本文選題：干細胞 + 骨髓干細胞��； 參考：《中國組織工程研究》2017年13期

【摘要】：背景:研究發(fā)現(xiàn)降鈣素基因相關肽能夠增強成骨細胞的生物學活性,實驗擬進一步檢測降鈣素基因相關肽對骨髓間充質(zhì)干細胞成骨分化的影響。目的:探討外源性降鈣素基因相關肽對去勢大鼠骨髓間充質(zhì)干細胞增殖、成骨能力的影響。方法:切除雌性SD大鼠雙側(cè)卵巢建立骨質(zhì)疏松模型,并通過micro-CT驗證。從假手術和去勢大鼠股骨中提取骨髓間充質(zhì)干細胞,培養(yǎng)液中添加不同濃度降鈣素基因相關肽,MTT法檢測細胞增殖能力。對骨髓間充質(zhì)干細胞進行成骨誘導,在誘導液中添加不同濃度降鈣素基因相關肽,成骨誘導7 d,采用Real time RT-PCR和Western blot檢測成骨特異性蛋白Runx2,Osterix的表達,成骨誘導21 d進行茜素紅染色。結(jié)果與結(jié)論:(1)切除雌性大鼠雙側(cè)卵巢可成功建立骨質(zhì)疏松模型;(2)不同濃度的降鈣素基因相關肽可增強去勢大鼠骨髓間充質(zhì)干細胞的增殖及成骨分化能力,且具有劑量依賴性;(3)結(jié)果表明,降鈣素基因相關肽對去勢大鼠骨髓間充質(zhì)干細胞增殖及成骨分化能力有直接促進作用,從而有利于骨形成。
[Abstract]:Background: calcitonin gene-related peptide (CGRP) has been found to enhance the biological activity of osteoblasts. The effect of calcitonin gene-related peptide on osteogenic differentiation of bone marrow mesenchymal stem cells was studied. Aim: to investigate the effects of exogenous calcitonin gene related peptide (CGRP) on proliferation and osteogenesis of bone marrow mesenchymal stem cells (MSCs) in ovariectomized rats. Methods: Osteoporosis model was established by excision of bilateral ovaries of female SD rats and verified by micro-CT. Bone marrow mesenchymal stem cells (BMSCs) were extracted from sham-operated and ovariectomized rats femur. The proliferation of MSCs was detected by MTT assay with different concentrations of calcitonin gene-related peptide. Bone marrow mesenchymal stem cells (BMSCs) were induced by osteogenesis. Calcitonin gene-related peptide (CGRP) was added to the induction fluid for 7 days. Real time RT-PCR and Western blot were used to detect the expression of the osteoblast specific protein Runx2Osterix. Alizarin red staining was performed on 21 days after osteogenesis induction. Results and conclusion Osteoporosis model could be established successfully by removing female rat bilateral ovaries. Different concentrations of calcitonin gene-related peptide could enhance the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats. The results showed that calcitonin gene-related peptide could directly promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats.
【作者單位】： 軍事口腔醫(yī)學國家重點實驗室口腔疾病國家臨床醫(yī)學研究中心陜西省口腔疾病重點實驗室第四軍醫(yī)大學口腔醫(yī)院口腔外科;解放軍蘭州軍區(qū)總醫(yī)院頜面外科;
【基金】：國家自然科學基金(81271103) 陜西省社發(fā)攻關課題(2012K16-10-01)~~
【分類號】：R329.2

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