蔡明勇;周峰;楊菲茹;陳哲文;吳金妍;;流行性感冒病毒裂解疫苗的研制[A];預(yù)防醫(yī)學(xué)學(xué)科發(fā)展藍(lán)皮書（2006卷）[C];2006年

相關(guān)重要報(bào)紙文章 前1條

1 岳奎元;病毒,真的就十惡不赦？[N];四川科技報(bào);2012年

，

本文編號(hào)：2000985