本文選題：傷口愈合 + 粒-巨噬細胞集落刺激因子(GMCSF)��； 參考：《重慶醫(yī)科大學(xué)》2011年碩士論文

【摘要】：目的:探討創(chuàng)面愈合過程中粒-巨噬細胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)的表達變化及其在創(chuàng)面愈合中的作用和機制。 方法:采用小鼠全層皮膚缺損傷模型。局部麻醉后起效后在背部中線近頸側(cè)制作1.0cm×1.0 cm大小全層皮膚缺損傷創(chuàng)面。50只雄性昆明小鼠隨機分為2組:對照組(25只)和GM-CSF實驗組(25只),每組分為5個時間點(每個時相點5只)。實驗組創(chuàng)面用rhGM-CSF(recombinant human granulocyte-macrophage colony stimulating factor)凝膠(10μg/cm2)涂抹,對照組創(chuàng)面用凝膠基質(zhì)涂抹。于傷后第3,5,7,10和14天,觀察創(chuàng)面愈合時間;計算創(chuàng)面愈合率;切取創(chuàng)面組織,檢測病理組織學(xué)變化;通過CD31免疫組化染色結(jié)果分析,計算創(chuàng)面微血管密度;應(yīng)用RT-PCR檢測創(chuàng)面GM-CSF、血小板源性生長因子(Platelet-derived growth factor,PDGF)、血管內(nèi)皮生長因子(Vascular endothelial growth factor,VEGF)和基質(zhì)細胞衍生因子-1(Stromal cell derived factor-1,SDF-1)基因表達變化。 結(jié)果:RT-PCR檢測結(jié)果顯示小鼠致創(chuàng)后創(chuàng)面GM-CSF基因表達傷后3d達峰值(P0.01),直到傷后10d均維持較高水平(P0.05),傷后14d其表達顯著下調(diào)接近正常;應(yīng)用rhGM-CSF凝膠后,創(chuàng)面愈合時間較對照組平均提前(2.4±0.27)d,其創(chuàng)面愈合率于傷后7～14d顯著升高( P0.05);組織學(xué)顯示創(chuàng)傷早期創(chuàng)面中性粒細胞數(shù)量較少,創(chuàng)沿上皮細胞增殖數(shù)量較多,創(chuàng)面肉芽組織增生明顯,并且細胞密度大以梭形細胞和卵圓形細胞為主,新生血管數(shù)量較多;創(chuàng)面微血管密度在傷后7~14d顯著增加(P0.05);VEGF和SDF-1基因分別于傷后7d和10d內(nèi)表達顯著上調(diào)(P 0.05),促愈生長因子PDGF基因于致傷后14d內(nèi)各時相點都明顯上調(diào)表達(5d,7d,10d,P0.05;14d,P0.01)。 結(jié)論:GM-CSF在創(chuàng)面愈合早期表達增高,GM-CSF可促進創(chuàng)面愈合,其作用機制與PGDF、VEGF、SDF-1基因表達上調(diào)有關(guān)。
[Abstract]:Objective: to investigate the expression and mechanism of granulocyte-macrophage colony stimulating factor GM-CSF in wound healing. Methods: the model of skin deficiency injury in mice was used. After local anesthesia, 50 male Kunming mice were randomly divided into two groups: control group (n = 25) and GM-CSF group (n = 25). Each group was divided into 5 time points. Each time point is 5. The wounds in the experimental group were smeared with rhGM-CSF(recombinant human granulocyte-macrophage colony stimulating factor (10 渭 g / cm ~ 2), while the wounds in the control group were smeared with gel matrix. The wound healing time was observed, the wound healing rate was calculated, the histopathological changes were detected by excision of wound tissue, and the microvessel density of the wound was calculated by CD31 immunohistochemical staining. The expression of GM-CSF, platelet-derived growth factor-1 (PDGF), vascular endothelial growth factor (VEGF) and stromal cell derived factor-1 (SDF-1) were detected by RT-PCR. Results the results of RT-PCR showed that the expression of GM-CSF gene reached a peak value 3 days after injury in mice wound, and remained at a high level until 10 days after injury, and the expression of P0.05 mRNA was significantly down-regulated at 14 days after injury, and the expression of rhGM-CSF gel was similar to that of normal. The wound healing time was 2.4 鹵0.27 days earlier than that in the control group, and the wound healing rate increased significantly at 7d after injury (P0.05). Histology showed that the number of neutrophils was less, the number of wound epithelial cells was more, and the granulation tissue proliferation was obvious in the early stage of trauma. The cell density was mainly fusiform cells and oval cells, and the number of neovascularization was more. The microvessel density of wound increased significantly within 7 and 10 days after injury, respectively, and the expression of PDGF gene increased significantly within 7 days and 10 days after injury, respectively, and the expression of PDGF gene was significantly up-regulated at all time points within 14 days after injury, including P0.0514d P0.0514d, P0.0514d, P0.0514d, P0.0514d, P0.0514d, P0.0514d, P0.0514d, P0.0514d, P0.0514d, P0.0514d, P0.0514d, respectively. Conclusion the increased expression of GM-CSF in the early stage of wound healing may promote wound healing, and the mechanism is related to the up-regulation of PGDFV EGF-1 SDF-1 gene expression.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R363

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