本文選題：朝鮮族和漢族 + Y-STR�。� 參考：《延邊大學(xué)》2012年碩士論文

【摘要】：目的：研究Y染色體6個(gè)STR基因座DYS19、DYS388、DYS390、DYS391、DYS392、DY8393的遺傳多態(tài)性在中國朝鮮族男性和漢族男性人群中的分布特征,并為法醫(yī)學(xué)個(gè)體識(shí)別、親權(quán)鑒定、人類遺傳學(xué)及其他相關(guān)學(xué)科提供參考數(shù)據(jù)。 方法：(1)收集200名無血緣關(guān)系的朝鮮族男性和200名漢族男性人群的抗凝血,用小量全血基因組DNA快速提取試劑盒提取DNA。(2)采用PCR擴(kuò)增結(jié)合5%聚丙烯酰胺凝膠電泳技術(shù)和銀染顯帶方法對200名朝鮮族男性和200名漢族男性的DYS19、DYS388、DYS390、DYS391、DYS392、DYS393基因座進(jìn)行檢測,并用Arlequin2000軟件進(jìn)行數(shù)據(jù)分析.(3)對6個(gè)基因座的等位基因分別進(jìn)行測序,得到等位基因分型標(biāo)準(zhǔn)物,并根據(jù)國際法醫(yī)遺傳學(xué)會(huì)DNA委員會(huì)(International Society of Forensic Genetics ISFG.DNA Commission)推薦命名. 結(jié)果：基因座DYS19、DYS388、DYS390、DYS391、DYS392、DYS393,在朝鮮族男性人群中依次檢測到6、4、5、5、6、5個(gè)等位基因,遺傳多態(tài)性(gene diversityGD)依次為0.6722、0.4848、0.6674、0.2641、0.6704、0.6405,將這6個(gè)Y-STR基因座累計(jì)分析,一共檢測到109種單倍型,累計(jì)GD值為0.9803；在漢族男性人群中依次檢測到6、5、7、5、7、5個(gè)等位基因,遺傳多態(tài)性(gene diversityGD)依次為0.7130、0.5219、0.6971、0.3509、0.7439、0.6564,將6個(gè)基因座累計(jì)分析,一共檢測到133種單倍型,累計(jì)GD值為0.9914。 結(jié)論：從本文數(shù)據(jù)顯示,中國朝鮮族男性和漢族男性人群6個(gè)Y-STR基因座,除DYS391遺傳多態(tài)性比較低外,其他基因座遺傳多態(tài)性分布較好,穩(wěn)定性強(qiáng),具有較高的應(yīng)用價(jià)值,可為中國朝鮮族男性和漢族男性人群的法醫(yī)學(xué)個(gè)人識(shí)別,親權(quán)鑒定,遺傳學(xué)及其他相關(guān)學(xué)科提供參考數(shù)據(jù)。
[Abstract]:Objective: to study the distribution of the genetic polymorphism of DYS19 / DYS388nDYS390DYS391and DYS392DY8393 in 6 STR loci of Y chromosome in Chinese male population of Korean and Han nationality, and to identify them as forensic individuals and to identify their paternity, and to investigate the relationship between DYS388and DYS390DYS391and DYS392DYS392DY8393. Human genetics and other related disciplines provide reference data. Methods the anticoagulant blood samples were collected from 200 unrelated Korean males and 200 Han males. The DYS19DYS388DYS391DYS391DYS392DYS392DYS391DYS391DYS392DYS393 locus of 200 Korean and 200 Han Chinese men were detected by PCR amplification, 5% polyacrylamide gel electrophoresis and silver staining. The alleles of 6 loci were sequenced with Arlequin2000 software, and the standard alleles were obtained and named according to the recommendations of the DNA Committee of the International Society of Forensic Genetics. Results: DYS19, DYS388, DYS390, DYS391DYS392and DYS393 were detected in the Korean male population, and 5 alleles were detected in turn, and the genetic polymorphism gene diversityGDwas 0.672222 0.48480.66774 (0.2641), 0.670441 (0.670441) and 0.6704405. the six Y-STR loci were analyzed and 109 haplotypes were detected. The accumulative GD value was 0.9803, and the genetic polymorphism gene diversityGDwas 0.7130 / 0.52190.6971 / 0.35090.439/ 0.65644.A total of 133 haplotypes were detected in the six loci of Han nationality, with a total of 133 haplotypes (GD = 0.9914). Conclusion: according to the data of this paper, there are 6 Y-STR loci in Chinese Korean and Han male populations, except for the low DYS391 genetic polymorphism, the other loci are more stable and have higher application value. It can provide reference data for forensic personal identification, paternity identification, genetics and other related subjects of Chinese Korean and Han male populations.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R394

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本文編號(hào)：1876379