發(fā)布時(shí)間：2018-06-03 04:54

  本文選題：阪崎腸桿菌 + 生化特性�。� 參考：《新疆醫(yī)科大學(xué)》2011年碩士論文

【摘要】：目的：對(duì)阪崎腸桿菌新疆分離株的生物學(xué)特性進(jìn)行分析,通過乳鼠實(shí)驗(yàn)分析其器官侵襲特征,探討可能的毒力因素。方法：用結(jié)晶紫中性紅膽鹽葡萄糖瓊脂(VRBGA)酪胨大豆胨瓊脂(TSA)、腦心浸液培養(yǎng)液(BHI)、阪崎腸桿菌顯色培養(yǎng)基(DFI),酪蛋白氨基酸酵母浸膏培養(yǎng)液(CAYE)培養(yǎng)各菌株,觀察不同菌株的生長(zhǎng)特征；采用紙片擴(kuò)散法進(jìn)行抗生素的藥敏試驗(yàn)；阪崎腸桿菌新疆分離株經(jīng)產(chǎn)毒培養(yǎng)后對(duì)3～4日齡ICR乳鼠連續(xù)灌飼4日,并于第7日后無(wú)菌解剖取腸、肝、脾、腎等組織,石蠟切片,HE染色,光鏡觀察。結(jié)果：在不同培養(yǎng)基中不同菌株的生長(zhǎng)特征不完全相同,經(jīng)CAYE產(chǎn)毒培養(yǎng)液后,菌株表現(xiàn)出兩種不同的培養(yǎng)特征,有的產(chǎn)生絮狀沉淀伴有貼壁現(xiàn)象,有的則無(wú)絮狀沉淀且無(wú)貼壁現(xiàn)象,在其他培養(yǎng)基中的培養(yǎng)特征無(wú)差別；藥敏試驗(yàn)表明所有阪崎腸桿菌新疆分離株都對(duì)碳青霉烯類抗生素亞胺培南和美洛培南100%耐藥,對(duì)至少20種抗生素敏感；阪崎腸桿菌新疆分離株經(jīng)乳鼠實(shí)驗(yàn)測(cè)得腸尸比均小于0.083,顯示腸毒素陰性,但經(jīng)乳鼠灌飼實(shí)驗(yàn)觀察及病理切片分析,發(fā)現(xiàn)阪崎腸桿菌新疆分離株仍能造成乳鼠生長(zhǎng)發(fā)育遲緩和器官的損傷,并且這種損傷是全身性的,包括腸、肝、脾、腎等重要器官。結(jié)論：阪崎腸桿菌是腸桿菌科耐藥性不顯著的細(xì)菌,阪崎腸桿菌新疆分離株對(duì)機(jī)體具有潛在危害。
[Abstract]:Objective: to analyze the biological characteristics of Enterobacter sakazakii isolated from Xinjiang, and to explore the possible virulence factors by analyzing the organ invasion characteristics of the isolated strains of Enterobacter sakazakii. Methods: the strains were cultured with crystal violet neutral red bile salt glucose Agar (VRBGA) peptone soybean peptone Agar, brain heart extract (BHI), Enterobacter sakazakii color medium (DFI), casein amino acid yeast extract (CAYE), and the growth characteristics of different strains were observed. Enterobacter sakazakii strains isolated from Xinjiang were inoculated with enterobacter sakazakii for 4 days after inoculation for 4 days, and the tissues of intestine, liver, spleen and kidney were dissected on the 7th day. Paraffin sections were stained with HE and observed under light microscope. Results: the growth characteristics of different strains were not identical in different culture medium. After using CAYE medium, the strains showed two different culture characteristics, some of which produced flocculent precipitates and adhered to the wall. Some of them had no flocculent sedimentation and no adherence to the wall, and there was no difference in the culture characteristics in other media, and the drug sensitivity test showed that all isolates of Enterobacter sakazakii in Xinjiang were resistant to imipenem and melopenem antibiotics, which were 100% resistant to carbapenem antibiotics. Enterobacter sakazakii isolates from Xinjiang were found to be less than 0.083, which were negative for enterotoxin, but were observed by feeding experiment and analyzed by pathological section. It was found that Enterobacter sakazakii isolates from Xinjiang can still cause growth retardation and organ damage in young mice, and the damage is systemic, including important organs such as intestine, liver, spleen, kidney and so on. Conclusion: Enterobacter sakazakii is a bacterium with no significant drug resistance in Enterobacteriaceae, and Enterobacter sakazakii isolates from Xinjiang have potential harm to organism.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R378

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 馬洪波;馮子力;譚華;;菌(毒)種保存及復(fù)蘇技術(shù)[J];中國(guó)國(guó)境衛(wèi)生檢疫雜志;2006年04期

2 裴曉燕;劉秀梅;;阪崎腸桿菌檢測(cè)方法的研究進(jìn)展[J];國(guó)外醫(yī)學(xué)(衛(wèi)生學(xué)分冊(cè));2006年06期

3 周庭銀，馬子行;從三例患者重復(fù)分離出坂崎腸桿菌[J];臨床檢驗(yàn)雜志;1994年S1期

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本文編號(hào)：1971542