本文選題：阿薩希毛孢子菌 + 巨噬細胞γ-干擾素��； 參考：《安徽醫(yī)科大學》2012年碩士論文

【摘要】：研究目的 1.研究γ-干擾素對小鼠巨噬細胞株RAW264.7細胞吞噬、殺傷阿薩希毛孢子菌的作用。 2. γ-干擾素對小鼠巨噬細胞株RAW264.7體外抗阿薩希毛孢子菌殺傷機制的影響。 研究方法 1.不同濃度的γ-干擾素(IFN-γ)與RAW264.7細胞共孵育18h后，分別與阿薩希毛孢子菌（T.asahii）臨床株705共培養(yǎng)45min、4h。45min后在倒置顯微鏡下觀察瑞氏-姬姆薩染色RAW264.7細胞吞噬T.asahii的情況。4h后檢測RAW264.7細胞的殺傷活性。 2.不同濃度（10U/ml、100U/ml、1000U/ml）的IFN-γ與RAW264.7共同培養(yǎng)，再加入T.asahii培養(yǎng)后檢測菌落形成單位(CFU/ml），計算各濃度組的生長抑制率。在上述體系的上清液中分別檢測亞硝酸根離子（NO2-）。將巨噬細胞（Macrophage，Mφ）與不同終濃度的IFN-γ共同孵育后分別檢測超氧陰離子(O2-)的OD值。 結(jié)果 1. IFN-γ呈劑量依賴性地增強RAW264.7細胞吞噬、殺傷T. asahii的活性，各組之間的差異具有顯著性意義（P0.01）。 2. IFN-γ濃度分別為10U/ml、100U/ml、1000U/ml時，T.asahii的生長抑制率分別為25.21%、41.95%、55.83%，組間差異具有顯著統(tǒng)計學意義（P0.01）。上清液中NO2-的濃度分別為89.16±3.32、108.07±3.81、126.44±3.52，與空白對照組（74.47±1.48）相比差異具有顯著性意義(P0.01)。上清液中O2-的OD值分別為0.317±0.006、0.424±0.006、0.518±0.006，，與對照組相比差異具有顯著性意義（P0.01）。 結(jié)論 1.IFN-γ可增強RAW264.7細胞對T.asahii的吞噬、殺傷作用。 2.隨著IFN-γ濃度的增加，T.asahii生長抑制率增加。IFN-γ通過產(chǎn)生一氧化氮（NO）及O2-增強巨噬細胞體外抗T.asahii的殺傷活性。
[Abstract]:Research purpose 1. To study the effect of interferon 緯 on phagocytosis of murine macrophage cell line RAW264.7 and the killing of Asaxia sporophyllum. 2. The effect of interferon 緯 on the killing mechanism of murine macrophage cell line RAW264.7. Research method 1. Different concentrations of interferon 緯 (IFN- 緯) were co-incubated with RAW264.7 cells for 18 h. Cultured with T. asahii clinical strain 705 for 45 min, respectively, the killing activity of RAW264.7 cells was observed under inverted microscope. The T.asahii phagocytosis of RAW264.7 cells was detected by Rish-Giemsa staining for 4 h after incubating with T. asahii clinical strain 705 for 45 min. 2. IFN- 緯 was co-cultured with RAW264.7 at different concentrations of 10 U / ml ~ 100 U / ml ~ (-1) U ~ (-1 / ml). Then the colony forming unit (CFU / ml) was detected after T.asahii culture, and the growth inhibition rate of each concentration group was calculated. In the supernatant of the above system, the nitrite ion is determined. Macrophages (M 蠁) were incubated with IFN- 緯 with different final concentrations. The OD values of superoxide anion O _ 2-were determined. Result 1. IFN- 緯 enhanced the phagocytosis of RAW264.7 cells in a dose-dependent manner, killing the activity of T. asahii, and the difference was significant (P 0.01). 2. When the concentration of IFN- 緯 was 10 U / ml, 100 U / ml and 1000 U / ml, respectively, the growth inhibition rate of T. asahii was 25.21% and 41.95%, 55.83%, respectively. The difference between the two groups was statistically significant (P 0.01). The concentration of NO2- in supernatant was 89.16 鹵3.32108.07 鹵3.81126.44 鹵3.52, which was significantly different from that of control group (74.47 鹵1.48). The OD values of O _ 2- in supernatant were 0.317 鹵0.006, 0.424 鹵0.006, 0.518 鹵0.006, respectively, which were significantly different from those in the control group (P 0.01). Conclusion 1. IFN- 緯 could enhance the phagocytosis and cytotoxicity of RAW264.7 cells to T.asahii. 2. With the increase of IFN- 緯 concentration, the growth inhibition rate of T. asahii increased. IFN- 緯 increased the killing activity of macrophages against T.asahii by producing nitric oxide (no) and O 2 -.
【學位授予單位】：安徽醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R392

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