發(fā)布時(shí)間：2018-05-26 12:22

  本文選題：ZNF580 + H_2O_2�。� 參考：《河北醫(yī)科大學(xué)》2011年碩士論文

【摘要】：ZNF580是由本課題組克隆的一種新的鋅指蛋白基因,該基因定位于人19號(hào)染色體19q13.42,最初是以LDL誘導(dǎo)人臍靜脈內(nèi)皮細(xì)胞系ECV304,篩選人主動(dòng)脈cDNA文庫(kù)得到的新基因(Genbank注冊(cè)號(hào):AF184939),國(guó)際人類(lèi)基因命名委員會(huì)命名為ZNF580。ZNF580與Krüppel樣鋅指轉(zhuǎn)錄因子(Krüppel-like factors, Klfs)家族成員類(lèi)似,其功能仍在進(jìn)一步研究中,本實(shí)驗(yàn)旨在探討ZNF580在ROS介導(dǎo)的內(nèi)皮細(xì)胞炎癥反應(yīng)中的信號(hào)轉(zhuǎn)導(dǎo)機(jī)制。 目的: 以人臍靜脈內(nèi)皮細(xì)胞株(EA.hy926)為體外模型,在典型的活性氧(reactive oxygen species, ROS)——H_2O_2的作用下,研究人C2H2型鋅脂蛋白新基因ZNF580表達(dá)情況,分析ZNF580基因表達(dá)與氧化應(yīng)激相關(guān)的核轉(zhuǎn)錄因子NF-κB轉(zhuǎn)錄激活之間的關(guān)系,以及ZNF580基因過(guò)表達(dá)對(duì)炎癥因子IL-8的釋放和單核細(xì)胞遷移趨化的影響,進(jìn)一步揭示ZNF580在ROS介導(dǎo)的內(nèi)皮細(xì)胞炎癥反應(yīng)中的作用。 方法: 1人臍靜脈內(nèi)皮細(xì)胞株(EA.hy926),用含10%胎牛血清的高糖DMEM培養(yǎng)基在37℃、5% CO_2、飽和濕度下培養(yǎng)。 2 H_2O_2是一個(gè)細(xì)胞損傷的刺激因素,而LDH(乳酸脫氫酶)是反映細(xì)胞膜完整性的重要檢測(cè)指標(biāo)。為確定H_2O_2對(duì)內(nèi)皮細(xì)胞的損傷情況,不同濃度H_2O_2(0-600μM)作用于EA.hy926細(xì)胞6h后,提取細(xì)胞上清,采用LDH活性檢測(cè)分析H_2O_2對(duì)內(nèi)皮細(xì)胞的損傷作用。 3胰酶消化收集對(duì)數(shù)生長(zhǎng)期EA.hy926細(xì)胞,將其懸浮于高糖DMEM細(xì)胞培養(yǎng)液中,濃度約為5-6×106/ml,接種到六孔板中2 ml/孔,37℃,5%CO_2孵箱培養(yǎng)。在各孔內(nèi)加H_2O_2 0μM、50μM、100μM、200μM、300μM、400μM,每組設(shè)三個(gè)平行孔。1h后收集細(xì)胞,提取總RNA和蛋白,利用半定量RT-PCR、Western blot方法檢測(cè)不同濃度H_2O_2對(duì)ZNF580表達(dá)的影響。 4在各孔中加入濃度為100μM的H_2O_2,每組設(shè)三個(gè)平行孔,于0h、0.5h、1h、2h、4h、6h后收集細(xì)胞,提取總RNA和蛋白,同樣利用半定量RT-PCR、Western blot方法檢測(cè)不同作用時(shí)間的H_2O_2對(duì)ZNF580表達(dá)的影響。 5內(nèi)皮細(xì)胞經(jīng)不同處理后,實(shí)驗(yàn)分為對(duì)照組、H_2O_2處理組、PDTC干預(yù)組和DMSO對(duì)照組。各組細(xì)胞爬片后固定,免疫細(xì)胞化學(xué)檢測(cè)H_2O_2和特異性抑制劑PDTC對(duì)NF-κB亞基p65激活表達(dá)的影響。 6同樣EA.hy926細(xì)胞分為不同的處理方式,抽提RNA以及蛋白,利用半定量RT-PCR擴(kuò)增和Western blot方法檢測(cè)H_2O_2是否通過(guò)NF-κB信號(hào)通路影響和調(diào)節(jié)ZNF580的表達(dá)。 7利用pEGFP-ZNF580和pEGFP-C1瞬時(shí)轉(zhuǎn)染EA.hy926內(nèi)皮細(xì)胞,獲得瞬時(shí)過(guò)表達(dá)ZNF580的EA.hy926細(xì)胞。運(yùn)用倒置熒光顯微鏡觀察轉(zhuǎn)染效率,RT-PCR以及western-blot技術(shù)鑒定轉(zhuǎn)染后ZNF580的表達(dá)情況。 8運(yùn)用real-time PCR技術(shù)從轉(zhuǎn)錄水平檢測(cè)構(gòu)建的瞬時(shí)高表達(dá)ZNF580內(nèi)皮細(xì)胞和正常內(nèi)皮細(xì)胞中白介素-8的表達(dá)差異。以ELISA方法從蛋白表達(dá)水平檢測(cè)構(gòu)建的瞬時(shí)高表達(dá)ZNF580內(nèi)皮細(xì)胞和正常內(nèi)皮細(xì)胞中白介素-8的表達(dá)差異。 9人急性單核細(xì)胞白血病細(xì)胞株(THP-1),用含10%胎牛血清的RPMI-1640培養(yǎng)基在37℃、5% CO_2、飽和濕度下培養(yǎng)。遷移趨化實(shí)驗(yàn)分析瞬時(shí)高表達(dá)ZNF580內(nèi)皮細(xì)胞和正常內(nèi)皮細(xì)胞中IL-8對(duì)單核細(xì)胞株THP-1遷移率的影響。 