發(fā)布時間：2018-05-25 15:33

  本文選題：重組抗原鐵蛋白 + 樹突狀細胞��； 參考：《四川大學(xué)學(xué)報(醫(yī)學(xué)版)》2017年06期

【摘要】：目的探索細粒棘球絳蟲重組抗原鐵蛋白(rEg.ferritin)免疫小鼠產(chǎn)生抵抗原頭蚴感染的保護機制。方法利用rEg.ferritin和囊液(HF)兩種細粒棘球絳蟲抗原與小鼠骨髓樹突狀細胞(BMDC)進行體外培養(yǎng),共分6組:HF組(加入HF 30μg/mL)、rEg.ferritin組(加入抗原rEg.ferritin 1μg/mL)均為單獨刺激組,rEg.ferritin+LPS組(加入抗原rEg.ferritin 1μg/mL和LPS 100ng/mL)和HF+LPS組(加入HF 30μg/mL和LPS 100ng/mL)為聯(lián)合刺激組,LPS陽性對照組(加入LPS 100ng/mL),空白對照組(不加任何抗原)。通過電鏡檢測各組BMDC形態(tài)學(xué)的變化;流式細胞技術(shù)檢測各組DC的表面分子表達、吞噬能力及誘導(dǎo)T細胞增殖能力的變化;酶聯(lián)免疫吸附試驗(ELISA)檢測各組BMDC培養(yǎng)上清液中所含Th1型[白介素(IL)-12p70,腫瘤壞死因子-α(TNF-α)]和Th2型(IL-6和IL-10)細胞因子含量。結(jié)果 rEg.ferritin組和rEg.ferritin+LPS組BMDC具有成熟的形態(tài),表面突起數(shù)目高于空白對照組、HF組和HF+LPS組(P0.05);高表達MHCⅡ、CD80、CD86及CD40分子,與空白對照組比較,差異有統(tǒng)計學(xué)意義(P0.05);誘導(dǎo)T細胞增殖能力強于空白對照組、HF組和HF+LPS組(P0.05),吞噬能力弱于HF組;高表達IL-6、IL-12p70、TNF-α及IL-10,與空白對照組比較,差異有統(tǒng)計學(xué)意義(P0.05)。結(jié)論 rEg.ferritin可以刺激BMDC細胞成熟,促進T增殖,釋放高含量的Th1型及Th2型細胞因子,激活Th1型或Th2型細胞,引發(fā)免疫應(yīng)答。
[Abstract]:Objective to explore the protective mechanism of Echinococcus granulosus recombinant antigen ferritin (rEg.ferritin) immunized mice against the infection of the Echinococcus granulosus. Methods using rEg.ferritin and HF two species of Echinococcus granulosus antigen and mouse bone marrow dendritic cells (BMDC) in vitro, they were divided into 6 groups: HF group (adding HF 30 mu g/mL), rEg.ferritin group (plus rEg.ferritin) Antigen rEg.ferritin 1 mu g/mL) was a single stimulation group, group rEg.ferritin+LPS (adding antigen rEg.ferritin 1 g/mL and LPS 100ng/mL) and HF+LPS group (HF 30 mu g/mL and LPS 100ng/mL) were combined stimulation group. Changes in the surface molecular expression of DC, phagocytosis and the ability to induce the proliferation of T cells by flow cytometry; enzyme linked immunosorbent assay (ELISA) to detect the Th1 type of Th1 [IL) -12p70, tumor necrosis factor - alpha (TNF- a)] and Th2 type (IL-6 and IL-10) cytokines in the supernatant of BMDC culture. The number of BMDC in group itin and group rEg.ferritin+LPS was mature, and the number of surface protuberances was higher than that of blank control group, HF group and HF+LPS group (P0.05). The high expression of MHC II, CD80, CD86 and CD40 molecules, compared with the blank control group, was statistically significant (P0.05), and the ability of inducing T cells to proliferate was stronger than that in the blank control group. The strength of IL-6, IL-12p70, TNF- A and IL-10 was higher than that of the HF group. The difference was statistically significant (P0.05) compared with the blank control group (P0.05). Conclusion rEg.ferritin can stimulate the maturation of BMDC cells, promote the proliferation of T, release the high content of Th1 and Th2 cytokine, activate the Th1 type or the type of cell and trigger the immune response.
【作者單位】： 寧夏醫(yī)科大學(xué)基礎(chǔ)學(xué)院醫(yī)學(xué)遺傳學(xué)與細胞生物學(xué)教研室;寧夏醫(yī)科大學(xué)包蟲病重點實驗室;寧夏醫(yī)科大學(xué)科學(xué)技術(shù)研究中心;
【基金】：寧夏自然科學(xué)基金(No.NZ16208)資助
【分類號】：R392

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