本文選題：椎間盤退化 + 腫瘤壞死因子α��； 參考：《中國(guó)組織工程研究》2017年16期

【摘要】：背景:六味地黃丸作為補(bǔ)益肝腎名方,臨床應(yīng)用其治療腰痛已取得良好的效果,而有關(guān)六味地黃丸治療腎虛型腰痛的作用機(jī)制尚不明確。目的:觀察六味地黃丸對(duì)兔椎間盤體外退變模型細(xì)胞外基質(zhì)組分的影響,探討六味地黃丸防治椎間盤退變的療效。方法:將20只新西蘭兔帶終板的L1-L6椎間盤100個(gè)隨機(jī)分為空白對(duì)照組、腫瘤壞死因子α組、p38-JNK/SAPK阻斷組、六味地黃丸組、腫瘤壞死因子α+六味地黃丸組,每組20個(gè)。腫瘤壞死因子α組培養(yǎng)液中含10 mg/L腫瘤壞死因子α2μL,p38-JNK/SAPK阻斷組培養(yǎng)液中含p38MAPK特異性阻斷劑SB203580,JNK特異性阻斷劑SP600125各20μmol/L 10μL,六味地黃丸組培養(yǎng)液中含體積分?jǐn)?shù)為10%六味地黃丸血清,腫瘤壞死因子α+六味地黃丸組含10 mg/L腫瘤壞死因子α2μL和體積分?jǐn)?shù)為10%六味地黃丸血清,分別于培養(yǎng)的第2,4,8,14天收集標(biāo)本待測(cè)。結(jié)果與結(jié)論:在培養(yǎng)基中加入腫瘤壞死因子α能明顯上調(diào)Ⅰ型膠原mRNA和蛋白的表達(dá),下調(diào)糖胺多糖含量、硫酸軟骨素/硫酸角質(zhì)素比值、透明質(zhì)酸含量,蛋白多糖mRNA和蛋白的表達(dá)、Ⅱ型膠原mRNA和蛋白的表達(dá)(P0.05),六味地黃丸含藥血清可部分對(duì)抗腫瘤壞死因子α所導(dǎo)致的改變,提示六味地黃丸可在一定程度上延緩了椎間盤退變。
[Abstract]:Background: as a famous prescription for tonifying liver and kidney, Liuwei Dihuang Pill has achieved good results in the treatment of low back pain, but the mechanism of Liuwei Dihuang Pill in treating low back pain of kidney deficiency type is not clear. Aim: to observe the effect of Liuwei Dihuang pill on extracellular matrix components of rabbit intervertebral disc degeneration model in vitro and to explore the effect of Liuwei Dihuang pill on the prevention and treatment of disc degeneration. Methods: 100 L1-L6 intervertebral discs with endplate in 20 New Zealand rabbits were randomly divided into control group (n = 20), tumor necrosis factor 偽 group (n = 20), and tumor necrosis factor 偽 group (n = 20). TNF- 偽 group contained 10 mg/L TNF- 偽 2 渭 L p38-JNK-SAPK blocking medium containing 10 渭 mol/L 10 渭 L p38MAPK specific blocker SB203580 and 10 渭 mol/L 10 渭 L SP600125, and the volume fraction of 10% Liuwei Dihuang pills was 10% Liuwei Dihuang Pill. Tumor necrosis factor 偽 Liuwei Dihuang pill group contained 10 mg/L tumor necrosis factor 偽 2 渭 L and volume fraction of 10% Liuwei Dihuang pill serum. Results and conclusion: tumor necrosis factor 偽 can significantly up-regulate the expression of type I collagen mRNA and protein, down-regulate the content of glycosaminoglycan, the ratio of chondroitin sulfate to keratin sulfate, and the content of hyaluronic acid. The expression of proteoglycan mRNA and protein, the expression of type 鈪，

本文編號(hào)：1887203