本文選題：新孢子蟲 + IFN-γ�。� 參考：《中國病原生物學雜志》2017年04期

【摘要】：目的探討新孢子蟲感染早期引起小鼠巨噬細胞IFN-γ、TNF-α和IL-6分泌的變化,分析其對宿主細胞Th1/Th2免疫應答的影響。方法分離純化新孢子蟲速殖子,刺激小鼠腹腔巨噬細胞,分別于6、12、18、24、36h收集培養(yǎng)上清,用ELISA方法檢測IFN-γ、TNF-α和IL-6分泌情況,分析蟲體感染對宿主細胞Th1/Th2免疫應答的影響。結果新孢子蟲感染小鼠巨噬細胞后IFN-γ的分泌量逐漸升高,前18h抑制IFN-γ的分泌,在18hIFN-γ分泌量約為450pg/ml;24h后IFN-γ分泌量明顯升高,在24h達到約1 200pg/ml。新孢子蟲感染后的36h內,TNF-α分泌量一直處于較高水平,均在1 200pg/ml以上;新孢子蟲感染后前18hIL-6分泌量逐漸增多,18h時達到峰值4 000pg/ml以上,24h后開始下降到約為650pg/ml。結論新孢子蟲感染早期小鼠巨噬細胞IFN-γ、TNF-α和IL-6的分泌呈現不同程度的變化。表明新孢子蟲通過影響IFN-γ和IL-6的分泌調控宿主細胞從Th2向Th1類型免疫應答轉變,感染后的前24h以Th2類型免疫應答為主,24h后向Th1類型免疫應答轉變。
[Abstract]:Objective to investigate the changes of IFN- 緯 TNF- 偽 and IL-6 secretion in murine macrophages during the early stage of neosporidium infection, and to analyze the effect of neosporidium infection on the immune response of host cells to Th1/Th2. Methods Mesosporidium tachyzoites were isolated and purified, and the peritoneal macrophages were stimulated. The supernatants were collected and cultured at 612A 18U 2436h. The secretion of IFN- 緯 TNF- 偽 and IL-6 was detected by ELISA method, and the effect of parasite infection on the immune response of host cells to Th1/Th2 was analyzed. Results the secretion of IFN- 緯 in macrophages of mice infected with neosporidium increased gradually, and the secretion of IFN- 緯 was inhibited at the first 18 hours. The secretion of IFN- 緯 increased significantly at 18h after about 450 PG / ml, and reached about 1 200 PG / ml at 24 h. The secretion of TNF- 偽 was at a higher level (above 1 200pg/ml) within 36 h after neosporidium infection, and the secretion of 18hIL-6 before neosporidium infection gradually increased to a peak of 4 000pg/ml or more than 24 h after neosporidium infection, and began to decrease to about 650 PG / ml for 24 h after neosporidium infection. Conclusion the secretion of IFN- 緯 -TNF- 偽 and IL-6 in murine macrophages at the early stage of neosporidium infection showed varying degrees. The results indicated that the host cells of neosporidium changed from Th2 to Th1 type by affecting the secretion of IFN- 緯 and IL-6, and the first 24 hours after infection, the main immune response was Th2 type, and the first 24 hours after infection, the host cells changed to Th1 type immune response.
【作者單位】： 吉林大學動物醫(yī)學學院;
【基金】：國家重點基礎研究發(fā)展計劃(973計劃)項目(No.2015CB150300)
【分類號】：R382.3

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本文編號：1900009