本文選題：結(jié)核菌 + 結(jié)核休眠菌�。� 參考：《上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2016年11期

【摘要】：目的·利用缺鉀培養(yǎng)誘導(dǎo)結(jié)核菌進(jìn)入休眠狀態(tài)。方法·采用缺鉀Sauton培養(yǎng)基培養(yǎng)對(duì)數(shù)生長期結(jié)核菌,誘導(dǎo)其進(jìn)入休眠狀態(tài)采用菌落計(jì)數(shù)、刃天青試驗(yàn)和最大或然數(shù)法(MPN)檢測結(jié)核菌存活率及休眠菌比例;采用透射電子顯微鏡觀察結(jié)核菌細(xì)胞壁增厚情況;用金胺O-尼羅紅雙熒光染色法檢測結(jié)核菌脂質(zhì)聚集及失去抗酸性的情況;藥敏實(shí)驗(yàn)檢測結(jié)核菌對(duì)異煙肼和利福平的耐藥情況。結(jié)果·缺鉀培養(yǎng)30d后,結(jié)核菌發(fā)生呈球形、細(xì)胞壁增厚、脂質(zhì)聚集、失去抗酸性及對(duì)抗結(jié)核藥物耐藥等改變。結(jié)論·缺鉀培養(yǎng)可誘導(dǎo)對(duì)數(shù)期結(jié)核菌進(jìn)入休眠狀態(tài)。
[Abstract]:Objective to induce Mycobacterium tuberculosis into dormancy by potassium deficiency culture. Methods the tuberculous bacteria in logarithmic growth stage were cultured on potassium deficiency Sauton medium, and were induced into dormancy state by colony counting. The survival rate and dormancy ratio of tuberculous bacteria were detected by edge azure test and maximum probability method. The thickening of the cell wall of tuberculous bacteria was observed by transmission electron microscope, the accumulation of lipid and the loss of acid resistance of tuberculous bacteria were detected by the double fluorescence staining method of O- Nile red, and the drug resistance of Mycobacterium tuberculosis to isoniazid and rifampicin was detected by drug sensitivity test. Results after 30 days of potassium deficiency culture, tuberculous bacteria appeared spherical, cell wall thickened, lipid accumulation, loss of acid resistance and anti-tuberculosis drug resistance and other changes. Conclusion potassium deficiency culture can induce mycobacterium tuberculosis to dormant in logarithmic phase.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院呼吸與危重癥醫(yī)學(xué)科;
【基金】：國家自然科學(xué)基金項(xiàng)目(81570010) 國家臨床重點(diǎn)�？茖ｍ�(xiàng)(2012-649)~~
【分類號(hào)】：R378.911

【相似文獻(xiàn)】

相關(guān)期刊論文 前3條

1 程素琦;張效良;何子安;吳孟平;陳西京;朱素英;李慧琴;張更聚;;熱環(huán)境下缺鉀的影響及鉀需要量的探討[J];人民軍醫(yī);1988年05期

2 李汝紅;缺鉀家兔輸液后體鉀變化的實(shí)驗(yàn)研究（摘要）[J];昆明醫(yī)學(xué)院學(xué)報(bào);1994年01期

3 ;[J];;年期

，

本文編號(hào)：1935557