本文選題：Notch1 + Notch4　； 參考：《重慶醫(yī)科大學(xué)》2011年碩士論文

【摘要】：目的 探討小鼠胚胎發(fā)育過(guò)程早期造血相關(guān)組織中Notch1和Notch4信號(hào)的基因與蛋白的表達(dá)情況。 方法 采用PT-PCR和Western-blotting方法,對(duì)小鼠胚胎9.5天(E9.5)、E10.5、E11.5、E12.5胚胎的主動(dòng)脈-性腺-中腎(aorta-gonad-mesonephros , AGM)和胎肝Notch1和Notch4信號(hào)基因和蛋白的表達(dá)進(jìn)行檢測(cè)。 結(jié)果 Notch1 mRNA在小鼠胚胎AGM區(qū)表達(dá)隨孕期延長(zhǎng)逐漸降低。Notch1 mRNA在胎肝E10.5表達(dá)最低,E11.5表達(dá)最高。Notch1蛋白與Notch1mRNA表達(dá)規(guī)律相似。Notch1 mRNA在AGM與胎肝各時(shí)間點(diǎn)比較,均具有顯著差異;Notch1蛋白表達(dá)除E9.5AGM區(qū)明顯高于胎肝外,余時(shí)間點(diǎn)表達(dá)無(wú)明顯差異。 AGM區(qū)Notch4 mRNA在E10.5表達(dá)最高;在胎肝中表達(dá)隨孕期延長(zhǎng)而降低。Notch4蛋白與Notch4 mRNA表達(dá)規(guī)律相似。Notch4 mRNA在AGM區(qū)與胎肝各時(shí)間點(diǎn)比較,在E9.5和E12.5有顯著差異,在E10.5與E11.5無(wú)明顯差異。 結(jié)論 Notch1和Notch4信號(hào)在小鼠胚胎早期造血組織中有表達(dá)。隨著胚胎發(fā)育,AGM區(qū)的造血能力下降,Notch1信號(hào)隨之減弱;相反,胎肝造血功能逐漸完善,Notch1信號(hào)逐漸增強(qiáng),提示Notch1信號(hào)可能與小鼠胚胎早期造血發(fā)育相關(guān)。AGM區(qū)Notch4信號(hào)在E10.5表達(dá)最高,其后逐漸下降;胎肝Notch4信號(hào)隨孕期延長(zhǎng)逐漸降低,提示Notch4信號(hào)可能與胚胎造血組織相關(guān)。
[Abstract]:Purpose To investigate the gene and protein expression of Notch1 and Notch4 signals in hematopoietic related tissues during early embryonic development in mice. Method The expression of Notch1 and Notch4 signal genes and proteins in aorta gonad-mesonephros (AGMs) and fetal liver were detected by PT-PCR and Western-blotting. Result The expression of Notch1 mRNA in the AGM region of mouse embryo decreased gradually with the prolongation of pregnancy. The lowest expression of Notch1 mRNA in fetal liver E10.5 the highest expression of Notch1 protein was similar to that of Notch1mRNA. The expression of Notch1 mRNA in AGM was compared with that in fetal liver at different time points. There were significant differences in the expression of Notch1 protein except in the E9.5AGM region, but there was no significant difference in the expression of Notch1 protein at other time points. The expression of Notch4 mRNA in AGM region was the highest in E10.5, and the expression of Notch4 protein in fetal liver was similar to that in Notch4 mRNA. The expression of Notch4 mRNA in AGM region was significantly different from that in fetal liver at each time point, but there was no significant difference between E9.5 and E11.5 in E10.5 and E11.5. Conclusion Notch1 and Notch4 signals were expressed in early embryonic hematopoietic tissues of mice. With the decline of hematopoiesis ability of AGM region in embryonic development, the signal of Notch1 decreased, on the contrary, the signal of fetal liver hematopoietic function was gradually enhanced, suggesting that Notch1 signal may be related to the early embryonic hematopoietic development of mice. The Notch4 signal of AGM region is the highest in E10.5. Notch4 signal of fetal liver decreased gradually with the prolongation of pregnancy, suggesting that Notch4 signal may be related to embryonic hematopoietic tissue.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R321

【共引文獻(xiàn)】

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