結(jié)果: 1不同濃度H_2O_2作用于EA.hy926細(xì)胞, LDH測(cè)定結(jié)果顯示,H_2O_2濃度為400μM時(shí),LDH的釋放量最大。 2不同濃度H_2O_2作用EA.hy926內(nèi)皮細(xì)胞1h后,提取RNA擴(kuò)增ZNF580片段,以及western-blot觀察ZNF580的蛋白表達(dá)水平,結(jié)果證實(shí),ZNF580在mRNA轉(zhuǎn)錄水平和蛋白水平的表達(dá)均有增加,并呈現(xiàn)濃度依賴(lài)的趨勢(shì)。(P0.05)。在濃度為100μM時(shí),ZNF580的表達(dá)最高。 3濃度為100μM的H_2O_2作用EA.hy926內(nèi)皮細(xì)胞不同時(shí)間后,由RT-PCR和western-blot檢測(cè)可知ZNF580 mRNA和蛋白水平的表達(dá)增加(P0.05)。在刺激時(shí)間為1h時(shí),ZNF580的表達(dá)最高。 4 H_2O_2作用可激活NF-κB亞基p65由細(xì)胞質(zhì)轉(zhuǎn)移至細(xì)胞核,而特異性抑制劑PDTC存在時(shí),可抑制NF-κB亞基p65在EA.hy926細(xì)胞內(nèi)的核轉(zhuǎn)位。陰性對(duì)照DMSO未引起p65核轉(zhuǎn)位。對(duì)照組可見(jiàn)棕色顆粒位于細(xì)胞質(zhì)中,細(xì)胞核中未見(jiàn)。 5 NF-κB信號(hào)通路抑制劑PDTC和H_2O_2共同處理可抑制ZNF580 mRNA及蛋白的上調(diào)表達(dá),而單獨(dú)H_2O_2的處理可明顯上調(diào)ZNF580 mRNA及蛋白的表達(dá)。 6脂質(zhì)體瞬時(shí)轉(zhuǎn)染ZNF580質(zhì)粒于EA.hy926細(xì)胞中,成功構(gòu)建瞬時(shí)高表達(dá)ZNF580的EA.hy926細(xì)熒光顯微鏡鑒定轉(zhuǎn)染效率為50%左右,RT-PCR和western-blot鑒定結(jié)果顯示:與轉(zhuǎn)染pEGFP-C1的細(xì)胞相比,轉(zhuǎn)染pEGFP-ZNF580的細(xì)胞ZNF580表達(dá)量顯著升高(P0.01)。 7 Real-time PCR及ELISA分析結(jié)果表明,高表達(dá)ZNF580的EA.hy926細(xì)胞中IL-8的mRNA轉(zhuǎn)錄和蛋白表達(dá)顯著增高。高表達(dá)ZNF580的EA.hy926細(xì)胞表達(dá)的IL-8顯著高于正常細(xì)胞(P0.01)。 8 Transwell實(shí)驗(yàn)檢測(cè)分析結(jié)果表明,高表達(dá)ZNF580的EA.hy926細(xì)胞對(duì)單核細(xì)胞THP-1遷移趨化作用有明顯的促進(jìn)作用,與正常組比較結(jié)果有統(tǒng)計(jì)學(xué)意義(P0.01)。 結(jié)論: H_2O_2通過(guò)NF-κB信號(hào)通路影響ZNF580的表達(dá),進(jìn)一步促進(jìn)其下游的炎癥因子IL-8的釋放,ZNF580在內(nèi)皮細(xì)胞炎癥反應(yīng)中起著重要的作用。該研究闡明了一條與炎癥發(fā)生相關(guān)的信號(hào)傳導(dǎo)途徑,為探討人C2H2型鋅脂蛋白新基因ZNF580功能及其與炎癥發(fā)生之間的關(guān)系提供科學(xué)依據(jù)。
[Abstract]:ZNF580 is a new zinc finger protein gene cloned by our group. This gene is located on human chromosome 19 19q13.42. It was originally a new gene (Genbank registration number: AF184939) obtained by LDL induction of human umbilical vein endothelial cell line ECV304 (Genbank registration number: AF184939). The national human gene naming Committee was named ZNF580.ZNF580 and Kr u. The ppel like zinc finger transcription factor (Kr u ppel-like factors, Klfs) family is similar, and its function is still in further study. The aim of this experiment is to explore the signal transduction mechanism of ZNF580 in ROS mediated endothelial cell inflammatory response.
Objective:
