本文選題：干細(xì)胞 + 細(xì)胞增殖�。� 參考：《中國(guó)組織工程研究》2017年09期

【摘要】：背景:外周血單個(gè)核細(xì)胞群中含有外周血干細(xì)胞,其體外增殖情況仍不清楚,體內(nèi)移植也缺乏良好的標(biāo)記物。目的:以EdU作為標(biāo)記物,觀察外周血單個(gè)核細(xì)胞在干細(xì)胞培養(yǎng)基中的增殖情況,以及探討EdU標(biāo)記外周血干細(xì)胞的可行性。方法:分離新西蘭兔外周血單個(gè)核細(xì)胞,在加入EdU的干細(xì)胞培養(yǎng)液中培養(yǎng)1-5 d;于不同的時(shí)間點(diǎn)觀察細(xì)胞形態(tài)和進(jìn)行細(xì)胞計(jì)數(shù);收獲細(xì)胞,進(jìn)行EdU染色,觀察EdU陽(yáng)性細(xì)胞并檢測(cè)陽(yáng)性率。結(jié)果與結(jié)論:(1)剛分離出的外周血單個(gè)核細(xì)胞為類圓形,輪廓清晰;經(jīng)過(guò)1 d培養(yǎng)時(shí),大部分細(xì)胞仍懸浮在培養(yǎng)液中,球形或類圓形,有細(xì)胞團(tuán)出現(xiàn),也見(jiàn)少量細(xì)胞散在貼壁,呈現(xiàn)三角形或多邊形;培養(yǎng)2 d時(shí),可見(jiàn)較多細(xì)胞碎片,大多數(shù)細(xì)胞仍為類圓形;培養(yǎng)3-5 d時(shí),細(xì)胞碎片增多,細(xì)胞團(tuán)體積變小,細(xì)胞密度明顯降低;(2)隨著培養(yǎng)時(shí)間的延長(zhǎng),細(xì)胞計(jì)數(shù)逐漸降低;(3)在培養(yǎng)1 d時(shí),有散在EdU紅色標(biāo)記細(xì)胞;在培養(yǎng)2 d時(shí),EdU紅色標(biāo)記細(xì)胞明顯增多,并在培養(yǎng)三四天時(shí)維持不明顯的變化;在培養(yǎng)5 d時(shí),EdU紅色標(biāo)記的細(xì)胞明顯減少;培養(yǎng)2 d時(shí),EdU陽(yáng)性細(xì)胞率最高,為(2.38±0.10)%;(4)結(jié)果表明,外周血單個(gè)核細(xì)胞群中增殖細(xì)胞率很低,EdU能標(biāo)記外周血單個(gè)核細(xì)胞中有增殖能力的細(xì)胞。
[Abstract]:Background: peripheral blood mononuclear cells (PBMC) contain peripheral blood stem cells (PBSCs). Aim: to observe the proliferation of peripheral blood mononuclear cells (PBMC) in stem cell culture medium using EdU as marker and to explore the feasibility of EdU labeling peripheral blood stem cells (PBSCs). Methods: the peripheral blood mononuclear cells (PBMC) of New Zealand rabbits were isolated and cultured in stem cell culture medium supplemented with EdU for 1-5 days. Cell morphology and cell count were observed at different time points. Cells were harvested and stained with EdU. EdU positive cells were observed and the positive rate was detected. Results and conclusion: the peripheral blood mononuclear cells (PBMC) isolated from the first day of culture were round and clear, and most of the cells were still suspended in the culture medium after 1 day culture, the cells were round or round, there were cell clusters, and a few cells were scattered in the adherent to the wall. After 2 days of culture, more cell fragments were found, and most of the cells were still round. After 3 to 5 days of culture, the number of cell fragments increased, the cell mass became smaller, and the cell density decreased significantly with the prolongation of culture time. The number of EdU red labeled cells was decreased gradually at 1 day after culture, and the number of red labeled cells increased significantly at 2 days, and maintained no obvious change at 3 or 4 days after culture. The positive rate of Edu was the highest at 2 days (2.38 鹵0.10). The proliferation rate of peripheral blood mononuclear cells was very low and Edu could label the proliferative cells in peripheral blood mononuclear cells.
【作者單位】： 中山大學(xué)中山眼科中心眼科學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：廣東省科技計(jì)劃項(xiàng)目(2013B040200020,2013B020400003) 廣州市科技計(jì)劃項(xiàng)目(15570001)~~
【分類號(hào)】：R329.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前2條

1 姚芳芳;王效科;馮兆忠;鄭飛翔;馮宗煒;歐陽(yáng)志云;;EDU對(duì)臭氧脅迫下菠菜急性傷害癥狀和光合特性的影響[J];生態(tài)環(huán)境;2007年05期

2 ;[J];;年期

，

本文編號(hào)：1899707