本文選題：癌侵襲鏡像痛 + CQ復方��； 參考：《中國中藥雜志》2017年04期

【摘要】：該研究旨在了解CQ復方對癌侵襲鏡像痛(cancer invasion induced mirror image pain,CIIMIP)模型小鼠的鎮(zhèn)痛作用及其相關中樞機制。將雄性BALB/c小鼠隨機分為正常組、操作對照組(注射0.2 m L滅活的S180肉瘤細胞液)、模型組(于右腿股骨大轉子處注射0.2 m L S180肉瘤細胞液)、CQ復方低劑量組(模型+100 mg·kg~(-1),ip)、CQ復方中劑量組(模型+150 mg·kg~(-1),ip)、CQ復方高劑量組(模型+200 mg·kg~(-1),ip),造模前及術后用Von Frey纖維絲測定鏡像側后足的機械縮足閾值(mechanical withdrawal threshold,MWT);采用高效液相-熒光法(HPLC-FLD)檢測脊髓L3~L5節(jié)段內(nèi)谷氨酸(Glu)、γ-氨基丁酸(GABA)、甘氨酸(Gly)、�；撬�(Tau)濃度;采用Aim Plex流式高通量多因子檢測技術檢測L3~L5節(jié)段脊髓組織內(nèi)調節(jié)激活T細胞表達與分泌因子(RANTES)、單核細胞趨化蛋白(MCP-3)的含量;并觀察GABAa受體拮抗劑(荷包牡丹堿)對CQ復方鎮(zhèn)痛作用的影響。研究結果發(fā)現(xiàn),CQ復方能夠顯著提高模型小鼠的MWT(P0.01,P0.05),降低L3~L5節(jié)段脊髓組織內(nèi)興奮性氨基酸Glu的含量(P0.01,P0.05),提高抑制性氨基酸GABA,Gly,Tau的含量(P0.01,P0.05),降低Glu/GABA比值(P0.01,P0.05),降低RANTES,MCP-3水平(P0.05)。GABAa受體拮抗劑在2個時間點有意義地降低了CQ復方引起的MWT升高(P0.05)。該研究結果表明,CQ復方對CIIMIP模型小鼠有顯著的鎮(zhèn)痛作用,其機制與調節(jié)中樞神經(jīng)系統(tǒng)興奮性氨基酸(EAA)/抑制性氨基酸(IAA)遞質的平衡,部分激活GABAa受體,以及減少脊髓組織內(nèi)促炎性細胞因子RANTES,MCP-3的釋放有關。
[Abstract]:The purpose of this study was to investigate the analgesic effect of CQ compound on invasion induced mirror image CIIMIP model mice and its related central mechanism. The male BALB/c mice were randomly divided into normal group, Operation control group (0.2 mL inactivated S180 sarcoma cell fluid), model group (0.2 mL S180 sarcoma cell fluid injection at right leg trochanter), CQ compound low dose group (model 100 mg 路kg ~ (-1) CQ compound medium dose group (model 150 mg 路kg ~ (-1) In the high dose group (model 200mg / kg), Von Frey filament was used to measure the mechanical foot contraction threshold and mechanical withdrawal thresholdMWTA before and after the model, and the high performance liquid phase fluorescence (HPLC-FLDD) method was used to detect glutamate, 緯 -aminobutyrate GABAN, GABAN in the L3~L5 segment of spinal cord. The concentration of glycine, taurine Tau); The expression and secretion of regulatory activated T cells (RANTES), a monocyte chemoattractant protein (MCP-3), in L3~L5 segments of spinal cord were detected by Aim Plex flow high throughput multifactor assay. The effect of GABAa receptor antagonist (bicuculline) on CQ compound analgesia was observed. The results showed that CQ compound could significantly increase the level of MWTP 0.01 and P0.05, decrease the content of excitatory amino acid Glu in the spinal cord of L3~L5 segment and increase the content of inhibitory amino acid GABA Glycine Tau, decrease the ratio of Glu/GABA to Glu/GABA and decrease the level of RANTESm MCP-3 and the level of GABA A receptor. At two time points, the anti-agent significantly reduced the increase of MWT induced by CQ compound (P0. 05%). The results showed that CQ compound had a significant analgesic effect on CIIMIP model mice, and its mechanism was related to regulating the balance of excitatory amino acid (EAA) / inhibitory amino acid (EAA) transmitters in central nervous system (CNS) and partially activating GABAa receptors. And to reduce the release of the inflammatory cytokine RANTESN MCP-3 in spinal cord tissue.
【作者單位】： 中國中醫(yī)科學院醫(yī)學實驗中心北京市中醫(yī)藥防治重大疾病基礎研究重點實驗室;
【基金】：國家科技部國際科技合作專項(2010DFA31890) 中國中醫(yī)科學院自主選題研究項目(ZZ2013003)
【分類號】：R285.5;R-332

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