本文選題：黃芪有效部位 + 失血性貧血��； 參考：《遼寧中醫(yī)藥大學(xué)》2012年碩士論文

【摘要】：目的： 通過借助失血性貧血小鼠模型,觀察黃芪及其不同有效部位對失血性貧血的干預(yù)作用,探討黃芪不同有效部位補血的差異性,并初步探討其作用機制。 材料與方法： 健康SPF級BALB/C小鼠132只,雌雄各半。所有小鼠經(jīng)眶靜脈采血測量小鼠外周血紅蛋白濃度,根據(jù)外周血紅蛋白濃度隨機將小鼠分為正常組、模型組、中藥對照組、黃芪組、酮糖苷組、糖苷組、酮糖組、酮苷組、多糖組、黃酮組、皂苷組,每組12只,雌雄各半。常規(guī)適應(yīng)性飼養(yǎng)3d后進行造模：除正常組外,各組小鼠分別于第1d、4d、7d進行眶靜脈放血,放血量為0.5ml/20g小鼠(即25ml/kg),造模第7d測小鼠血紅蛋白濃度較前減少20%為造模成功。造模第2d,正常組、模型組給予蒸餾水灌胃,各給藥組分別灌胃相應(yīng)藥物,均按1ml/100g體重劑量給藥,連續(xù)灌胃14d。末次灌胃24h后取材,檢測小鼠外周血紅細(xì)胞計數(shù)(RBC)、血紅蛋白濃度(HB)、紅細(xì)胞壓積(Hct), ELISA法測定實驗小鼠骨髓干細(xì)胞因子(SCF)濃度、血清特異轉(zhuǎn)錄因子(GATA-1)濃度、血清及腎組織勻漿液紅細(xì)胞生成素(EPO)濃度。 結(jié)果： 1.模型組小鼠RBC、HB以及Hct均明顯降低,表明失血性貧血動物模型成功建立。 2.一般狀態(tài)：各給藥治療組對失血性貧血所引起的小鼠乏力倦臥、扎堆、體型消瘦、飲食減少等癥狀均能改善。 3.外周血象改變：與模型組比較,各給藥組均能明顯升高實驗小鼠HB、RBC、Hct,糖苷組能夠明顯升高實驗小鼠外周HB濃度,差異有統(tǒng)計學(xué)意義(P0.05)。 4.與模型組比較,除皂苷組外各給藥組均較模型組小鼠血清GATA-1濃度、骨髓SCF濃度明顯升高,黃酮組均優(yōu)于酮糖苷組、糖苷組、酮糖組、酮苷組、多糖組,黃芪組均明顯優(yōu)于酮糖苷組、多糖組,但酮糖組在升高血清GATA-1濃度方面優(yōu)于多糖組,黃芪組在升高骨髓SCF濃度方面優(yōu)于糖苷組,差異有統(tǒng)計學(xué)意義(P0.05)。 5.與模型組比較,黃芪組、酮糖苷組、糖苷組、黃酮組明顯升高小鼠腎組織勻漿EPO濃度,其中尤以糖苷組效用最佳,差異有統(tǒng)計學(xué)意義(P0.05)：與模型組比較,除皂苷組外各給藥組均明顯升高小鼠血清EPO濃度,其中黃芪組效用最佳,差異有統(tǒng)計學(xué)意義(P0.05),酮糖、糖苷配伍均優(yōu)于單一組分用藥。 結(jié)論： 1.黃芪及其不同有效部位對失血性貧血均具有糾正作用。 2.黃芪及其不同有效部位均可增加失血性貧血小鼠HB、RBC及Hct,多糖+皂苷可能是改善失血性貧血小鼠外周血象的最佳有效配伍組合。 3.除皂苷組外黃芪及其有效部位均可通過促進小鼠骨髓SCF、血GATA-1、血EPO、腎組織EPO濃度的升高來實現(xiàn)其補血作用,黃酮增加骨髓SCF、血GATA-1作用最佳,多糖+皂苷升高血清及腎組織EPO效果最佳。
[Abstract]:Objective: Through observing the intervention effect of astragalus and its effective parts on hemorrhagic anemia with the help of hemorrhagic anemia mouse model, the difference of reinforcing blood in different effective parts of Astragalus membranaceus was discussed, and the mechanism of its action was preliminarily discussed. Materials and methods: There were 132 healthy BALB/C mice of SPF grade, half male and half female. The peripheral hemoglobin concentration of all mice was measured by orbital vein. According to the peripheral hemoglobin concentration, mice were randomly divided into normal group, model group, Chinese medicine control group, astragalus group, ketoglucoside group, ketosugar group, ketoside group, and ketoside group. Polysaccharide group, flavonoid group, saponins group, 12 rats in each group, half male and female. After 3 days of conventional adaptive feeding, the mice in each group were treated with orbital vein bleeding on day 1 and day 4, respectively, except the normal group. The volume of blood was 25 ml / kg in 0.5ml/20g mice, and the hemoglobin concentration of mice was decreased by 20% on the 7th day after the model was established. The results showed that the hemoglobin concentration of the mice decreased by 20% on the 7th day. On the 2nd day, the normal group and the model group were given distilled water by gavage, and each group was given the corresponding drug by stomach respectively, which was given by 1ml/100g body weight for 14 days. The samples were collected 24 hours after the last gastric administration. The red blood cell count (RBC), hemoglobin concentration (HBN), hematocrit (HCT), bone marrow stem cell factor (SCF) and serum specific transcription factor (GATA-1) were measured by ELISA. Erythropoietin (EPO) concentration in serum and renal homogenate. Results: 1. The HB and Hct of mice in the model group were significantly decreased, which indicated that the animal model of hemorrhagic anemia was established successfully. 