The human umbilical vein endothelial cell line (EA.hy926) was used as an in vitro model to study the expression of ZNF580 in human C2H2 type zinc lipoprotein (reactive oxygen species, ROS), H_2O_2, and to analyze the relationship between the ZNF580 gene expression and the activation of the nuclear transcription factor NF- kappa B transcription related to oxidative stress, as well as ZNF580. The effect of gene overexpression on the release of inflammatory factor IL-8 and the migration and chemotaxis of monocyte, and further reveal the role of ZNF580 in ROS mediated endothelial cell inflammatory response.
Method:
1 human umbilical vein endothelial cell line (EA.hy926) was cultured with high glucose DMEM medium containing 10% fetal bovine serum at 37 degree, 5% CO_2, and saturated humidity.
2 H_2O_2 is a stimulating factor of cell damage, and LDH (lactate dehydrogenase) is an important indicator to reflect the integrity of cell membrane. In order to determine the damage of H_2O_2 to endothelial cells, different concentrations of H_2O_2 (0-600 mu M) act on EA.hy926 cell 6h, extract cell supernatant, and use LDH activity to detect the damage of H_2O_2 to endothelial cells. Use.
3 trypsin digestion and collection of EA.hy926 cells in the logarithmic growth period were suspended in high glucose DMEM cell culture solution, the concentration was about 5-6 x 106/ml, inoculated to 2 ml/ holes in the six pore plate, 37 C and incubated in 5%CO_2 incubator. The cells were added in each hole with H_2O_2 0 mu M, 50 Mu M, 100 u M, 200 mu M, 300 mu M, 400 mu M, and each set of three parallel holes collected cells and extracted total proteins and proteins. Semi quantitative RT-PCR and Western blot were used to detect the effect of different concentrations of H_2O_2 on ZNF580 expression.
4 H_2O_2 with a concentration of 100 M was added to each hole. Each group was set up with three parallel holes. After 0h, 0.5h, 1H, 2h, 4h, and 6h, the cells were collected, and the total RNA and protein were extracted.
5 the endothelial cells were treated with different treatments, including the control group, the H_2O_2 treatment group, the PDTC intervention group and the DMSO control group. The cells were fixed in each group after climbing the tablet. The effects of H_2O_2 and specific inhibitor PDTC on the activation and expression of NF- kappa B subunit p65 were detected by immunocytochemistry.
