發(fā)布時(shí)間：2018-06-01 05:30

  本文選題：注意缺陷多動(dòng)障礙 + 遠(yuǎn)志��； 參考：《中醫(yī)雜志》2017年20期

【摘要】：目的探討遠(yuǎn)志-石菖蒲藥對(duì)治療注意缺陷多動(dòng)障礙(ADHD)的可能作用機(jī)制。方法將30只自發(fā)性高血壓(SHR)大鼠隨機(jī)分為模型組、陽(yáng)性對(duì)照組、遠(yuǎn)志-石菖蒲組,每組10只,另設(shè)WKY大鼠10只為對(duì)照組。遠(yuǎn)志-石菖蒲組給予遠(yuǎn)志-石菖蒲混懸液4.76 mg/(kg·d)灌胃;陽(yáng)性對(duì)照組給予利他林混懸液2mg/(kg·d)灌胃;正常組和模型組均以0.5%羧甲基纖維素鈉溶液2 ml/(kg·d)灌胃,在大鼠夜周期9:30~10:30、15:30~16:30分兩次灌胃,連續(xù)給藥4周。分別于給藥前、給藥4周及停藥2周時(shí),采用開(kāi)場(chǎng)實(shí)驗(yàn)評(píng)價(jià)大鼠自發(fā)性行為;給藥4周采用高架十字迷宮、新物體識(shí)別實(shí)驗(yàn)評(píng)估大鼠沖動(dòng)行為及學(xué)習(xí)記憶功能。停藥2周時(shí)檢測(cè)前額葉皮質(zhì)中多巴胺(DA)、多巴胺轉(zhuǎn)運(yùn)體(DAT)水平。結(jié)果給藥4周遠(yuǎn)志-石菖蒲組大鼠在開(kāi)場(chǎng)實(shí)驗(yàn)中的總運(yùn)動(dòng)距離及直立次數(shù)、進(jìn)入高架十字迷宮開(kāi)臂次數(shù)百分比和開(kāi)臂滯留時(shí)間百分比均低于模型組(P0.05),而在新物體識(shí)別實(shí)驗(yàn)對(duì)新物體的偏好指數(shù)則高于模型組(P0.05);停藥2周遠(yuǎn)志-石菖蒲組大鼠總運(yùn)動(dòng)距離及直立次數(shù)仍少于模型組,前額葉皮質(zhì)中DA水平較模型組明顯升高(P0.05),而DAT含量與模型組間差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論遠(yuǎn)志-石菖蒲藥對(duì)可提高ADHD小鼠前額葉皮質(zhì)中DA含量,可能是其治療ADHD的作用機(jī)制之一。
[Abstract]:Objective to explore the possible mechanism of Radix Polygali-Acorus calamus on ADHD in the treatment of attention deficit hyperactivity disorder (ADHD). Methods Thirty spontaneously hypertensive SHRs were randomly divided into three groups: model group, positive control group, Rhizoma Polygala and Acorus calamus group with 10 rats in each group and 10 WKY rats as control group. The control group was given Ritalin suspension (2mg/(kg d), the normal group and the model group were given 0.5% sodium carboxymethyl cellulose solution for 2 ml/(kg d, and the rats were perfused twice at 9: 30: 30: 3015: 3016: 30 during the night period of 9: 30: 30: 3015: 30: 30: 30, respectively, and the rats in the model group were treated with 0.5% sodium carboxymethyl cellulose solution for 2 ml/(kg d. The drug was given continuously for 4 weeks. The spontaneous behavior of rats was evaluated by open field experiment before administration, 4 weeks before administration and 2 weeks after withdrawal, and by using elevated cross maze at 4 weeks. The impulsive behavior and learning and memory function of rats were evaluated by new object recognition experiment. At 2 weeks after withdrawal, the levels of dopamine cinnamon and dopamine transporter were detected in the prefrontal cortex. Results the total distance of movement and the number of times of standing up in the open field experiment of the rats in the group of Polygala acuminata and Acorus calamus were given for 4 weeks. The percentage of open arm times and open arm retention time in the elevated cross maze was lower than that in the model group, but the preference index of the new object recognition experiment was higher than that of the model group. The total movement distance and the number of upright times were still less than those of the model group. The DA level in the prefrontal cortex was significantly higher than that in the model group (P 0.05), but there was no significant difference between the DAT content and the model group (P 0.05). Conclusion Radix Polygala and Acorus calamus can increase DA content in prefrontal cortex of ADHD mice, which may be one of the mechanisms of its therapeutic effect on ADHD.
【作者單位】： 深圳市中醫(yī)院;深圳市老年醫(yī)學(xué)研究所;
【基金】：國(guó)家自然科學(xué)基金(81503616) 中國(guó)博士后科學(xué)基金(2016M590840) 廣東省省級(jí)科技計(jì)劃(2014A020221003) 廣東省中醫(yī)藥局科研項(xiàng)目(20161218) 深圳市科技計(jì)劃(JCYJ20160428105935612) 深圳市衛(wèi)生計(jì)生系統(tǒng)科研項(xiàng)目(201505019)
【分類(lèi)號(hào)】：R285.5;R-332

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