非洲綠猴血液學(xué)和血清生化指標(biāo)的測(cè)定及分析
發(fā)布時(shí)間:2018-05-21 10:52
本文選題:非洲綠猴 + 血液學(xué)指標(biāo) ; 參考:《中國(guó)比較醫(yī)學(xué)雜志》2017年11期
【摘要】:目的檢測(cè)50只健康非洲綠猴血液學(xué)、血清生化指標(biāo),分析不同性別、不同年齡段(青幼年組:1~3歲,成年組:4~6歲)非洲綠猴的血液學(xué)、血清生化指標(biāo)的差異。方法應(yīng)用全自動(dòng)血細(xì)胞分析儀及血液生化分析儀分別測(cè)定清醒狀態(tài)下健康非洲綠猴血液學(xué)及血清生化指標(biāo)。結(jié)果血液學(xué)指標(biāo)中青幼年組與成年組差異顯著的指標(biāo)有紅細(xì)胞數(shù)(RBC)、血紅蛋白(HGB)、血細(xì)胞壓積(HCT)、中性粒細(xì)胞百分比(NEUT%)、淋巴細(xì)胞百分比(LYMPH%)、單核粒細(xì)胞百分比(MONO%)、嗜堿性粒細(xì)胞百分比(BASO%)(P0.05)。青幼年組中雌猴與雄猴差異顯著的指標(biāo)有白細(xì)胞數(shù)(WBC)、RBC、HGB、HCT、NEUT%、LYMPH%、MONO%(P0.05),成年組中雌猴與雄猴差異顯著的指標(biāo)有HCT(P0.05),其它指標(biāo)差異不顯著。血清生化指標(biāo)中青幼年組與成年組差異顯著的指標(biāo)有白蛋白(ALB)、天門(mén)冬氨酸氨基轉(zhuǎn)移酶(AST)、堿性磷酸酶(ALP)、肌酐(CREA)(P0.05),青幼年組雌猴與雄猴差異顯著的指標(biāo)有CREA(P0.05),成年組雌猴與雄猴差異顯著的指標(biāo)有總蛋白(TP)、膽固醇(CHOL)(P0.05),其它指標(biāo)差異不顯著。結(jié)論本文初步建立了非洲綠猴的常規(guī)生物學(xué)數(shù)據(jù),為評(píng)價(jià)其生物學(xué)特性及相關(guān)應(yīng)用提供一定的數(shù)據(jù)基礎(chǔ),可供參考。
[Abstract]:Objective to detect the hematology and serum biochemical indexes of 50 healthy African green monkeys, and to analyze the differences of hematology and serum biochemical indexes of African green monkeys of different sex and age (young and young group: 1 + 3 years old, adult group: 4 ~ 6 years old). Methods Hematology and serum biochemical indexes of healthy African green monkey were measured by automatic hematology analyzer and blood biochemistry analyzer respectively. Results there were significant differences in hematologic indexes between young and green groups, such as red blood cell count (RBC), hemoglobin (HGB), hematocrit (HCT), neutrophil percentage (neuto), lymphocyte percentage (LYMPH), mononuclear cell (mononuclear), basic granulocyte (basophil) and basophil (basic granulocyte). The significant differences between female and male monkeys were as follows: the white blood cell count (WBC) of RBC / HGBHGBHGBHGBHGBHGBHGBHGBRBBCNBBBCHGBHGBHGBHGBHBNBBCNBBBCHGB / HGBHGB / HGBHGB / HGB / HGBHGB / HGBC / HGBC / HGBB / HGBC / HGB / HGB / HGB / HGB / HGBHGB / HGBHGB@@ Serum biochemical indexes: Albumin ALBX, aspartate aminotransferase (ASTT), alkaline phosphatase (ALPN), creatinine (CREAEAA) P0.05 in serum, creatine in young and young females and adult monkeys, and adult female monkeys, respectively. There were significant differences in total protein TPN and cholesterol Choll (P0.05) between male and male monkeys, but there was no significant difference in other indexes. Conclusion the conventional biological data of African green monkey are established in this paper, which can be used as a reference for the evaluation of its biological characteristics and related applications.
【作者單位】: 軍事醫(yī)學(xué)科學(xué)院實(shí)驗(yàn)動(dòng)物中心;
【基金】:國(guó)家自然科學(xué)基金(編號(hào):31272514,31501908)
【分類(lèi)號(hào)】:R-33
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本文編號(hào):1918902
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