6 the same EA.hy926 cells were divided into different treatments, RNA and protein were extracted. Semi quantitative RT-PCR amplification and Western blot were used to detect whether H_2O_2 could affect and regulate the expression of ZNF580 through the NF- kappa B signaling pathway.
7 transient transfection of EA.hy926 endothelial cells by pEGFP-ZNF580 and pEGFP-C1 was used to obtain transient EA.hy926 cells expressing ZNF580. The transfection efficiency was observed by inverted fluorescence microscopy, and the expression of ZNF580 was identified by RT-PCR and Western-blot technology.
8 real-time PCR technique was used to detect the difference in the expression of interleukin -8 in transient high expression ZNF580 endothelial cells and normal endothelial cells from transcriptional level. The difference in the expression of interleukin -8 in transient high expression ZNF580 endothelial cells and normal endothelial cells was detected by ELISA method from protein expression level.
9 people with acute monocytic leukemia cell line (THP-1) were cultured with RPMI-1640 medium containing 10% fetal bovine serum at 37, 5% CO_2 and saturated humidity. Migration and chemotaxis experiments were conducted to analyze the effect of IL-8 on the mobility of THP-1 in the mononuclear cell line of transient high expression of ZNF580 endothelial cells and normal endothelial cells.
Result:
1 different concentrations of H_2O_2 acted on EA.hy926 cells. The results of LDH showed that LDH release was the largest when H_2O_2 concentration was 400 M.
2 after EA.hy926 endothelial cell 1H was treated with different concentrations of H_2O_2, the ZNF580 fragment was amplified by RNA and the protein expression level of ZNF580 was observed by Western-blot. The results showed that the expression of ZNF580 at mRNA transcriptional level and protein level increased, and showed a tendency of concentration dependence. (P0.05), the expression of ZNF580 was the highest when the concentration was 100 mu M.
The expression of ZNF580 mRNA and protein was increased by RT-PCR and Western-blot (P0.05) after 3 EA.hy926 endothelial cells with a concentration of 100 M, and the expression of ZNF580 mRNA and protein was increased (P0.05). The expression of ZNF580 was the highest when the time of stimulation was 1H.
The effect of 4 H_2O_2 can activate the NF- kappa B subunit p65 from the cytoplasm to the nucleus, while the specific inhibitor PDTC can inhibit the nuclear transposition of NF- kappa B subunit p65 in the EA.hy926 cell. Negative control DMSO does not cause the p65 nucleus transposition. The control group can not be found in the cytoplasm of the cytoplasm and the nucleus of the cell nucleus.
5 NF- kappa B signaling pathway inhibitor, PDTC and H_2O_2, can inhibit the up-regulated expression of ZNF580 mRNA and protein, while H_2O_2 alone can obviously increase the expression of ZNF580 mRNA and protein.
6 liposomes were transiently transfected into the ZNF580 plasmid in EA.hy926 cells. The EA.hy926 fine fluorescence microscope was successfully constructed to identify the transient high expression ZNF580. The transfection efficiency was about 50%. The results of RT-PCR and Western-blot identification showed that the expression of pEGFP-ZNF580 in pEGFP-ZNF580 was significantly higher than that of the transfected pEGFP-C1 cells (P0.01).
The results of 7 Real-time PCR and ELISA analysis showed that the mRNA transcriptional and protein expression of IL-8 in the EA.hy926 cells with high expression of ZNF580 was significantly higher. The IL-8 of EA.hy926 cells expressing ZNF580 was significantly higher than that of normal cells (P0.01).
The results of 8 Transwell test showed that the EA.hy926 cells with high expression of ZNF580 had a significant effect on the chemotaxis of THP-1 migration in mononuclear cells, and the results were statistically significant (P0.01) compared with the normal group.
Conclusion:
H_2O_2 affects the expression of ZNF580 through the NF- kappa B signaling pathway and further promotes the release of the inflammatory factor IL-8 in the lower reaches. ZNF580 plays an important role in the inflammatory response of endothelial cells. This study elucidated a signal transduction pathway associated with the occurrence of inflammation, in order to explore the ZNF580 function of human C2H2 type zinc lipoprotein (znlipoprotein) and its inflammation and inflammation. The relationship between occurrence provides a scientific basis.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R363

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