2. General state: the symptoms of hemorrhagic anemia in each treatment group were improved, such as fatigue and sleepiness, mass, weight loss, diet reduction and so on. 3. The changes of peripheral blood image: compared with the model group, each administration group could significantly increase the HBB RBCU HCT in the experimental mice, and the glycoside group could significantly increase the peripheral HB concentration of the experimental mice, the difference being statistically significant (P 0.05). 4. Compared with the model group, the serum GATA-1 concentration and bone marrow SCF concentration in each administration group except the saponins group were significantly higher than those in the model group. The flavonoid group was superior to ketoglucoside group, ketoside group and polysaccharide group. Astragalus group was superior to ketoglucoside group and polysaccharide group, but ketosugar group was superior to polysaccharide group in raising serum GATA-1 concentration, Astragalus group was superior to glycoside group in raising bone marrow SCF concentration, the difference was statistically significant (P 0.05). 5. Compared with the model group, Astragalus membranaceus group, ketoglucoside group and flavonoid group significantly increased the concentration of EPO in the renal homogenate of mice, especially in the glycoside group, and the difference was statistically significant (P 0.05). The concentration of serum EPO was significantly increased in each administration group except the saponins group. The effect of astragalus membranaceus group was the best, and the difference was statistically significant (P 0.05). The compatibility of keto sugar and glycoside was better than that of single component. Conclusion: 1. Astragalus membranaceus and its different effective parts can correct hemorrhagic anemia. 2. Astragalus membranaceus and its different effective parts could increase HBG RBC and HCT in hemorrhagic anemia mice. Polysaccharide saponins might be the best combination to improve peripheral blood picture of hemorrhagic anemia mice. 3. In addition to saponins group, Astragalus membranaceus and its effective parts could enhance the concentration of bone marrow SCF, blood GATA-1, blood EPO, renal tissue EPO to achieve the effect of tonifying their blood. Flavonoids could increase bone marrow SCF, and blood GATA-1 had the best effect. The effect of polysaccharide saponins on serum and renal EPO was the best.
【學(xué)位授予單位】：遼寧中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R-332;R285.5

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