本文選題：肺纖維化 + IL-��； 參考：《中國免疫學(xué)雜志》2017年10期

【摘要】：目的:初步研究博來霉素致肺纖維化大鼠肺組織中IL-37、IL-18的表達(dá)水平,探討其在PF發(fā)生、發(fā)展中的表達(dá)及意義。方法:將45只清潔級Wistar大鼠按隨機(jī)數(shù)字表法分為健康對照組(N組)、博來霉素組(B組)、地塞米松治療組(D組),每組大鼠各15只,B組及D組大鼠給予氣管內(nèi)注射博來霉素4 mg/kg制作肺纖維化模型,N組給予相同體積生理鹽水作為對照,D組大鼠于肺纖維化造�；A(chǔ)上第2天每日腹腔注射地塞米3 mg/kg,N、B組大鼠腹腔注射生理鹽水作為對照。各組大鼠分別于造模后第7、14、28天處死,HE染色評價(jià)肺組織病理形態(tài)學(xué)變化,堿水解法及酶聯(lián)免疫吸附法分別測定肺組織勻漿中羥脯氨酸(HYP)、IL-37含量,RT-PCR法測定肺組織中IL-18mRNA表達(dá)量。結(jié)果:(1)HE染色顯示B、D組大鼠肺組織病理學(xué)改變由肺泡炎癥逐漸發(fā)展為纖維化,B、D組HYP含量隨時(shí)間推移逐漸升高,以第28天為著(P0.05);(2)B、D組IL-37、IL-18mRNA表達(dá)于第7天升高至最高點(diǎn),后逐漸降低,至第28天均高于N組,差異有統(tǒng)計(jì)學(xué)意義(P0.05);(3)D組IL-37、IL-18mRNA表達(dá)在同一時(shí)間均低于B組(P0.05)。結(jié)論:IL-37、IL-18在大鼠肺纖維化發(fā)病早期起重要作用,可能參與了肺纖維化發(fā)生與發(fā)展。
[Abstract]:Objective: To investigate the expression level of IL-37 and IL-18 in lung tissue of rats with bleomycin induced pulmonary fibrosis, and to explore its expression and significance in the occurrence and development of PF. Methods: 45 clean grade Wistar rats were divided into healthy control group (N group), bleomycin group (B group), dexamethasone group (group D), 15 rats in each group, B Group and D rats were given intratracheal injection of bleomycin 4 mg/kg to make pulmonary fibrosis model. Group N was given the same volume of physiological saline as control. Group D rats were injected with 3 mg/kg, N, and B group of rats in group D daily on the basis of pulmonary fibrosis model. The rats in group B were injected with physiological saline as control. The rats in each group were given 7,14,28 after the model respectively. The pathological changes of lung tissue were evaluated by HE staining. Hydroxyproline (HYP) in lung tissue was measured by alkaline hydrolysis and enzyme linked immunosorbent assay (ELISA). The content of IL-37 and the expression of IL-18mRNA in lung tissue were measured by RT-PCR. Results: (1) HE staining showed B, and the pathological changes of lung tissue in group D were gradually developed from alveolar inflammation to fibrosis, B, The content of HYP in group D gradually increased with time, in twenty-eighth days (P0.05); (2) B, D group IL-37, IL-18mRNA expression increased to the highest point in seventh days, and then gradually decreased to twenty-eighth days, and the difference was statistically significant (P0.05). (3) D group IL-37, IL-18mRNA expression was lower than that in the same time. Early onset of chemotherapy may play an important role in the occurrence and development of pulmonary fibrosis.
【作者單位】： 佳木斯大學(xué)附屬第一醫(yī)院;
【基金】：佳木斯大學(xué)校級面上項(xiàng)目(JMSUJCMS2016-049)和佳木斯大學(xué)研究生科技創(chuàng)新項(xiàng)目醫(yī)藥類重點(diǎn)項(xiàng)目(YZ2016-019)
【分類號(hào)】：R-332;R563

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本文編號(hào)：1994138