本文選題：關(guān)節(jié)炎 + 膝關(guān)節(jié)　； 參考：《中國組織工程研究》2017年24期

【摘要】：背景:良好的動物模型是研究痛風(fēng)性關(guān)節(jié)炎重要的實(shí)驗(yàn)方法和手段。然而,目前尚無十分理想的痛風(fēng)性關(guān)節(jié)炎動物模型,這給尋找新的藥物、方法治療痛風(fēng)性關(guān)節(jié)炎帶來阻礙。目的:評價改良后的兔膝關(guān)節(jié)痛風(fēng)模型的有效性。方法:選取新西蘭大白兔隨機(jī)分成4組。空白對照組不做任何處理,右膝作為觀察側(cè);假手術(shù)組取大網(wǎng)膜,處理后不包裹單鈉尿酸鹽結(jié)晶,單純將大網(wǎng)膜植入兔右側(cè)膝關(guān)節(jié)髕上囊腔;經(jīng)典模型組將單鈉尿酸鹽結(jié)晶混懸液(0.1 g/L)注射入兔右側(cè)膝關(guān)節(jié)髕上囊腔;改良模型組取大網(wǎng)膜,處理后包裹單鈉尿酸鹽結(jié)晶(100 mg/kg),植入兔右側(cè)膝關(guān)節(jié)髕上囊腔。觀察造模后1,2,3 d及造模后1,2,3,4周關(guān)節(jié)腫脹程度、活動情況及關(guān)節(jié)炎癥反應(yīng)等來評估改良模型的優(yōu)缺點(diǎn)。結(jié)果與結(jié)論:(1)造模后4周內(nèi)各個時間點(diǎn)改良模型組的兔關(guān)節(jié)功能均顯著降低(P0.05);(2)經(jīng)典模型組和改良模型組造模后關(guān)節(jié)腫脹指數(shù)分別在造模后第1或2天達(dá)到高峰,其后均開始下降,而改良模型組較經(jīng)典模型組下降緩慢,造模后第4周時與空白對照組相比存在顯著性差異;(3)大體觀察:改良模型組和經(jīng)典模型組均呈現(xiàn)出明顯的關(guān)節(jié)炎癥改變:滑膜廣泛增生,伴充血水腫;關(guān)節(jié)腔內(nèi)有較多關(guān)節(jié)液形成;周圍組織充血水腫;軟骨未見明顯破壞;(4)經(jīng)典模型組和改良模型組造模后血清和關(guān)節(jié)液中白細(xì)胞介素6及白細(xì)胞介素1β水平分別在術(shù)后第1和第2天達(dá)到高峰,其后均開始下降,改良模型組下降較經(jīng)典模型組緩慢,至造模后4周時仍高于其他3組(P0.05);(5)結(jié)果說明,通過向兔膝關(guān)節(jié)腔內(nèi)植入用自體大網(wǎng)膜包裹的單鈉尿酸鹽結(jié)晶可以成功地制造兔膝關(guān)節(jié)痛風(fēng)性關(guān)節(jié)炎的模型,模型可維持至4周以上,較經(jīng)典模型維持時間顯著延長。
[Abstract]:Background: good animal models are important experimental methods and means to study gouty arthritis. However, there is no ideal animal model of gouty arthritis, which hinders the search for new drugs to treat gouty arthritis. Objective: to evaluate the effectiveness of modified rabbit knee joint gout model. Methods: new Zealand white rabbits were randomly divided into 4 groups. The blank control group did not do any treatment, the right knee was used as the observation side, the sham-operation group took the greater omentum, and after the treatment, the greater omentum was implanted into the right superior patellar sac of the right knee without encapsulating the single sodium uric acid crystal. In the classical model group, 0.1 g / L monosodium uric acid crystal suspension was injected into the right superior patellar capsule of the rabbit knee, and the modified model group was treated with the greater omentum and encapsulated with 100 mg / kg monosodium uric acid crystals and implanted into the right superior patellar capsule of the knee joint. To evaluate the advantages and disadvantages of the modified model, the degree of joint swelling, the activity and the reaction of arthritis were observed on the 3rd day after the establishment of the model and the 3th week after the establishment of the model. Results and conclusion the articular function of the modified model group was significantly decreased at each time point within 4 weeks after the establishment of the model. The joint swelling index of the classical model group and the modified model group reached the peak on the 1st and 2nd day after modeling, respectively. After that, both of them began to decrease, while the modified model group decreased more slowly than the classical model group. There was significant difference between the model group and the blank control group at the 4th week after modeling. The general observation showed that both the modified model group and the classical model group showed obvious changes of arthritis: extensive synovial hyperplasia with hyperemia and edema; There were more joint fluid formation in articular cavity, hyperemia and edema in surrounding tissue; The levels of interleukin 6 and interleukin 1 尾 in serum and joint fluid in the classical model group and the modified model group reached their peak on the first and second day after operation, and then began to decrease. The decline of the modified model group was slower than that of the classical model group, and was still higher than that of the other three groups at 4 weeks after the establishment of the model. The rabbit model of knee joint gouty arthritis can be successfully made by implantation of mononaturate crystals wrapped in autologous greater omentum into the rabbit knee joint cavity. The model can be maintained for more than 4 weeks, and the maintenance time of the model is significantly longer than that of the classical model.
【作者單位】： 四川大學(xué)華西醫(yī)院骨科運(yùn)動醫(yī)學(xué)中心;
【基金】：四川省科技廳支撐計(jì)劃(2013SZ0019)~~
【分類號】：R-332;R589.7

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